Sensitivity and specificity of passive immune‐basophil activation test to detect allergic transfusion reactions

Transfusion ◽  
2019 ◽  
Vol 59 (11) ◽  
pp. 3308-3313 ◽  
Author(s):  
Kazuta Yasui ◽  
Yoshihiro Takihara ◽  
Nobuki Matsuyama ◽  
Hidefumi Kato ◽  
Kazuhiko Oka ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test ◽  
Transfusion Reactions

Developing an in Vitro Functional Model of Allergic Transfusion Reactions

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 3426-3426
Author(s):  
William Savage ◽  
Aaron A. R. Tobian ◽  
Jessica Savage ◽  
Chris Thoburn ◽  
Paul M. Ness ◽  
...  
Keyword(s):  
Basophil Activation Test ◽  
Positive Control ◽  
Basophil Activation ◽  
Clinical Severity ◽  
Blue Staining ◽  
Activation Test ◽  
And Control ◽  
Transfusion Reactions ◽  
Positive Controls

Abstract Abstract 3426 Background: Allergic transfusion reactions (ATRs) occur in ∼2–3% of platelet transfusions, but their etiology remains largely unclear. Both donor and recipient factors have been shown to contribute to ATR risk. An in vitro model of ATRs would enable mechanistic studies. Objective: To develop a basophil activation test as a model of ATRs to apheresis platelets (AP) by measuring histamine release (HR) and leukotriene C4 (LTC4) production after exposing basophils to AP supernatants. Methods: Basophil-enriched suspensions (∼5–15% basophils) from healthy donors were prepared using double-Percoll density centrifugation. After washing and counting basophil numbers using Alcian blue staining, cells were resuspended in buffer containing 2 ng/mL IL-3 and 5mM Ca++/Mg++ such that 20–30×104 basophils were added in 0.025mL aliquots. IL-3 is a potent basophil priming agent and was used to model the increased recipient susceptibility to allergic reactions that has been observed clinically. Reaction tubes (1.5 mL) were set-up containing 0.025mL of 2× (twice the final conc.) of ATR/control supernatant (final titer 1:2–1:8), negative (buffer alone), and positive control reagents (100 ng/mL anti-IgE antibody, 50 ng/mL C5a, 1 μM FMLP). Cells and reaction tubes were pre-warmed separately to 37°C for 15' before aliquoting 0.025mL cell suspension to reaction tubes for a final volume of 0.050mL. After 30′ incubation at 37°C, 0.950mL of cold buffer (without IL-3 or Ca++/Mg++) was added to each reaction tube. Tubes were centrifuged 10" at ∼300g (4°C) before removing 0.900mL for histamine detection by automated fluorimetry and LTC4 detection by ELISA. AP supernatants that were (n=10) or were not (n=10) associated with ATRs were tested. HR values were determined as a percentage of the total histamine content by lysis with perchloric acid (1.6%). Results used in analysis are net HR or LTC4 production (AP sample minus IL-3 primed negative control) normalized to HR or LTC4 production observed with positive control. Results: IL-3 priming of basophils was necessary in order to evoke a response after exposure to AP supernatant. HR in response to AP incubation with unprimed basophils was <3% of positive controls. Maximal HR and LTC4 production in response to AP supernatants occurred at a final titer of 1:4 using IL-3 primed basophils. HR and LTC4 production within individual reactions were correlated (Spearman rho=0.83, P<0.0001). Mean net HR among all AP supernatant samples was 55±28%, 60±49%, and 53±29% of c5a, anti-IgE, and FMLP positive controls, respectively. LTC4 production was 19±26%, 14±22%, and 15±20% of c5a, anti-IgE, and FMLP positive controls. No statistically significant differences were observed in HR or LTC4 production when ATR and control groups were compared (P>0.1). ATRs were stratified into clinical categories of mild (pruritus/urticaria, n=3), moderate (angioedema/dyspnea, n=5), and severe (any symptom with hypotension, n=2). There were no differences or trends in HR and LTC4 production among the categories (P>0.2). There was no correlation between pre/post transfusion tryptase elevations in recipient plasma (n=4) and HR or LTC4 production. HR and LTC4 production was similar among blood types of AP products (A: n=12, O: n=5, AB: n=3), (P>0.7). We previously reported that concentrations of C5a are higher in AP products associated with ATRs than controls. C5a content in AP supernatant modestly correlated with basophil HR release (rho: 0.47, P=0.04), but less with LTC4 production (rho: 0.27, P=0.3). Summary: An in vitro basophil activation test that measures HR and LTC4 production was developed to study ATRs. We find that 1) IL-3 priming of basophils was necessary to elicit responses to AP supernatants; 2) LTC4 and HR are correlated within each reaction; 3) there are no differences in HR and LTC4 production between ATR and control AP supernatants were observed, regardless of clinical ATR severity; and 4) HR is associated with C5a content in the AP supernatant. Conclusions: The observations of 1) an overall requirement for basophil priming and 2) the lack of correlation between the clinical severity of ATRs and basophil HR and LTC4 release implicate recipient atopic priming as a risk factor for ATRs. However, the association of AP C5a content with HR suggests a donor/product role in ATRs, as well. Studies focusing on the nature of recipient susceptibility may further elucidate mechanisms of ATRs. Disclosures: No relevant conflicts of interest to declare.

Application of the basophil activation test in the analysis of allergic transfusion reactions

2009 ◽  
Vol 19 (5) ◽  
pp. 274-277 ◽  
Author(s):  
N. Matsuyama ◽  
F. Hirayama ◽  
S. Wakamoto ◽  
K. Yasui ◽  
R. A. Furuta ◽  
...  
Keyword(s):  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test ◽  
Transfusion Reactions

Clinical utility of the basophil activation test for analysis of allergic transfusion reactions: a pilot study

Vox Sanguinis ◽  
2017 ◽  
Vol 112 (2) ◽  
pp. 114-121 ◽  
Author(s):  
I. Okamura ◽  
N. Matsuyama ◽  
K. Yasui ◽  
F. Hirayama ◽  
T. Ikeda
Keyword(s):  
Pilot Study ◽  
Clinical Utility ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test ◽  
Transfusion Reactions

Clinical utility of a passive immune basophil activation test for the analysis of allergic transfusion reactions

Transfusion ◽  
2017 ◽  
Vol 57 (9) ◽  
pp. 2084-2095 ◽  
Author(s):  
Kazuta Yasui ◽  
Nobuki Matsuyama ◽  
Ikue Okamura-Shiki ◽  
Takashi Ikeda ◽  
Kazuyoshi Ishii ◽  
...  
Keyword(s):  
Clinical Utility ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test ◽  
Transfusion Reactions

scholarly journals Application of basophil activation and degranulation markers for diagnosis of pollen allergy

2019 ◽  
Vol 15 (4) ◽  
pp. 405-413
Author(s):  
I. U. Ramanava ◽  
A. Y. Hancharou ◽  
N. I. Dudarava
Keyword(s):  
Sensitivity And Specificity ◽  
Pollen Allergy ◽  
High Sensitivity ◽  
Basophil Activation Test ◽  
Point Of View ◽  
Basophil Activation ◽  
Pollen Allergens ◽  
Activation Test ◽  
Activation Marker ◽  
Marker Cd107a

There are several basophil activation and degranulation markers for basophil activation test discovered in recent years. However, only CD63 and CD203c are actively used. The activation and degranulation markers (CD11b, CD13, CD63, CD69, CD107a, CD164, CD203c and CD300a) were characterized from the point of view of the possibility to use for BAT and their clinical efficiency. It has been shown that the degranulation marker CD107a and the activation marker CD11b have high sensitivity and specificity and can be used to diagnose hypersensitivity to pollen allergens.

scholarly journals A Case of Anaphylaxis in Which a Basophil Activation Test Was Used to Identify the Suspected Agent

2020 ◽  
Vol 67 (3) ◽  
pp. 172-173
Author(s):  
Takashi Goto ◽  
Shintaro Hayashi ◽  
Hiroko Tsugane ◽  
Mitsuo Iinuma ◽  
Satoru Sakurai
Keyword(s):  
Case Report ◽  
Diagnostic Accuracy ◽  
Sensitivity And Specificity ◽  
Skin Testing ◽  
High Sensitivity ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Etiological Agent ◽  
Skin Tests ◽  
Activation Test

This is a case report of anaphylaxis in which the basophil activation test (BAT) was used to identify the etiological agent. Although skin tests are considered the most effective methods for identifying anaphylactic triggers, the test itself presents a risk of inducing anaphylaxis. The BAT is advantageous because of its inherent lack of risk, high sensitivity and specificity to identify the suspected anaphylactic agents, and diagnostic accuracy comparable to conventional skin testing. Therefore, in the future, the BAT is likely to become the preferred test for the detection of allergens over conventional skin tests.

scholarly journals Basophil activation: theoretical aspects and use in the diagnosis of allergic diseases

2021 ◽  
Vol 23 (3) ◽  
pp. 469-482
Author(s):  
N. V. Bychkova
Keyword(s):  
Sensitivity And Specificity ◽  
Allergic Inflammation ◽  
Allergic Diseases ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
Basophil Activation ◽  
Present Stage ◽  
Laboratory Methods ◽  
Activation Test

Diagnostics of allergic diseases is a difficult issue, which requires distinct solutions, since this disorder is very common among the population. The overview focuses on complex diagnostics, including various methods that are most in demand at the present stage. The allergy diagnostics primarily include taking anamnesis, physical examination, instrumental and functional tests. Less often, the provocative tests are used, due to risk of severe adverse reactions. At the present stage, the role of laboratory diagnostics of allergies is growing, since, firstly, there is an increase in difficult-to-diagnose cases that require involvement of the entire medical armamentarium, and, secondly, the sensitivity and specificity of laboratory tests are improving. Among laboratory methods, the most significant are the assessment of the level of specific IgE, and the relatively new basophile activation test. The latter test is the main focus of the present review. It is functional and combines the advantages of provocative tests, during which conditions are created for the interaction of a potential allergen and effector cells of allergic inflammation, keeping safety for the patient. The data on the life cycle of basophils, their expression of membrane receptors, the content of granules, and ability to produce additional inflammatory mediators by the cells are presented. Participation of these cells in pathogenesis of allergic inflammation is being considered. Various mechanisms of basophil activation are discussed, both IgE-mediated and IgE-independent, which are similar in vivo and in vitro. Theoretical aspects of using the in vitro basophil activation test to estimate the hypersensitivity to a wide range of allergens are discussed. High sensitivity and specificity of the test for diagnosing allergies to food, household, pollen, insect and drug allergens are presented. Specific features of the basophil activation test related to the preanalytical, analytical and postanalytical stages of the study are highlighted. The factors influencing evaluation of this method are known. For example, difficulties in interpreting the test may arise while taking glucocorticosteroid hormones, in acute period of inflammation, with severe edema. The possibility of using this test to assess effectiveness of allergen-specific and anti-IgE therapy is being considered. A comparison of the basophil activation test, measurement of specific IgE and skin tests by various parameters related to performance and interpretation of results is carried out. Comprehensive diagnostics of allergic diseases, including usage of pathogenetically determined laboratory methods, will contribute to adequate treatment and, as a result, improve the health of the population.

Possible Utility of the Basophil Activation Test for the Analysis of Mechanisms Involved in Allergic Transfusion Reactions

2018 ◽  
Vol 32 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Fumiya Hirayama ◽  
Kazuta Yasui ◽  
Nobuki Matsuyama ◽  
Ikue Okamura-Shiki
Keyword(s):  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test ◽  
Transfusion Reactions

Usefulness of basophil activation test for the diagnosis of peanut allergy in Japanese children

2014 ◽  
Vol 28 (2) ◽  
pp. 216-225
Author(s):  
Shigeyuki Narabayashi ◽  
Takaaki Meguro ◽  
Yasunori Ito ◽  
Fumika Tokunaga ◽  
Shiro Seto ◽  
...  
Keyword(s):  
Peanut Allergy ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Japanese Children ◽  
Activation Test
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