scholarly journals Detection of Common Disease-Causing Mutations in Mitochondrial DNA (Mitochondrial Encephalomyopathy, Lactic Acidosis with Stroke-Like Episodes MTTL1 3243 A>G and Myoclonic Epilepsy Associated with Ragged-Red Fibers MTTK 8344A>G) by Real-Time Polymerase Chain Reaction

2006 ◽  
Vol 8 (2) ◽  
pp. 277-281 ◽  
Author(s):  
Hongxin Fan ◽  
Chris Civalier ◽  
Jessica K. Booker ◽  
Margaret L. Gulley ◽  
Thomas W. Prior ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mitochondrial Dna ◽  
Lactic Acidosis ◽  
Real Time ◽  
Myoclonic Epilepsy ◽  
Mitochondrial Encephalomyopathy ◽  
Common Disease ◽  
Chain Reaction ◽  
Ragged Red Fibers ◽  
Polymerase Chain

RETRACTED: Quantification of mitochondrial DNA using real-time polymerase chain reaction in patients with premature ovarian failure

2005 ◽  
Vol 84 (6) ◽  
pp. 1712-1718 ◽  
Author(s):  
Kwang-Yul Cha ◽  
Sook-Hwan Lee ◽  
Hyung-Min Chung ◽  
Kwang-Hyun Baek ◽  
Sung-Won Cho ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mitochondrial Dna ◽  
Real Time ◽  
Premature Ovarian Failure ◽  
Ovarian Failure ◽  
Chain Reaction ◽  
Polymerase Chain

Transovarial transmission of dengue 1 virus in Aedes aegypti larvae: real-time PCR analysis in a Brazilian city with high mosquito population density

2018 ◽  
Vol 64 (6) ◽  
pp. 393-400 ◽  
Author(s):  
Alexsander Moraes ◽  
Filipe C. Cortelli ◽  
Taís B. Miranda ◽  
Davi R. Aquino ◽  
José R. Cortelli ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Real Time ◽  
Pcr Analysis ◽  
Transovarial Transmission ◽  
Common Disease ◽  
Chain Reaction ◽  
Primary Mechanism ◽  
Virus Serotype ◽  
Polymerase Chain

Transovarial transmission is among the reported factors able to influence environmental maintenance of dengue virus (DENV). Endemic areas with active transmission of dengue are suitable for studying transovarial transmission. Brazil is a country where dengue is endemic and where DENV-1 is the most common disease-related virus serotype. This study aimed to identify transovarial transmission of DENV-1 in Aedes aegypti larvae by reverse-transcriptase nested real-time polymerase chain reaction. Between March and October 2016, Culicidae larvae were collected using traps in 3 locations in Taubaté, São Paulo, Brazil, which has a high occurrence of dengue. The collected larvae were sacrificed in the 3rd or 4th larval stage, classified, and stored at –20 °C. The A. aegypti larvae samples (n = 910) were separated into 91 pools of 10 specimens each from which RNA was extracted, reverse transcribed into cDNA, and analyzed by nested qPCR. None of the pools tested positive for DENV-1. Due to the absence of detectable virus in the evaluated samples, we concluded that transovarial transmission may not be the primary mechanism for maintenance of DENV-1 in this particular environment.

Detection of mitochondrial DNA from human inner ear using real-time polymerase chain reaction and laser microdissection

2005 ◽  
Vol 125 (7) ◽  
pp. 697-701 ◽  
Author(s):  
Yurika Kimura ◽  
Hiroko Kouda ◽  
Yoshinobu Eishi ◽  
Daisuke Kobayashi ◽  
Yoshimi Suzuki ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mitochondrial Dna ◽  
Inner Ear ◽  
Real Time ◽  
Laser Microdissection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Human Inner Ear
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