scholarly journals IDENTIFICATION AND CHARACTERIZATION OF RHIZOSPHERIC MICROBIAL ISOLATES WITH ANTIMICROBIAL (ANTIFUNGAL) ACTIVITY FROM SORGASTRUM NUTANS

2016 ◽  
Vol 2 (4) ◽  
Author(s):  
MRRIDULA DANGI NARWAL
Keyword(s):  
Plant Growth ◽  
Hydrogen Cyanide ◽  
Phosphate Solubilization ◽  
Acc Deaminase ◽  
Antagonistic Action ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Identification And Characterization ◽  
Microbial Isolates

The goal of this review was to disconnect and describe a rhizospheric bacterium from Sorgastrum nutans, developing around the betray district of Rajasthan (India). Plant growth promoting rhizobacterias (PGPR) are known to impact plant development by different immediate or circuitous systems. Separated strain was tried for different PGP characteristics like 1-aminocyclopropane-1-carboxylate (ACC) deaminase movement, phosphate solubilization, indole acidic corrosive generation, creation of siderophore, nitrogen obsession and alkali generation. Bio-control capacity of detach was screened by antagonistic action against certain parasitic/bacterial pathogens and additionally Hydrogen cyanide( HCN) creation. Selected test strain was likewise biochemically characterized. Advance recognizable proof of identification was performed by PCR based 16S rRNA qualitative sequencing. Besides assessment of the segregate SNP-18 showing different plant growth promoting (PGP) qualities on soil/plant framework is on-going to reveal their viability as successful PGPR.

scholarly journals Exploring the Multi-trait Plant Growth Promotion Capability of Commercial Liquid Biofertilizers Isolates

2015 ◽  
Vol 9 (3) ◽  
pp. 24-37 ◽  
Author(s):  
Mohammed Faisal Ansari ◽  
Devayani R. Tipre ◽  
Shailesh R. Dave
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Multiple Traits ◽  
The Past ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Field Efficiency

Organic farming is gaining popularity where bio-inoculants could play a key role in promoting the growth of plants. The liquid biofertilizers concept is new to farmers and developed recently. Lots of liquid biofertilizers formulations and field efficiency were shown in the past by various researchers, but the plant growth promoting (PGP) efficiency of the liquid biofertilizers isolates were not reported till date. In the present work 6 different commercially available liquid biofertilizers were used to isolate the organism. These isolated cultures were used to study their PGP efficiency with respect to phosphate solubilization and production of EPS, IAA, siderophore, ammonia, chitinase, ACC-deaminase and HCN. The phosphate solubilization was shown up to 303 g/ml by APS isolate. EPS production was shown by using different C sources and production up to 24 g/l was shown by studied isolated. Most of the organisms studied were able to produce IAA and highest production was shown up to 20 g/ml. More than 65% studied isolates showed siderophore and ACC-deaminase production. The present study shows that the commercial liquid biofertilizer isolates possess multiple traits of plant growth promotion. DOI: http://dx.doi.org/10.3126/ijls.v9i3.12463   International Journal of Life Sciences 9 (3): 2015; 24-37

scholarly journals Characterization of Arsenic-Resistant Endophytic Bacteria From Alfalfa and Chickpea Plants

2021 ◽  
Vol 12 ◽  
Author(s):  
Hazhir Tashan ◽  
Behrouz Harighi ◽  
Jalal Rostamzadeh ◽  
Abdolbaset Azizi
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Lipase Production ◽  
Growth Potential ◽  
Sodium Arsenate ◽  
Plant Growth Promoting ◽  
Growth Promoting

The present investigation was carried out to isolate arsenic (As)-resistant endophytic bacteria from the roots of alfalfa and chickpea plants grown in arsenic-contamination soil, characterize their As tolerance ability, plant growth-promoting characteristics, and their role to induce As resistance by the plant. A total of four root endophytic bacteria were isolated from plants grown in As-contaminated soil (160–260-mg As kg−1 of soil). These isolates were studied for plant growth-promoting (PGP) characteristics through siderophore, phosphate solubilization, nitrogen fixation, protease, and lipase production, and the presence of the arsenate reductase (arsC) gene. Based on 16S rDNA sequence analysis, these isolates belong to the genera Acinetobacter, Pseudomonas, and Rahnella. All isolates were found As tolerant, of which one isolate, Pseudomonas sp. QNC1, showed the highest tolerance up to 350-mM concentration in the LB medium. All isolates exhibited phosphate solubilization activity. Siderophore production activity was shown by only Pseudomonas sp. QNC1, while nitrogen fixation activity was shown by only Rahnella sp. QNC2 isolate. Acinetobacter sp. QNA1, QNA2, and Rahnella sp. QNC2 exhibited lipase production, while only Pseudomonas sp. QNC1 was able to produce protease. The presence of the arsC gene was detected in all isolates. The effect of endophytic bacteria on biomass production of alfalfa and chickpea in five levels of arsenic concentrations (0-, 10-, 50-, 75-, and 100-mg kg−1 soil) was evaluated. The fresh and dry weights of roots of alfalfa and chickpea plants were decreased as the arsenic concentration of the soil was increased. Results indicate that the fresh and dry root weights of alfalfa and chickpea plants were significantly higher in endophytic bacteria-treated plants compared with non-treated plants. Inoculation of chickpea plants with Pseudomonas sp. QNC1 and Rahnella sp. QNC2 induced lower NPR3 gene expression in chickpea roots grown in soil with the final concentration of 100-mg kg−1 sodium arsenate compared with the non-endophyte-treated control. The same results were obtained in Acinetobacter sp. QNA2-treated alfalfa plants grown in the soil plus 50-mg kg−1 sodium arsenate. These results demonstrated that arsenic-resistant endophytic bacteria are potential candidates to enhance plant-growth promotion in As contamination soils. Characterization of bacterial endophytes with plant growth potential can help us apply them to improve plant yield under stress conditions.

Isolation and Characterization of ACC Deaminase Gene from Two Plant Growth-Promoting Rhizobacteria

2008 ◽  
Vol 57 (4) ◽  
pp. 312-317 ◽  
Author(s):  
Venkadasamy Govindasamy ◽  
Murugesan Senthilkumar ◽  
Kishore Gaikwad ◽  
Kannepalli Annapurna
Keyword(s):  
Plant Growth ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

Isolation, selection, and characterization of beneficial rhizobacteria from pea, lentil, and chickpea grown in western Canada

2008 ◽  
Vol 54 (4) ◽  
pp. 248-258 ◽  
Author(s):  
Russell K. Hynes ◽  
Grant C.Y. Leung ◽  
Danielle L.M. Hirkala ◽  
Louise M. Nelson
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Fungal Pathogens ◽  
Crop Production ◽  
Growth Promotion ◽  
Profile Analysis ◽  
Acc Deaminase ◽  
Plant Growth Promoting ◽  
Growth Promoting

The use of beneficial soil microorganisms as agricultural inputs for improved crop production requires selection of rhizosphere-competent microorganisms with plant growth-promoting attributes. A collection of 563 bacteria originating from the roots of pea, lentil, and chickpea grown in Saskatchewan was screened for several plant growth-promoting traits, for suppression of legume fungal pathogens, and for plant growth promotion. Siderophore production was detected in 427 isolates (76%), amino-cyclopropane-1-carboxylic acid (ACC) deaminase activity in 29 isolates (5%), and indole production in 38 isolates (7%). Twenty-six isolates (5%) suppressed the growth of Pythium sp. strain p88-p3, 40 isolates (7%) suppressed the growth of Fusarium avenaceum , and 53 isolates (9%) suppressed the growth of Rhizoctonia solani CKP7. Seventeen isolates (3%) promoted canola root elongation in a growth pouch assay, and of these, 4 isolates promoted the growth of lentil and one isolate promoted the growth of pea. Fatty acid profile analysis and 16S rRNA sequencing of smaller subsets of the isolates that were positive for the plant growth-promotion traits tested showed that 39%–42% were members of the Pseudomonadaceae and 36%–42% of the Enterobacteriaceae families. Several of these isolates may have potential for development as biofertilizers or biopesticides for western Canadian legume crops.

scholarly journals Characterization of ACC deaminase from the biocontrol and plant growth-promoting agent Trichoderma asperellum T203

2010 ◽  
Vol 305 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Ada Viterbo ◽  
Udi Landau ◽  
Sofia Kim ◽  
Leonid Chernin ◽  
Ilan Chet
Keyword(s):  
Plant Growth ◽  
Acc Deaminase ◽  
Trichoderma Asperellum ◽  
Plant Growth Promoting ◽  
Growth Promoting
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