Alterations of Endogenous Cytokinins in Transgenic Plants Using a Chimeric Isopentenyl Transferase Gene

1989 ◽  
Vol 1 (4) ◽  
pp. 403 ◽  
Author(s):  
June I. Medford ◽  
Roger Horgan ◽  
Zaki El-Sawi ◽  
Harry J. Klee
Keyword(s):  
Transgenic Plants ◽  
Isopentenyl Transferase ◽  
Transferase Gene ◽  
Endogenous Cytokinins

scholarly journals Selection of marker-free transgenic plants using the isopentenyl transferase gene

1997 ◽  
Vol 94 (6) ◽  
pp. 2117-2121 ◽  
Author(s):  
H. Ebinuma ◽  
K. Sugita ◽  
E. Matsunaga ◽  
M. Yamakado
Keyword(s):  
Transgenic Plants ◽  
Isopentenyl Transferase ◽  
Transferase Gene ◽  
Marker Free ◽  
Selection Of

Isopentenyl transferase gene (ipt) downstream transcriptionally fused with gene expression improves the growth of transgenic plants

2009 ◽  
Vol 19 (2) ◽  
pp. 197-209 ◽  
Author(s):  
Jian-Chun Guo ◽  
Rui-Jun Duan ◽  
Xin-Wen Hu ◽  
Kai-Mian Li ◽  
Shao-Ping Fu
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Isopentenyl Transferase ◽  
Transferase Gene

scholarly journals Evaluation of Resistance to Gummy Stem Blight (Didymella bryoniae) Disease in ipt (Isopentenyl Transferase) Transformed Watermelon Plants

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 820E-821
Author(s):  
Fahrettin Goktepe ◽  
Harrison Hughes*
Keyword(s):  
Transgenic Plants ◽  
Infected Leaf ◽  
Wild Type ◽  
Didymella Bryoniae ◽  
Isopentenyl Transferase ◽  
Disease Symptoms ◽  
Stem Blight ◽  
Transformed Plants ◽  
Gummy Stem Blight ◽  
Run Off

Watermelon plants are susceptible to Gummy stem blight disease that considerably reduces yields worldwide. In order to develop non-specific resistance, watermelon cv. Crimson Sweet was transformed with copper inducible isopentenyl transferase (ipt), the rate-limiting step in cytokinin biosynthesis, gene via Agrobacterium tumafaciences (LBA4404). Transformed (ipt) and nontransformed plants were grown at approximately 28-30 °C day, 20-22 °C night and 16 hours daylight under greenhouse conditions. Once the plants initiated new growth both transgenic plants and wild type plants were sprayed with one of three different concentrations (0, 10 & 50 μm) of CuSO4. Plants were sprayed twice to run-off in a twenty-four hour time period before inoculation with the pathogen. Cultures of the pathogen Didymela bryonia (W353) were grown for about 3 weeks and an inoculum containing 105 conidia per mL was sprayed with the prepared suspension until initial run-off in a humidified chamber. The disease symptoms were evaluated after one week with resistance demonstrated in all treated transgenic plants. All nonsprayed transgenic and wild type plants showed similar disease symptoms. Infected leaf samples were surface sterilized and re-cultured on V8 medium. The characteristics of the recovered pathogen confirmed that it was identical to the stock culture of W353. The same experiment has been conducted on seedlings from transgenic (T1 generations) and non-transformed plants. The non-transformed seedlings showed the first disease symptoms on their cotyledons and lower leaves. Disease resistance was observed in seedlings of the treated transformed plants as compared to nontransformed ones.

Seed-specific expression of the isopentenyl transferase gene (ipt) in transgenic tobacco

1998 ◽  
Vol 25 (1) ◽  
pp. 53 ◽  
Author(s):  
Qing-Hu Ma ◽  
Ren Zhang ◽  
Charles H. Hocart ◽  
David S. Letham ◽  
Thomas J. V. Higgins
Keyword(s):  
Seed Protein ◽  
Specific Gene ◽  
Biosynthetic Gene ◽  
Seed Protein Content ◽  
Isopentenyl Transferase ◽  
Ipt Gene ◽  
Gene Encoding ◽  
Specific Expression ◽  
Endogenous Cytokinins ◽  
Seed Specific Expression

The Agrobacterium tumefaciens gene encoding isopentenyl transferase (ipt), a cytokinin biosynthetic gene, was fused to a promoter from a seed-specific gene, vicilin, and introduced into tobacco cells. Intact fertile plants were generated. The expression of the vicilin-ipt gene was shown to be confined to seed and resulted in enhanced levels of cytokinins in the developing seeds and increased seed protein content. Using a simplified quantification method, a significant increase in the levels of endogenous cytokinins was recorded at 16–21 days after flowering. The growth of the transgenic plants and the development of the seeds appeared to be normal.

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