614-P: Optimal Control of Glucose and Lipid Profiles Is Critical to the Effective “Neo-Islet” Therapy of Insulin Dependent Pet Dogs

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 614-P
Author(s):  
ANNA GOOCH ◽  
SABIHA S. CHOWDHURY ◽  
CHRISTOF WESTENFELDER
Keyword(s):  
Optimal Control ◽  
Lipid Profiles ◽  
Pet Dogs ◽  
Insulin Dependent

scholarly journals First report on the effective intraperitoneal therapy of insulin-dependent Diabetes mellitus in pet dogs using “Neo-Islets,” aggregates of adipose stem and pancreatic islet cells

2019 ◽  
Author(s):  
Anna Gooch ◽  
Ping Zhang ◽  
Zhuma Hu ◽  
Natasha Loy Son ◽  
Nicole Avila ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Glycemic Control ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Immunosuppressive Agents ◽  
Intraperitoneal Administration ◽  
Pancreatic Islet Cells ◽  
Pet Dogs ◽  
Insulin Dependent

AbstractWe previously reported that allogeneic, intraperitoneally administered “Neo-Islets,” composed of cultured pancreatic islet cells co-aggregated with high numbers of immunoprotective and cytoprotective Adipose-derived Stem Cells, reestablished, through omental engraftment, redifferentiation and splenic and omental up-regulation of Regulatory T-cells, normoglycemia in autoimmune Type-1 Diabetic Non-Obese Diabetic (NOD) mice without the use of immunosuppressive agents or encapsulation devices. Based on these observations, we are currently testing this Neo-Islet technology in an FDA guided Pilot Study (INAD 012-776) in insulin-dependent, spontaneously diabetic pet dogs by the intraperitoneal administration of 2×10e5 Neo-Islets/kilogram body weight to metabolically controlled (blood glucose, triglycerides, thyroid and adrenal functions) animals under sedation and local anesthesia and ultrasound guidance. We report here initial observations on the first 4 Neo-Islet-treated, insulin dependent pet dogs that are now in the intermediate-term follow-up phase of the study (> 6 months post treatment). Current results indicate that in dogs, Neo-Islets appear to engraft, redifferentiate and physiologically produce insulin, and are neither rejected by auto- or allo-immune attacks, as evidenced by (a) an absent IgG response to the allogeneic cells contained in the administered Neo-Islets, and (b) progressively improved glycemic control that achieves up to a 50% reduction in daily insulin needs paralleled by a significant fall in serum glucose levels. This is accomplished without the use of anti-rejection drugs or encapsulation devices. No adverse or serious adverse events related to the Neo-Islet administration have been observed to date. We conclude that this minimally invasive therapy has significant translational relevance to veterinary and clinical Type 1 Diabetes Mellitus by achieving complete and at this point partial glycemic control in two species, i.e., diabetic mice and dogs, respectively.

scholarly journals Serum electrolytes and lipid profiles in non-insulin dependent diabetes mellitus patients

2014 ◽  
Vol 6 (3) ◽  
pp. 38-41
Author(s):  
Namama Talabani
Keyword(s):  
Diabetes Mellitus ◽  
Insulin Dependent Diabetes Mellitus ◽  
Lipid Profiles ◽  
Insulin Dependent Diabetes ◽  
Dependent Diabetes Mellitus ◽  
Group I ◽  
Insulin Dependent ◽  
The Mean ◽  
Significant Change ◽  
High Level

Background: Diabetes mellitus and hyperlipidemia are the most common metabolic disorder affecting the people all over the world. Hyperglycemia is considered a primary cause of diabetic vascular complications and is associated with impaired electrolytes in some of the metabolic dysfunctions is not clear. Aim: The purpose of this study was conducted to investigate the relationship among diabetes mellitus, lipid profiles and electrolytes (Na+, K+ and Cl-). Methods: In the sera of 85 non insulin-dependent diabetes mellitus NIDDM, 45 with hyperlipidemia, 40 without hyperlipidemia, 50 samples of hyperlipidemia without NIDDM, and 50 non diabetic healthy control subjects. The mean age of the diabetic patients was similar to that of control. The mean duration of the disease was (10.2±5.9) years (2-23) years. From the results, it was discovered that there was a significant decrease in Na+ and Cl- in patients with NIDDM without high level of lipid profile (group I), but our results show that the concentration of K+ not changed significantly. The plasma levels of Na+ and Cl- ions were show significant change in patient with hyperlipidemia without NIDDM (group II), while plasma K+ not changed significantly in this group as compared with control. The mean value of Na+ and Cl- show high significant change in NIDDM patients with high level of lipids profile (group III),were plasma K+ not changed significantly as compared with control group. Conclusion: These finding may explain the role of impaired electrolytes status in NIDDM and hyperlipidemia subjects. DOI: http://dx.doi.org/10.3126/ajms.v6i3.11088Asian Journal of Medical Sciences Vol.6(3) 2015 38-41 

The Effect of an α-Glucosidase Inhibitor and Insulin on Glucose Metabolism and Lipid Profiles in Non-Insulin-Dependent Diabetes Mellitus

1996 ◽  
Vol 24 (5) ◽  
pp. 438-447 ◽  
Author(s):  
S Okada ◽  
K Ishii ◽  
H Hamada ◽  
S Tanokuchi ◽  
K Ichiki ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Insulin Dose ◽  
Insulin Dependent Diabetes Mellitus ◽  
Lipid Profiles ◽  
Glycosylated Haemoglobin ◽  
Postprandial Blood Glucose ◽  
Dependent Diabetes Mellitus ◽  
Glucosidase Inhibitor ◽  
Insulin Dependent

Studies were carried out to assess various ways of improving glycaemic control and lipid profiles of patients with non-insulin-dependent diabetes mellitus (NIDDM) in whom glucose metabolism was poor. Part or all of the dose of the sulphonylurea that had been used to treat patients in Group 1 ( n = 8) was replaced by an α-glucosidase inhibitor. Symptoms related to hypoglycaemia disappeared and the postprandial blood glucose level was significantly increased ( P < 0.043) but serum lipid levels were not significantly altered and the mean glycosylated haemoglobin level was unchanged. In Group 2 ( n = 10) patients, a large part of the insulin dose was replaced by an α-glucosidase inhibitor. Hypoglycaemia-related symptoms disappeared but there were no significant changes in lipid profiles, postprandial blood glucose or glycosylated haemoglobin levels. The third group of patients ( n = 9) had been treated with insulin alone and were given additional α-glucosidase inhibitor without changing their insulin dose. This did not significantly change their lipid profiles, postprandial blood glucose or glycosylated haemoglobin levels. In Group 4 ( n = 9) the addition of an α-glucosidase inhibitor to the initial sulphonylurea did not produce any significant changes in mean postprandial blood glucose or glycosylated haemoglobin levels. The results for individual patients indicated that the glycosylated haemoglobin levels had improved after the change of treatment only in those patients whose connective peptide immunoreactivity was > 6.0 ng/ml.

Low-voltage field-emission SEM of polymeric membranes for glucose biosensors

1990 ◽  
Vol 48 (3) ◽  
pp. 860-861
Author(s):  
Lorna K. Mayo ◽  
Kenneth C. Moore ◽  
Mark A. Arnold
Keyword(s):  
Glucose Sensor ◽  
Low Voltage ◽  
Glucose Sensors ◽  
Polymeric Membranes ◽  
Artificial Endocrine Pancreas ◽  
Self Monitoring ◽  
Conducting Materials ◽  
Insulin Dependent ◽  
Living Tissues ◽  
Voltage Scanning

An implantable artificial endocrine pancreas consisting of a glucose sensor and a closed-loop insulin delivery system could potentially replace the need for glucose self-monitoring and regulation among insulin dependent diabetics. Achieving such a break through largely depends on the development of an appropriate, biocompatible membrane for the sensor. Biocompatibility is crucial since changes in the glucose sensors membrane resulting from attack by orinter action with living tissues can interfere with sensor reliability and accuracy. If such interactions can be understood, however, compensations can be made for their effects. Current polymer technology offers several possible membranes that meet the unique chemical dynamics required of a glucose sensor. Two of the most promising polymer membranes are polytetrafluoroethylene (PTFE) and silicone (Si). Low-voltage scanning electron microscopy, which is an excellent technique for characterizing a variety of polymeric and non-conducting materials, 27 was applied to the examination of experimental sensor membranes.

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