Toxicity Study of 1-(2, 5-Dihidroxyphenil)-3-Pyridine-2-Il-Propenone In Adult Female Mice

This research aimed to evaluate the toxicity of 1-(2, 5-dihidroxyphenil)-3-pyridine-2-il-propenone (DPP) after 24 hours and 90-day administration in female mice. Acute toxicity test was performed using the OECD 423 method, and DPP was administered once a day at doses of 300, 2000, and 5000 mg/kg body weight (BW). Toxic symptoms were observed after 24 hours of administration, and this continued until the 14th day. The experimental animals were dissected and examined for histological organs on the 15th day. The sub-chronic toxicity test was performed using the OECD 408 method, and DPP at 14, 28, and 56 mg/kg/day was administered for 90 days. Toxic symptoms were observed every day, and the amount of food and water intakes were also measured. Furthermore, statistical analysis was performed, and changes in body weight as well as routine blood checks and biochemistry were observed. At the end of the study, experimental animals were killed and the vital organs' weights were examined before their histological analysis. The results showed that DPP at 300-5000 mg/kg/day and 14-56 mg/kg/day for 90 days did not show any toxic symptom respectively. In the sub-chronic toxicity test, no change was observed in blood and urine biochemical parameters (p≥0.05). However, lymphocytic infiltration in the liver and congested vessel in the kidney occurred after administration at 56 mg/kg / day. The results showed that the acute toxicity of DPP is at category 5 according to Globally Harmonized Classification System and sub-chronic toxicity is at a dose below 56 mg/kg/day.

Przykłady wykorzystania testu OSTRACODTOXKIT F™ do oceny zanieczyszczenia pyłów drogowych metalami w aglomeracji wrocławskiej

Pollutants present in road dust deriving from traffic, such as PAHs and other organic compounds or heavy metals, are washed out with rain and get into the water bodies accumulating in sediments for many years and simultaneously posing a threat to aquatic life and significantly affecting water quality. To study the impact of these toxic compounds on the health of living organisms, routine tests of water and sediments chemistry are insufficient as studies based on living organisms are much more reliable. And therefore, in order to know the response of living organisms to road dust pollutants which enter the water bodies the chronic toxicity test OSTRACODTOXKIT F™ was used. This test is based on the observation of development of Heterocypris incongruens that normally lives in sediments. Ostracod, H. incongruens is very sensitive to heavy metal contamination, thus it is a very good tool to study toxicity of road dust washed out with rain into the water bodies. The research was conducted in the Wrocław agglomeration (in the city centre and suburbs) at sites differing in the intensity of car traffic. We observed that road dust had a significant effect on growth inhibition and death of ostracods, as highest growth inhibition and mortality in the busy areas occurred which also corresponded with highest concentrations of studied elements at these sites. On the other hand, road dust collected in the suburbs of Wrocław did not cause death of H. incongruens and only slightly affected their development. In conclusion, we can state that the chronic toxicity test OSTRACODTOXKIT F™ is a suitable tool to study the impact of road dust on the aquatic ecosystem.