The Tomato Cf-5 Disease Resistance Gene and Six Homologs Show Pronounced Allelic Variation in Leucine-Rich Repeat Copy Number

1998 ◽  
Vol 10 (11) ◽  
pp. 1915
Author(s):  
Mark S. Dixon ◽  
Kostas Hatzixanthis ◽  
David A. Jones ◽  
Kate Harrison ◽  
Jonathan D. G. Jones
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Copy Number ◽  
Allelic Variation ◽  
Disease Resistance Gene ◽  
Leucine Rich Repeat ◽  
Repeat Copy Number ◽  
Repeat Copy

scholarly journals The Tomato Cf-5 Disease Resistance Gene and Six Homologs Show Pronounced Allelic Variation in Leucine-Rich Repeat Copy Number

1998 ◽  
Vol 10 (11) ◽  
pp. 1915-1925 ◽  
Author(s):  
Mark S. Dixon ◽  
Kostas Hatzixanthis ◽  
David A. Jones ◽  
Kate Harrison ◽  
Jonathan D. G. Jones
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Copy Number ◽  
Allelic Variation ◽  
Disease Resistance Gene ◽  
Leucine Rich Repeat ◽  
Repeat Copy Number ◽  
Repeat Copy

Map-based cloning of a gene sequence encoding a nucleotide-binding domain and a leucine-rich region at the Cre3 nematode resistance locus of wheat

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 659-665 ◽  
Author(s):  
Evans S. Lagudah ◽  
Odile Moullet ◽  
Rudi Appels
Keyword(s):  
Disease Resistance ◽  
Gene Family ◽  
Binding Site ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Gene Sequence ◽  
Nucleotide Binding ◽  
Disease Resistance Gene ◽  
Leucine Rich Repeat ◽  
Rich Region

The Cre3 gene confers a high level of resistance to the root endoparasitic nematode Heterodera avenae in wheat. A DNA marker cosegregating with H. avenae resistance was used as an entry point for map-based cloning of a disease resistance gene family at the Cre3 locus. Two related gene sequences have been analysed at the Cre3 locus. One, identified as a cDNA clone, encodes a polypeptide with a nucleotide binding site (NBS) and a leucine-rich region; this member of the disease resistance gene family is expressed in roots. A second Cre3 gene sequence, cloned as genomic DNA, appears to be a pseudogene, with a frame shift caused by a deletion event. These two genes, related to members of the cytoplasmic NBS – leucine rich repeat class of plant disease resistance genes were physically mapped to the distal 0.06 fragment of the long arm of wheat chromosome 2D and cosegregated with nematode resistance.Key words: cereal cyst nematode, disease resistance genes, nucleotide-binding site, leucine-rich repeat.

scholarly journals A pair of orthologs of a leucine-rich repeat receptor kinase-like disease resistance gene family regulates rice response to raised temperature

2011 ◽  
Vol 11 (1) ◽  
pp. 160 ◽  
Author(s):  
Haitao Zhang ◽  
Yinglong Cao ◽  
Jing Zhao ◽  
Xianghua Li ◽  
Jinghua Xiao ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Gene Family ◽  
Resistance Gene ◽  
Disease Resistance Gene ◽  
Receptor Kinase ◽  
Leucine Rich Repeat

Genetic mapping of plant disease resistance gene homologues using a minimal Brassica napus L. population

Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 735-743 ◽  
Author(s):  
A Joyeux ◽  
M G Fortin ◽  
R Mayerhofer ◽  
A G Good
Keyword(s):  
Disease Resistance ◽  
Brassica Napus ◽  
Genetic Mapping ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Mapping Population ◽  
Disease Resistance Gene ◽  
Leucine Rich Repeat ◽  
Repeat Domain ◽  
Polymerase Chain

Genetic mapping of plants traditionally involves the analysis of large segregating populations. However, not all individuals in a population contribute equal amounts of genetic information. It is thus possible to achieve rough mapping using a subset of the most informative individuals in the population. We have designed a minimal Brassica napus mapping population of 23 doubled-haploid plants and have tested this method using this population in the mapping of disease resistance gene homologues in B. napus. Several groups have identified such homologues in soybean and potato by amplifying sequences corresponding to conserved nucleotide-binding sites from known resistance genes. However, the sequence conservation in the leucine-rich repeat domain that is present in most of the disease resistance genes isolated has not been exploited via the polymerase chain reaction (PCR). We present the genetic mapping of Brassica napus DNA sequences amplified with primers corresponding to both the nucleotide-binding site and the leucine rich-repeat domain of the Arabidopsis thaliana RPS2 gene. We also describe a method for the quick mapping of resistance gene homologues using the polymerase chain reaction.Key words: Brassica napus, disease resistance genes, minimal mapping population, RFLP markers.

scholarly journals Isolation, Sequence Analysis, and Linkage Mapping of Nucleotide Binding Site–Leucine-rich Repeat Disease Resistance Gene Analogs in Watermelon

2009 ◽  
Vol 134 (6) ◽  
pp. 649-657 ◽  
Author(s):  
Karen R. Harris ◽  
W. Patrick Wechter ◽  
Amnon Levi
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Linkage Mapping ◽  
Citrullus Lanatus ◽  
Nucleotide Binding ◽  
Disease Resistance Gene ◽  
Resistance Gene Analogs ◽  
Leucine Rich Repeat ◽  
Degenerate Primers ◽  
Sequence Tagged Sites

Sixty-six watermelon (Citrullus lanatus var. lanatus) disease resistance gene analogs were cloned from ‘Calhoun Gray’, PI 296341, and PI 595203 using degenerate primers to select for the nucleotide binding sites (NBS) from the NBS–leucine-rich repeat (LRR) resistance gene family. After contig assembly, watermelon resistance gene analogs (WRGA) were identified and amino acid sequence alignment revealed that these groups contained motifs characteristic of NBS-LRR resistance genes. Using cluster analysis, eight groups of WRGA were identified and further characterized as having homology to Drosophila Toll and mammalian interleukin-1 receptor (TIR) and non-TIR domains. Three of these WRGA as well as three disease-related watermelon expressed sequence tag homologs were placed on a test-cross map. Linkage mapping placed the WRGA on linkage group XIII, an area on the watermelon map where resistance gene analogs cluster. In addition, these WRGA sequence-tagged sites (STS) were amplified from various genera of the Cucurbitaceae indicating that conservation of resistance gene analogs exists among cucurbits. These WRGA-STS markers may be useful in marker-assisted selection for the improvement for disease resistance in watermelon.

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