A Case of Anaphylaxis in Which a Basophil Activation Test Was Used to Identify the Suspected Agent

This is a case report of anaphylaxis in which the basophil activation test (BAT) was used to identify the etiological agent. Although skin tests are considered the most effective methods for identifying anaphylactic triggers, the test itself presents a risk of inducing anaphylaxis. The BAT is advantageous because of its inherent lack of risk, high sensitivity and specificity to identify the suspected anaphylactic agents, and diagnostic accuracy comparable to conventional skin testing. Therefore, in the future, the BAT is likely to become the preferred test for the detection of allergens over conventional skin tests.

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Immediate allergic reaction to thiocolchicoside confirmed by skin testing and basophil activation test: A case report and literature review

Asian Pacific Journal of Allergy and Immunology ◽

10.12932/ap-250320-0793 ◽

2021 ◽

Keyword(s):

Case Report ◽

Literature Review ◽

Allergic Reaction ◽

Skin Testing ◽

Basophil Activation Test ◽

Basophil Activation ◽

Activation Test

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Application of basophil activation and degranulation markers for diagnosis of pollen allergy

Proceedings of the National Academy of Sciences of Belarus Medical series ◽

10.29235/1814-6023-2018-15-4-405-413 ◽

2019 ◽

Vol 15 (4) ◽

pp. 405-413

Author(s):

I. U. Ramanava ◽

A. Y. Hancharou ◽

N. I. Dudarava

Keyword(s):

Sensitivity And Specificity ◽

Pollen Allergy ◽

High Sensitivity ◽

Basophil Activation Test ◽

Point Of View ◽

Basophil Activation ◽

Pollen Allergens ◽

Activation Test ◽

Activation Marker ◽

Marker Cd107a

There are several basophil activation and degranulation markers for basophil activation test discovered in recent years. However, only CD63 and CD203c are actively used. The activation and degranulation markers (CD11b, CD13, CD63, CD69, CD107a, CD164, CD203c and CD300a) were characterized from the point of view of the possibility to use for BAT and their clinical efficiency. It has been shown that the degranulation marker CD107a and the activation marker CD11b have high sensitivity and specificity and can be used to diagnose hypersensitivity to pollen allergens.

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Deallergisation trials of pure celery juice and apple-celery juice mixture by oxidation

Czech Journal of Food Sciences ◽

10.17221/273/2010-cjfs ◽

2011 ◽

Vol 29 (No. 2) ◽

pp. 190-200 ◽

Cited By ~ 4

Author(s):

P. Novotná ◽

I. Šetinová ◽

M. Heroldová ◽

M. Kmínková ◽

J. Průchová ◽

...

Keyword(s):

Apple Juice ◽

No Reduction ◽

Skin Prick Testing ◽

Skin Testing ◽

Basophil Activation Test ◽

Basophil Activation ◽

Enzymatic Browning ◽

Colour Measurement ◽

Activation Test ◽

Natural Polyphenol

This work aimed to determine if it was possible to eliminate or reduce the content of the Api g1 allergen in celery juice by oxidation, utilising its natural polyphenol oxidase (PPO) content. We attempted to determine a possible relationship between the enzymatic browning of celery juice and the reactivity of the Api g1 allergen. Pressed celery juice was stirred, and samples for the colour measurement and allergenicity, determined using the Western Blot (WB) method, were collected at pre-defined times. Oxidation failed to eliminate the allergenicity of pure celery juice. Further trials were focused on celery allergen elimination in apple-celery juices mixtures in ratios of 3:1, 5:1, and 7:1. We selected the 5:1 ratio as the most acceptable from the sensory perspective, and monitored its allergenicity using the WB method, basophil activation test, and skin prick testing. The WB test showed that oxidation, caused by stirring for 120 min, reduced the allergenicity of the mixture. However, the basophil activation test showed no reduction in the allergic response to the oxidised juice mixture. Skin testing showed that the oxidised juice mixture stirred for 120 min exhibited a significantly lower reaction than the juice mixture stirred for 60 min or celery and apple juice stabilised with ascorbic acid. Due to the contradictory results in different tests, the method cannot be declared successful or safe, even for mixtures of apple-celery juices.

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