scholarly journals Symptom expression and accumulation of potato virus Y (PVYO) and potato leaf roll virus in thirteen potato cultivars

1993 ◽  
Vol 2 (1) ◽  
pp. 33-40
Author(s):  
Jari Valkonen ◽  
Eerik Mäkäräinen
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Secondary Infection ◽  
Leaf Roll ◽  
Symptom Expression ◽  
Potato Leaf ◽  
Systemic Necrosis ◽  
Local Lesions ◽  
Ordinary Strain ◽  
Leafroll Virus

Necrotic local lesions developed in cvs. Matilda, Ostara, Record, Satuma, Stina, Hankkija’s (Hjan) Tanu and Hjan Timo and local ring spots in Olympia and Sieglinde (Siikli) following sap inoculation with the ordinary strain of potato virus Y (PVY0). Secondarily infected cvs. Ostara, Pito, Siikli and Hjan Timo developed leaf drop. No infected progeny was produced by Matilda, Saturna and Hjan Tanu. In contrast, Bintje, Puikula and Sabina developed neither local lesions nor systemic necrosis, but showed mosaic symptoms following primary and secondary infection by PVYO. The ELISA absorbance values for potato leafroll virus (PLRV) in Ostara, Pito and Saturna were less than 10% of those in the PLRV-infected Siikli. The ELISA values for PLRV in Olympia, Stina, Hjan Tanu and Hjan Timo were not significantly different from those of Siikli. The severity of the symptoms did not correlate with the concentration of PLRV in the potatoes.

scholarly journals Strain-Specific Hypersensitive and Extreme Resistance Phenotypes Elicited by Potato virus Y Among 39 Potato Cultivars Released in Three World Regions Over a 117-Year Period

Plant Disease ◽  
2018 ◽  
Vol 102 (1) ◽  
pp. 185-196 ◽  
Author(s):  
Roger A. C. Jones ◽  
Stuart J. Vincent
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Secondary Infection ◽  
Potato Cultivars ◽  
Symptom Expression ◽  
Extreme Resistance ◽  
Potato Plants ◽  
Pvy Resistance ◽  
World Regions ◽  
Healthy Seed

Strain-specific hypersensitive (HR) and extreme resistance (ER) phenotypes elicited in potato plants by three Potato virus Y (PVY) isolates in strain groups PVYO (BL and DEL3) and PVYD (KIP1) were studied. PVYO and PVYD isolates elicit HR genes Ny or putative Nd, respectively, and all three isolates elicit ER gene Ry. They were inoculated to 39 Australasian, European, or North American potato cultivars released over a 117-year period and harvested tubers were replanted. Both primary and secondary symptoms were recorded. Two European cultivars always developed ER following sap and graft inoculation and, thus, carried comprehensive PVY resistance gene Ry. One Australasian and two European cultivars always developed susceptible phenotypes and, thus, lacked genes Ry, Ny, and putative Nd. Sap inoculation with isolate KIP1 elicited localized HR (LHR) in 31 cultivars and both LHR and systemic HR (SHR) in three others; thus, all carried putative Nd. Isolates BL and DEL3 both elicited susceptible phenotypes in 11 of these 34 cultivars but LHR alone, SHR alone, or both LHR and SHR in the other 23 which, therefore, all carry Ny. With these two isolates, SHR expression ranged from very severe to very weak, with the greatest numbers of isolate–cultivar combinations occurring in the severe category with BL (n = 11) and moderate category (n = 12) with DEL3. Within the same isolate–cultivar combination, overall, SHR symptom expression was weaker with secondary than primary infection. With both primary and secondary infection, SHR expression was most severe with KIP1 and weakest with DEL3. Genes Ny and putative Nd were present in cultivars released between 1939 and 2010 or 1893 and 2010, respectively, occurring in cultivars from all three world regions. These findings have important implications concerning breeding new PVY-resistant potato cultivars, especially for countries lacking healthy seed potato stocks, or where subsistence farmers cannot afford them. An alternative to including gene Ry is incorporating as many strain-specific PVY resistance genes as possible.

scholarly journals Eliminating Potato Virus Y (PVY) and Potato Leaf Roll Virus (PLRV) Using Cryotherapy of in vitro-grown Potato Shoot Tips

2014 ◽  
Vol 59 (4) ◽  
pp. 498-504 ◽  
Author(s):  
Jung-Yoon Yi ◽  
◽  
Gi-An Lee ◽  
Jong-Wook Jeong ◽  
Sok-Young Lee ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Leaf Roll ◽  
Shoot Tips ◽  
Potato Leaf Roll Virus ◽  
Potato Leaf

scholarly journals Field Evaluation and Enzyme-Linked Immunosorbent Assay Detection of Potato Leaf Roll Virus, Potato Virus X and Potato Virus Y in Potato Germplasm

2017 ◽  
Vol 9 (7) ◽  
pp. 229 ◽  
Author(s):  
Romana Anjum ◽  
M. Aslam Khan ◽  
Kolawole Oluwaseun Olawale ◽  
Raheel Baber
Keyword(s):  
Immunosorbent Assay ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Rating Scale ◽  
Leaf Roll ◽  
Potato Virus X ◽  
Potato Leaf Roll Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Potato Leaf ◽  
Potato Varieties

Polerovirus: potato leaf roll virus (PLRV), Potyvirus: potato virus Y (PVY) and Potexvirus: potato virus X (PVX) is more destructive and well distributed throughout the Pakistan. Incidence has been reported to be as high as 90%, 25%, and ≥ 15%, respectively in the potato growing regions. To find out the source of resistance, twenty-nine virus free potato varieties were grown under field conditions with good agricultural practices. The disease severity of PLRV, PVY and PVX was recorded to determine the level of resistance of the potato varieties according to the disease rating scale. Infectivity and biological assay of all twenty-nine varieties were done in green house on potato, Datura stramonium, Nicotiana glutinosa and Physalis floridana. Non-inoculated plants were served as control. Leaf samples from potato varieties were collected for serological detection of PLRV, PVY and PVX by Double antibody sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). Out of twenty nine varieties, none of the variety was resistant to PLRV although three varieties; Mirrato, 394021-120 and Orla were moderately resistant. Only FD 48-4 and TPS 9813 showed resistance to PVX and PVY. While FD 3-10, FD 9616 and FD 37-13 were moderately to PVX and PVY. Rest of the varieties was found susceptible to all three viruses.

scholarly journals ELISA reagents for potato virus Y strains with significantly low non-specific reactions

1984 ◽  
Vol 56 (1) ◽  
pp. 89-95
Author(s):  
Aarne Kurppa ◽  
Kirsi Korhonen
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Secondary Infection ◽  
Protein A ◽  
Elisa Test ◽  
Leaf Sample ◽  
Potato Leaf ◽  
Specific Antisera ◽  
Specific Reactions

High titered and highly virus-specific antisera to selected PVYo and PVYn antigens and their mixture were produced in rabbits. Immunoglobulins purified from the antisera with the protein A method and their enzyme conjugates had very strong virus-specific but no non-specific reactions in the ELISA test. Their homologous reactions to the antigens were stronger than heterologous but any PVY isolate could be identified in a potato leaf sample with the dilutions between 10-2 and 10-3 and in a sample from sprouted tubers with secondary infection diluted between 10-1 and 10-2.

scholarly journals Response of Potato Cultivars to Five Isolates Belonging to Four Strains of Potato virus Y

Plant Disease ◽  
2012 ◽  
Vol 96 (10) ◽  
pp. 1422-1429 ◽  
Author(s):  
Bihua Nie ◽  
Mathuresh Singh ◽  
Agnes Murphy ◽  
Andrew Sullivan ◽  
Conghua Xie ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Secondary Infection ◽  
Potato Cultivar ◽  
Potato Cultivars ◽  
Systemic Necrosis ◽  
Ranger Russet ◽  
Russet Norkotah ◽  
Secondary Infections ◽  
Infection Experiments

The responses of 14 potato cultivars to five Potato virus Y (PVY) isolates belonging to four strains (ordinary [PVYO], tobacco veinal necrosis [PVYN], N:O group [PVYN:O], and nonrecombinant potato tuber necrotic [PVYNTN]) were studied in primary and secondary infections. For the primary infection experiments, foliage symptoms were monitored daily after mechanical inoculation with a PVY isolate until harvest; and, for the secondary infection experiments, foliage symptoms were monitored regularly from plant emergence until harvest. Tuber symptoms (namely, tuber necrotic ringspots) were checked at harvest and monthly postharvest for up to 4 months. In both infections, symptoms varied significantly depending on potato cultivar and virus strain or isolate. In primary infections, local lesions occurred on inoculated leaves of ‘AC Chaleur’, ‘Eramosa’, ‘Goldrush’, ‘Jemseg’, ‘Katahdin’, ‘Ranger Russet’, and ‘Yukon Gold’ after inoculation with PVYO isolates, followed by systemic necrosis on latterly emerged uninoculated leaves. In contrast, plants of ‘CalWhite’, ‘La Rouge’, ‘Red LaSoda’, ‘Russet Burbank’, ‘Russet Norkotah’, and ‘Superior’ did not exhibit any visible symptoms on inoculated leaves but developed mild to severe mosaic on latterly emerged leaves after infection with PVYO isolates. In all cultivars, near-symptomless to mild mosaic was induced by PVYN and mild to severe mosaic by PVYN:O. PVYNTN induced mild to severe mosaic in plants of all cultivars except AC Chaleur, ‘Cherokee’, and Yukon Gold, which developed visible systemic necrosis. Necrotic ringspots were observed in tubers of PVYNTN-infected plants of AC Chaleur, Cherokee, and Yukon Gold. The tuber symptoms were also incited by PVYN-Jg on Cherokee. In secondary infections, the symptoms were generally more severe than primary infections even though the symptom types did not alter. As in the greenhouse, a clear symptom severity pattern (PVYO-FL > PVYO-RB > PVYNTN-Sl > PVYN:O-Mb58 > PVYN-Jg) was observed in AC Chaleur, Cherokee, Eramosa, Goldrush, Jemseg, Katahdin, Ranger Russet, and Yukon Gold in the field.

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