scholarly journals Homologous Recombination in Plant Cells after Agrobacterium-Mediated Transformation

1990 ◽  
Vol 2 (5) ◽  
pp. 415 ◽  
Author(s):  
Kathleen Y. Lee ◽  
Peter Lund ◽  
Keith Lowe ◽  
Pamela Dunsmuir
Keyword(s):  
Homologous Recombination ◽  
Plant Cells ◽  
Agrobacterium Mediated Transformation

scholarly journals Homologous recombination in plant cells after Agrobacterium-mediated transformation.

1990 ◽  
Vol 2 (5) ◽  
pp. 415-425 ◽  
Author(s):  
K Y Lee ◽  
P Lund ◽  
K Lowe ◽  
P Dunsmuir
Keyword(s):  
Homologous Recombination ◽  
Plant Cells ◽  
Agrobacterium Mediated Transformation

scholarly journals Extrachromosomal homologous recombination and gene targeting in plant cells after Agrobacterium mediated transformation.

1990 ◽  
Vol 9 (10) ◽  
pp. 3077-3084 ◽  
Author(s):  
R. Offringa ◽  
M. J. de Groot ◽  
H. J. Haagsman ◽  
M. P. Does ◽  
P. J. van den Elzen ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Gene Targeting ◽  
Plant Cells ◽  
Agrobacterium Mediated Transformation

Agrobacterium-Mediated Transformation Of Plant Cells

2018 ◽  
Author(s):  
Christine Kronfoth ◽  
◽  
Peter Grayson ◽  
Keyword(s):  
Plant Cells ◽  
Agrobacterium Mediated Transformation

scholarly journals Telomere Loops and Homologous Recombination-Dependent Telomeric Circles in a Kluyveromyces lactis Telomere Mutant Strain

2007 ◽  
Vol 28 (1) ◽  
pp. 20-29 ◽  
Author(s):  
Anthony J. Cesare ◽  
Cindy Groff-Vindman ◽  
Sarah A. Compton ◽  
Michael J. McEachern ◽  
Jack D. Griffith
Keyword(s):  
Electron Microscopy ◽  
Homologous Recombination ◽  
Mutant Strain ◽  
Kluyveromyces Lactis ◽  
Plant Cells ◽  
Physical Characteristics ◽  
Dna Molecules ◽  
Telomeric Dna ◽  
Strand Invasion

ABSTRACT The Kluyveromyces lactis ter1-16T strain contains mutant telomeres that are poorly bound by Rap1, resulting in a telomere-uncapping phenotype and significant elongation of the telomeric DNA. The elongated telomeres of ter1-16T allowed the isolation and examination of native yeast telomeric DNA by electron microscopy. In the telomeric DNA isolated from ter1-16T, looped molecules were observed with the physical characteristics of telomere loops (t-loops) previously described in mammalian and plant cells. ter1-16T cells were also found to contain free circular telomeric DNA molecules (t-circles) ranging up to the size of an entire telomere. When the ter1-16T uncapping phenotype was repressed by overexpression of RAP1 or recombination was inhibited by deletion of rad52, the isolated telomeric DNA contained significantly fewer t-loops and t-circles. These results suggest that disruption of Rap1 results in elevated recombination at telomeres, leading to increased strand invasion of the 3′ overhang within t-loop junctions and resolution of the t-loop junctions into free t-circles.

scholarly journals The role of double-strand break-induced allelic homologous recombination in somatic plant cells

2002 ◽  
Vol 32 (3) ◽  
pp. 277-284 ◽  
Author(s):  
Brigitte Gisler ◽  
Siegfried Salomon ◽  
Holger Puchta
Keyword(s):  
Homologous Recombination ◽  
Plant Cells ◽  
Strand Break ◽  
Double Strand Break

scholarly journals Overexpression of the HspL Promotes Agrobacterium tumefaciens Virulence in Arabidopsis Under Heat Shock Conditions

2015 ◽  
Vol 105 (2) ◽  
pp. 160-168 ◽  
Author(s):  
Hau-Hsuan Hwang ◽  
Yin-Tzu Liu ◽  
Si-Chi Huang ◽  
Chin-Yi Tung ◽  
Fan-Chen Huang ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Heat Shock ◽  
Transformation Efficiency ◽  
Plant Cells ◽  
Small Heat Shock Protein ◽  
Protein Accumulation ◽  
Transient Transformation ◽  
Root Explants ◽  
Agrobacterium Mediated Transformation ◽  
The Impact

Agrobacterium tumefaciens transfers a specific DNA fragment from the resident tumor-inducing (Ti) plasmid and effector virulence (Vir) proteins to plant cells during infection. A. tumefaciens VirB1-11 and VirD4 proteins assemble as the type IV secretion system (T4SS), which mediates transfer of the T-DNA and effector Vir protein into plant cells, thus resulting in crown gall disease in plants. Previous studies revealed that an α-crystallin-type, small heat-shock protein (HspL) is a more effective VirB8 chaperone than three other small heat-shock proteins (HspC, HspAT1, and HspAT2). Additionally, HspL contributes to efficient T4SS-mediated DNA transfer and tumorigenesis under room-temperature growth. In this study, we aimed to characterize the impact of HspL on Agrobacterium-mediated transformation efficiency under heat-shock treatment. During heat shock, transient transformation efficiency and VirB8 protein accumulation were lower in the hspL deletion mutant than in the wild type. Overexpression of HspL in A. tumefaciens enhanced the transient transformation efficiency in root explants of both susceptible and recalcitrant Arabidopsis ecotypes. In addition, the reduced transient transformation efficiency during heat stress was recovered by overexpression of HspL in A. tumefaciens. HspL may help maintain VirB8 homeostasis and elevate Agrobacterium-mediated transformation efficiency under both heat-shock and nonheat-shock growth.

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