Abstract
The earliest records in Britain for the western house mouse ( Mus musculus domesticus ) date from the Late Bronze Age. As a commensal species, the arrival to Britain is suggested to be related with human transport and trade. In order to study the arrival of the species in Britain, we collected a total of 16 ancient mouse mandibulae from some of the rare early sites with the species in the four British archaeological sites, ranging from the Late Bronze Age to the Roman period. From these, we obtained the first ancient mitochondrial DNA (mtDNA) house mouse sequences from the British Isles, including eight house mice from three different archaeological sites ranging from the Late Bronze Age to the Middle Iron Age. We also obtained five ancient mtDNA wood mouse ( Apodemus spp.) sequences from across all four sites. Modern British house mouse mtDNAs are primarily characterised by haplogroups E, F and, much less commonly, haplogroup D. The presence of haplogroups D and E in our samples provides evidence of an early house mouse colonisation that may be related with Late Bronze Age/Iron Age trade and/or expansions of humans. Our results confirm the hypothesis, based on zooarchaeological evidence and modern mtDNA predictions, that house mice with haplogroups D and E were established in Britain by the Iron Age and, in the case of haplogroup E, possibly even by the Late Bronze Age.