Bone marrow stem cells for the critical limb ischemia treatment: biological aspects and clinical application

2018 ◽  
Vol XIII (1) ◽  
Author(s):  
P. Orekhov ◽  
M. Konoplyannikov ◽  
V. Baklaushev ◽  
V. Kalsin ◽  
A. Averyanov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Clinical Application ◽  
Critical Limb Ischemia ◽  
Limb Ischemia ◽  
Bone Marrow Stem Cells ◽  
Biological Aspects

scholarly journals A Phase II Trial of Autologous Transplantation of Bone Marrow Stem Cells for Critical Limb Ischemia: Results of the Naples and Pietra Ligure Evaluation of Stem Cells Study

2012 ◽  
Vol 1 (7) ◽  
pp. 572-578 ◽  
Author(s):  
Alessandro Schiavetta ◽  
Ciro Maione ◽  
Chiara Botti ◽  
Gennaro Marino ◽  
Stefano Lillo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Phase Ii ◽  
Critical Limb Ischemia ◽  
Phase Ii Trial ◽  
Autologous Transplantation ◽  
Limb Ischemia ◽  
Bone Marrow Stem Cells

scholarly journals Comparative analysis of mouse bone marrow and adipose tissue mesenchymal stem cells for critical limb ischemia cell therapy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Pegah Nammian ◽  
Seyedeh-Leili Asadi-Yousefabad ◽  
Sajad Daneshi ◽  
Mohammad Hasan Sheikhha ◽  
Seyed Mohammad Bagher Tabei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cell Therapy ◽  
Femoral Artery ◽  
Critical Limb Ischemia ◽  
Limb Ischemia

Abstract Introduction Critical limb ischemia (CLI) is the most advanced form of peripheral arterial disease (PAD) characterized by ischemic rest pain and non-healing ulcers. Currently, the standard therapy for CLI is the surgical reconstruction and endovascular therapy or limb amputation for patients with no treatment options. Neovasculogenesis induced by mesenchymal stem cells (MSCs) therapy is a promising approach to improve CLI. Owing to their angiogenic and immunomodulatory potential, MSCs are perfect candidates for the treatment of CLI. The purpose of this study was to determine and compare the in vitro and in vivo effects of allogeneic bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue mesenchymal stem cells (AT-MSCs) on CLI treatment. Methods For the first step, BM-MSCs and AT-MSCs were isolated and characterized for the characteristic MSC phenotypes. Then, femoral artery ligation and total excision of the femoral artery were performed on C57BL/6 mice to create a CLI model. The cells were evaluated for their in vitro and in vivo biological characteristics for CLI cell therapy. In order to determine these characteristics, the following tests were performed: morphology, flow cytometry, differentiation to osteocyte and adipocyte, wound healing assay, and behavioral tests including Tarlov, Ischemia, Modified ischemia, Function and the grade of limb necrosis scores, donor cell survival assay, and histological analysis. Results Our cellular and functional tests indicated that during 28 days after cell transplantation, BM-MSCs had a great effect on endothelial cell migration, muscle restructure, functional improvements, and neovascularization in ischemic tissues compared with AT-MSCs and control groups. Conclusions Allogeneic BM-MSC transplantation resulted in a more effective recovery from critical limb ischemia compared to AT-MSCs transplantation. In fact, BM-MSC transplantation could be considered as a promising therapy for diseases with insufficient angiogenesis including hindlimb ischemia.

Abstract No. 175: Endovascular Treatment of Critical Limb Ischemia by Autologous Bone-Marrow Derived Stem Cells

2008 ◽  
Vol 19 (2) ◽  
pp. S67
Author(s):  
S. Sharma ◽  
G.S. Gulati ◽  
P. Jagia ◽  
V.K. Sheorain ◽  
S. Mohanty
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Endovascular Treatment ◽  
Critical Limb Ischemia ◽  
Autologous Bone Marrow ◽  
Limb Ischemia ◽  
Autologous Bone

scholarly journals Increasing injection frequency enhances the survival of injected bone marrow derived mesenchymal stem cells in a critical limb ischemia animal model

2016 ◽  
Vol 20 (6) ◽  
pp. 657 ◽  
Author(s):  
Woong Chol Kang ◽  
Pyung Chun Oh ◽  
Kyounghoon Lee ◽  
Taehoon Ahn ◽  
Kyunghee Byun
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Animal Model ◽  
Mesenchymal Stem Cells ◽  
Critical Limb Ischemia ◽  
Limb Ischemia ◽  
Injection Frequency

scholarly journals Characterization of Mesenchymal Stem Cells of “No-Options” Patients with Critical Limb Ischemia Treated by Autologous Bone Marrow Mononuclear Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e73722 ◽  
Author(s):  
Cestmir Altaner ◽  
Veronika Altanerova ◽  
Marina Cihova ◽  
Lubica Hunakova ◽  
Katarina Kaiserova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Critical Limb Ischemia ◽  
Mononuclear Cells ◽  
Autologous Bone Marrow ◽  
Limb Ischemia ◽  
Bone Marrow Mononuclear Cells ◽  
Autologous Bone

Bone Marrow Stem Cells in Clinical Application: Harnessing Paracrine Roles and Niche Mechanisms

2010 ◽  
pp. 265-292 ◽  
Author(s):  
Rania M. El Backly ◽  
Ranieri Cancedda
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Clinical Application ◽  
Bone Marrow Stem Cells

scholarly journals Early Results of Clinical Application of Autologous Whole Bone Marrow Stem Cell Transplantation for Critical Limb Ischemia with Buerger’s Disease

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Seon-Hee Heo ◽  
Yoong-Seok Park ◽  
Eun-Suk Kang ◽  
Kwang-Bo Park ◽  
Young-Soo Do ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Clinical Application ◽  
Critical Limb Ischemia ◽  
Limb Ischemia ◽  
Bone Marrow Stem Cell ◽  
Early Results ◽  
Marrow Stem Cell

Abstract 231: Bone Marrow-Derived Mesenchymal Stem Cells from Amputated Limbs of Patients with Critical Limb Ischemia Provide Stromal Support for Endothelial Cells

2013 ◽  
Vol 33 (suppl_1) ◽  
Author(s):  
Ruoya Wang ◽  
Ian Copland ◽  
Haiyan Li ◽  
Alexandra Peister ◽  
Todd McDevitt ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Critical Limb Ischemia ◽  
Healthy Donor ◽  
Cellular Therapy ◽  
Limb Ischemia ◽  
Growth Media ◽  
Stromal Support ◽  
The Difference

Critical limb ischemia (CLI) often results in amputation, but autologous cellular therapy using bone marrow-derived mesenchymal stem cells (MSCs) may be able to prevent this outcome. However, the quality of MSCs in the CLI population is not clear. Amputated CLI limbs contain reservoirs of MSCs that can be used to characterize their stromal capacity. We hypothesize that MSC stromal capacity is relatively preserved in ischemic limbs compared to ones from healthy donors. Methods Healthy donor MSCs (hdMSCs, n=2) and ischemic MSCs (iMSCs, n=3) from amputated limbs of non-diabetic CLI patients were cultured. Human microdermal ECs were commercially obtained. Co-culture pellets (1:1 ratio) of hdMSC:EC, iMSC:EC, and EC alone were formed and cultured in 3-D fibrin hydrogels in EC growth media. Images of the pellets and invasion areas were captured up to 5 days. The invasion area was quantified as the difference between the total and pellet area normalized to initial pellet area. Experiments were performed in quadruplicates and co-culture experiments were duplicated. Results iMSC:EC co-culture provided similar stromal support as the healthy donor cohort. Both MSC groups were significantly greater than the ECs alone following day 2 (see figure, *P<0.0005, ANOVA). Both MSC groups also exhibited significant increase in invasion area with time (P<0.01) while the ECs did not (P>0.5). Conclusion This is the first study to demonstrate that iMSCs provide similar stromal support to ECs as healthy hdMSCs, thus iMSCs may be of sufficient quality for use in autologous therapy. We are currently quantifying secretome expressions of the iMSCs to provide insights into mechanisms of the stromal support.

Bone marrow stem cells make hepatocytes with replicative potential

2001 ◽  
Vol 120 (5) ◽  
pp. A62-A62
Author(s):  
S FORBES ◽  
M ALISON ◽  
K HODIVALADILKE ◽  
R JEFFERY ◽  
R POULSOM ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Stem Cells
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