Basophil activation test and quantification of specific IgE versus skin tests in anaesthesia-related allergy

2010 ◽  
Vol 27 ◽  
pp. 238-239
Author(s):  
N. Hagau ◽  
S. Tranca ◽  
R. Hagau ◽  
L. Bene ◽  
C. Indrei
Keyword(s):  
Basophil Activation Test ◽  
Specific Ige ◽  
Basophil Activation ◽  
Skin Tests ◽  
Activation Test

scholarly journals BASOPHILE ACTIVATION TEST FOR THE DIAGNOSTICS OF FUNGAL SENSITIZATION IN THE PATIENTS WITH CYSTIC FIBROSIS

2019 ◽  
Vol 21 (5) ◽  
pp. 919-928
Author(s):  
Ya. I. Kozlova ◽  
E. V. Frolova ◽  
A. E. Uchevatkina ◽  
L. V. Filippova ◽  
O. V. Aak ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Stimulation Index ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
Basophil Activation ◽  
Total Ige ◽  
Activation Test ◽  
Fungal Allergens ◽  
Fungal Sensitization

Aspergillus fumigatus colonization in the patients with cystic fibrosis (CF) may cause sensitization against A. fumigatus and/or allergic bronchopulmonary aspergillosis (ABPA), which significantly worsens the course of underlying disease. At the present time, new diagnostic tests are searched for detection of fungal sensitization in these patients. The aim of this work was to evaluate an opportunity of application of basophile activation test with A. fumigatus allergen in vitro using flow cytometry, aiming for identification of fungal sensitization in the CF patients. The study included 190 patients with CF aged 1 to 37 years. All the patients underwent common allergy screening (skin tests with fungal allergens, determination of serum levels of total IgE and specific IgE for the fungal allergens), and mycological examination (microscopy and culture of respiratory substrates). Computed tomography of the chest was performed upon clinical indications. The basophil activation test with the A. fumigatus allergen was performed in 10 CF patients with ABPA, and 10 CF patients without ABPA, in addition to the standard allergological examination. Frequency of sensitization to A. fumigatus in the patients with cystic fibrosis was 27%, the incidence of allergic bronchopulmonary aspergillosis was 5.7%. The number of eosinophils, total IgE and specific IgE levels in CF patients with ABPA were significantly higher than in CF patients without ABPA. In blood of the ABPA patients we have identified 68.5 (52.5-81.5%) of basophilic leukocytes activated by A. fumigatus allergen, with a stimulation index of 17.07 (10.30-27.70). In appropriate comparison group, the stimulation index did not exceed 1.5 (p = 0.000). Direct positive correlation between the levels of specific IgE to A. fumigatus and the number of basophils activated by A. fumigatus allergens was revealed (r = 0.77; р < 0.05). FVC values and the body mass index in CF patients with ABPA were significantly lower when compared with the patients without fungal sensitization. Introduction of the basophil activation test, along with standard techniques, may enable a more differentiated assessment of ABPA development in CF patients. Timely detection of associations between A. fumigatus sensitization and clinical status of CF patients will facilitate early and effective administration of specific therapy.

scholarly journals Evaluation of Latex Subclinical Sensitization by Way of the Basophil Activation Test and Specific IgE to Latex Recombinant Allergens

2013 ◽  
Vol 62 (3) ◽  
pp. 385-387 ◽  
Author(s):  
Ana Rodríguez Trabado ◽  
Luis Miguel Fernández Pereira ◽  
Silvia Romero-Chala ◽  
José Antonio García-Trujillo ◽  
Carmen Cámara Hijón
Keyword(s):  
Basophil Activation Test ◽  
Specific Ige ◽  
Basophil Activation ◽  
Recombinant Allergens ◽  
Activation Test

S.45. Basophil Activation Test Compared to Skin Prick Test and Fluorescent Enzymoimmunoassay for Aeroallergen-specific IgE

2009 ◽  
Vol 131 ◽  
pp. S145
Author(s):  
Faisal Khan ◽  
Aito Ueno-Yamanouchi ◽  
Bazir Serushago ◽  
Tom Bowen ◽  
Andrew Lyon ◽  
...  
Keyword(s):  
Skin Prick Test ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
Basophil Activation ◽  
Activation Test ◽  
Prick Test

scholarly journals Clinical and immunological characteristics of severe bronchial asthma with Aspergillus sensitization

2021 ◽  
Vol 5 (1) ◽  
pp. 10-16
Author(s):  
Ya.I. Kozlova ◽  
◽  
E.V. Frolova ◽  
A.E. Uchevatkina ◽  
L.V. Filippova ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Bronchial Asthma ◽  
Stimulation Index ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
Forced Expiratory Volume ◽  
Basophil Activation ◽  
Activation Test ◽  
To Receive

Aim: to assess the clinical and immunological characteristics of patients with severe bronchial asthma (BA) with Aspergillus sensitization and to study the possibilities of applying basophil activation test (BAT) using flow cytometry to detect mycogenic sensitization. Patients and Methods: 93 patients with severe BA were examined. Therapy had 4 or 5 steps according to GINA 2019 recommendations. TARC (thymus and activation-regulated chemokine) content, the number of eosinophils, the levels of total IgE and specific IgE to 10 allergens were determined in blood serum by enzyme immunoassay. BAT with Aspergillus fumigatus allergen was performed in vitro using flow cytometry. Results: Aspergillus sensitization was detected in 33 (35.4%) patients with severe BA. In patients with BA and Aspergillus sensitization, the disease course was uncontrolled, and patients in this group were credibly more likely to receive oral glucocorticosteroids. The number of basophils (activated by the A. fumigatus allergen) and the stimulation index in patients with BA and Aspergillus sensitization were significantly higher than in patients with BA (9.9 (6.0–24.0) % vs. 3.6 (2.0–5.4) %; (p=0.000) and 4.25 (2.49–9.30) vs. 0.94 (0.75–1.16); (p=0.000)). Significant differences in TARC content were obtained in the groups of patients with severe BA and Aspergillus sensitization and patients with BA (625.0 (418.4–875.0) pg/mg versus 406.0 (210.0–561.0) pg/mg; p=0.001). A negative correlation was determined between TARC levels and a decrease in forced expiratory volume in 1 second (FEV1) (r=-0.70, p<0.05), and between positive correlation and absolute eosinophil count (r=0.81, p<0.05) and level of specific IgE to Aspergillus (r=0.36, p<0.05). Conclusion: Aspergillus sensitization is associated with an uncontrolled BA course. An additional method for diagnosing mycogenic sensitization is the BAT. The TARC concentration can serve as a biomarker of an active inflammatory response. KEYWORDS: Aspergillus spp., severe bronchial asthma, mycogenic sensitization, basophil activation test, TARC. FOR CITATION: Kozlova Ya.I., Frolova E.V., Uchevatkina A.E. et al. Clinical and immunological characteristics of severe bronchial asthma with Aspergillus sensitization. Russian Medical Inquiry. 2021;5(1):10–16. DOI: 10.32364/2587-6821-2021-5-1-10-16.

scholarly journals A Case of Anaphylaxis in Which a Basophil Activation Test Was Used to Identify the Suspected Agent

2020 ◽  
Vol 67 (3) ◽  
pp. 172-173
Author(s):  
Takashi Goto ◽  
Shintaro Hayashi ◽  
Hiroko Tsugane ◽  
Mitsuo Iinuma ◽  
Satoru Sakurai
Keyword(s):  
Case Report ◽  
Diagnostic Accuracy ◽  
Sensitivity And Specificity ◽  
Skin Testing ◽  
High Sensitivity ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Etiological Agent ◽  
Skin Tests ◽  
Activation Test

This is a case report of anaphylaxis in which the basophil activation test (BAT) was used to identify the etiological agent. Although skin tests are considered the most effective methods for identifying anaphylactic triggers, the test itself presents a risk of inducing anaphylaxis. The BAT is advantageous because of its inherent lack of risk, high sensitivity and specificity to identify the suspected anaphylactic agents, and diagnostic accuracy comparable to conventional skin testing. Therefore, in the future, the BAT is likely to become the preferred test for the detection of allergens over conventional skin tests.

scholarly journals Basophil activation: theoretical aspects and use in the diagnosis of allergic diseases

2021 ◽  
Vol 23 (3) ◽  
pp. 469-482
Author(s):  
N. V. Bychkova
Keyword(s):  
Sensitivity And Specificity ◽  
Allergic Inflammation ◽  
Allergic Diseases ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
Basophil Activation ◽  
Present Stage ◽  
Laboratory Methods ◽  
Activation Test

Diagnostics of allergic diseases is a difficult issue, which requires distinct solutions, since this disorder is very common among the population. The overview focuses on complex diagnostics, including various methods that are most in demand at the present stage. The allergy diagnostics primarily include taking anamnesis, physical examination, instrumental and functional tests. Less often, the provocative tests are used, due to risk of severe adverse reactions. At the present stage, the role of laboratory diagnostics of allergies is growing, since, firstly, there is an increase in difficult-to-diagnose cases that require involvement of the entire medical armamentarium, and, secondly, the sensitivity and specificity of laboratory tests are improving. Among laboratory methods, the most significant are the assessment of the level of specific IgE, and the relatively new basophile activation test. The latter test is the main focus of the present review. It is functional and combines the advantages of provocative tests, during which conditions are created for the interaction of a potential allergen and effector cells of allergic inflammation, keeping safety for the patient. The data on the life cycle of basophils, their expression of membrane receptors, the content of granules, and ability to produce additional inflammatory mediators by the cells are presented. Participation of these cells in pathogenesis of allergic inflammation is being considered. Various mechanisms of basophil activation are discussed, both IgE-mediated and IgE-independent, which are similar in vivo and in vitro. Theoretical aspects of using the in vitro basophil activation test to estimate the hypersensitivity to a wide range of allergens are discussed. High sensitivity and specificity of the test for diagnosing allergies to food, household, pollen, insect and drug allergens are presented. Specific features of the basophil activation test related to the preanalytical, analytical and postanalytical stages of the study are highlighted. The factors influencing evaluation of this method are known. For example, difficulties in interpreting the test may arise while taking glucocorticosteroid hormones, in acute period of inflammation, with severe edema. The possibility of using this test to assess effectiveness of allergen-specific and anti-IgE therapy is being considered. A comparison of the basophil activation test, measurement of specific IgE and skin tests by various parameters related to performance and interpretation of results is carried out. Comprehensive diagnostics of allergic diseases, including usage of pathogenetically determined laboratory methods, will contribute to adequate treatment and, as a result, improve the health of the population.

scholarly journals Basophil activation test in allergy diagnostics

2020 ◽  
Vol 74 ◽  
pp. 548-555
Author(s):  
Karolina Nowakowska ◽  
Emilia Królewicz ◽  
Andrzej Gamian ◽  
Wojciech Barg
Keyword(s):  
Skin Prick Testing ◽  
Allergic Diseases ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
Basophil Activation ◽  
Diagnostic Methods ◽  
Activation Test ◽  
Allergy Diagnostics ◽  
Continue Research ◽  
Laboratory Tool

The prevalence of allergic diseases in Poland and in the world continues to rise in recent years. The most commonly used methods for diagnosing IgE – dependent allergies are skin prick testing (SPT) and assessment of specific IgE (sIgE) directed against specific allergens. However, both methods have some disadvantages and the obtained results may be inconsistent. In particular, routine diagnostic tests are not always effective for some drugs and foods. Consequently, additional laboratory tools should be used. Basophil activation test (BAT) based on flow cytometry is a promising diagnostic method. The present paper demonstrates the usefulness and effectiveness of BAT protocols in allergy diagnosis in scientific research. In comparison to routinely used diagnostic methods, BAT is an expensive and complicated laboratory tool. However, it offers the possibility to efficiently and effectively recognize allergies. Introducing BAT into routine diagnostics in allergology is problematic because this method has not yet been standardized and validated. Therefore, there is a justified need to continue research in this field. If standardized and validated, BAT may offer a reasonable improvement in allergy diagnostics in the future.

scholarly journals New opportunities in the diagnosis of asthma with sensitization to Aspergillus spp.

2021 ◽  
Vol 13 (2) ◽  
pp. 67-76
Author(s):  
Yana I. Kozlova ◽  
Alexandra E. Uchevatkina ◽  
Larisa V. Filippova ◽  
Oleg V. Aak ◽  
Valeriy D. Kuznetsov ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Stimulation Index ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Basophil Activation Test ◽  
Study Groups ◽  
Specific Ige ◽  
Basophil Activation ◽  
Activation Test ◽  
Cellular Analysis ◽  
Index Value

BACKGROUND:Diagnosis of asthma with sensitization toAspergillusspp. is becoming increasingly important due to the severe, uncontrolled course of the disease and the possibility of the formation of allergic bronchopulmonary aspergillosis. AIM:To evaluate the possibility of using the basophil activation test using flow cytometry for the diagnosis of asthma with sensitization toAspergillusspp. MATERIALS AND METHODS:118 patients with asthma were examined. The levels of total IgE and specific IgE to aeroallergens were determined in the blood serum by the enzyme immunoassay. Basophil activation was studied by flow cytometry using the Allergenicity kit (Cellular Analysis of Allergy, Beckman-Coulter, USA). The allergenAspergillus fumigatus(Alcor Bio, Russia) was used to stimulate basophils. RESULTS:The first group consisted of 57 patients with asthma without sensitization toAspergillusspp. The second group included 36 patients with asthma with sensitization toAspergillusspp. The third group consisted of 25 patients with allergic bronchopulmonary aspergillosis. The number of basophils activated by theAspergillusfumigatusallergen in patients with asthma with sensitization toAspergillusspp. and allergic bronchopulmonary aspergillosis was significantly higher than in the asthma group and amounted to 8.1 [5.2; 20.9]% and 84.6 [75.7; 94.0]%, respectively (p 0.001). The stimulation index in the study groups ranged from 0.7 to 72.6. The optimal diagnostic point (cut off) for identifying patients with asthma withAspergillusspp. sensitization there was an stimulation index value of more than 2.4, and for patients with allergic bronchopulmonary aspergillosis 15.95. Among all patients with sensitization toAspergillusspp. a positive correlation was established between the level of specific IgE toAspergillusspp. and the percentage of basophils activated by the allergenAspergillusfumigatus(r= 0.792,p 0.001) and stimulation index (r= 0.796,p 0.05). CONCLUSIONS:The basophil activation test can be used as an additional diagnostic method for asthma with sensitization toAspergillusspp. and allergic bronchopulmonary aspergillosis.

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