Tubifex tubifex chronic toxicity test using artificial sediment: methodological issues

In this work, the chronic toxicity of a mixture of light-stick chemicals and water was tested. The light-stick is used in fishery activities to catch swordfish. The tubes were collected on the beaches of the Costa dos Coqueiros - BA, Brazil, in the period from 14th to 31st July 2007. The method used was a short chronic toxicity test where embryos of the sea urchins Echinometra lucunter and Lytechinus variegatus were exposed to a stock solution consisting of the supernatant formed from a mixture of sea water and the orange-colored light-stick chemical. After a preliminary test, concentrations defined were 0.002, 0.003, 0.01, 0.02, 0.1, 1.0% of stock solution. The final test ran for 36 hours for E. Lucunter and 24 hours for L. variegatus with 4 replicates for each concentration. The value of EC50 - 36h was 0.062% with confidence limits ranging from 0.042 to 0.079% and the EC50 - 24h was 0.011% with confidence limits ranging from 0.009 to 0.014%, i.e., the chemical mix present in the light-stick is potentially toxic. So, as these flags are commonly used for fishing there is potential danger in their disposal in the open ocean.

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Optimal conditions for three brood chronic toxicity test method using a freshwater macroinvertebrate Moina macrocopa

Environmental Monitoring and Assessment ◽

10.1007/s10661-011-2216-2 ◽

2011 ◽

Vol 184 (6) ◽

pp. 3687-3695 ◽

Cited By ~ 16

Author(s):

Sorin Oh ◽

Kyungho Choi

Keyword(s):

Toxicity Test ◽

Chronic Toxicity ◽

Test Method ◽

Optimal Conditions ◽

Moina Macrocopa ◽

Chronic Toxicity Test

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Evaluation of a static renewal chronic toxicity test method forDaphnia magnastraus using boric acid

Environmental Toxicology and Chemistry ◽

10.1002/etc.5620030111 ◽

1984 ◽

Vol 3 (1) ◽

pp. 89-94 ◽

Cited By ~ 24

Author(s):

Francis M. Gersich

Keyword(s):

Boric Acid ◽

Toxicity Test ◽

Chronic Toxicity ◽

Test Method ◽

Chronic Toxicity Test

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Przykłady wykorzystania testu OSTRACODTOXKIT F™ do oceny zanieczyszczenia pyłów drogowych metalami w aglomeracji wrocławskiej

Przegląd Naukowy Inżynieria i Kształtowanie Środowiska ◽

10.22630/pniks.2020.29.1.3 ◽

2020 ◽

Vol 29 (1) ◽

pp. 27-36

Author(s):

Magdalena Wróbel ◽

Wioletta Rogula-Kozłowska ◽

Justyna Rybak

Keyword(s):

Growth Inhibition ◽

Toxicity Test ◽

Chronic Toxicity ◽

Road Dust ◽

Water Bodies ◽

City Centre ◽

Aquatic Life ◽

Chronic Toxicity Test ◽

Living Organisms ◽

The Impact

Pollutants present in road dust deriving from traffic, such as PAHs and other organic compounds or heavy metals, are washed out with rain and get into the water bodies accumulating in sediments for many years and simultaneously posing a threat to aquatic life and significantly affecting water quality. To study the impact of these toxic compounds on the health of living organisms, routine tests of water and sediments chemistry are insufficient as studies based on living organisms are much more reliable. And therefore, in order to know the response of living organisms to road dust pollutants which enter the water bodies the chronic toxicity test OSTRACODTOXKIT F™ was used. This test is based on the observation of development of Heterocypris incongruens that normally lives in sediments. Ostracod, H. incongruens is very sensitive to heavy metal contamination, thus it is a very good tool to study toxicity of road dust washed out with rain into the water bodies. The research was conducted in the Wrocław agglomeration (in the city centre and suburbs) at sites differing in the intensity of car traffic. We observed that road dust had a significant effect on growth inhibition and death of ostracods, as highest growth inhibition and mortality in the busy areas occurred which also corresponded with highest concentrations of studied elements at these sites. On the other hand, road dust collected in the suburbs of Wrocław did not cause death of H. incongruens and only slightly affected their development. In conclusion, we can state that the chronic toxicity test OSTRACODTOXKIT F™ is a suitable tool to study the impact of road dust on the aquatic ecosystem.

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