Isolation, selection, and characterization of beneficial rhizobacteria from pea, lentil, and chickpea grown in western Canada

2008 ◽  
Vol 54 (4) ◽  
pp. 248-258 ◽  
Author(s):  
Russell K. Hynes ◽  
Grant C.Y. Leung ◽  
Danielle L.M. Hirkala ◽  
Louise M. Nelson
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Fungal Pathogens ◽  
Crop Production ◽  
Growth Promotion ◽  
Profile Analysis ◽  
Acc Deaminase ◽  
Plant Growth Promoting ◽  
Growth Promoting

The use of beneficial soil microorganisms as agricultural inputs for improved crop production requires selection of rhizosphere-competent microorganisms with plant growth-promoting attributes. A collection of 563 bacteria originating from the roots of pea, lentil, and chickpea grown in Saskatchewan was screened for several plant growth-promoting traits, for suppression of legume fungal pathogens, and for plant growth promotion. Siderophore production was detected in 427 isolates (76%), amino-cyclopropane-1-carboxylic acid (ACC) deaminase activity in 29 isolates (5%), and indole production in 38 isolates (7%). Twenty-six isolates (5%) suppressed the growth of Pythium sp. strain p88-p3, 40 isolates (7%) suppressed the growth of Fusarium avenaceum , and 53 isolates (9%) suppressed the growth of Rhizoctonia solani CKP7. Seventeen isolates (3%) promoted canola root elongation in a growth pouch assay, and of these, 4 isolates promoted the growth of lentil and one isolate promoted the growth of pea. Fatty acid profile analysis and 16S rRNA sequencing of smaller subsets of the isolates that were positive for the plant growth-promotion traits tested showed that 39%–42% were members of the Pseudomonadaceae and 36%–42% of the Enterobacteriaceae families. Several of these isolates may have potential for development as biofertilizers or biopesticides for western Canadian legume crops.

scholarly journals Exploring the Multi-trait Plant Growth Promotion Capability of Commercial Liquid Biofertilizers Isolates

2015 ◽  
Vol 9 (3) ◽  
pp. 24-37 ◽  
Author(s):  
Mohammed Faisal Ansari ◽  
Devayani R. Tipre ◽  
Shailesh R. Dave
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Multiple Traits ◽  
The Past ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Field Efficiency

Organic farming is gaining popularity where bio-inoculants could play a key role in promoting the growth of plants. The liquid biofertilizers concept is new to farmers and developed recently. Lots of liquid biofertilizers formulations and field efficiency were shown in the past by various researchers, but the plant growth promoting (PGP) efficiency of the liquid biofertilizers isolates were not reported till date. In the present work 6 different commercially available liquid biofertilizers were used to isolate the organism. These isolated cultures were used to study their PGP efficiency with respect to phosphate solubilization and production of EPS, IAA, siderophore, ammonia, chitinase, ACC-deaminase and HCN. The phosphate solubilization was shown up to 303 g/ml by APS isolate. EPS production was shown by using different C sources and production up to 24 g/l was shown by studied isolated. Most of the organisms studied were able to produce IAA and highest production was shown up to 20 g/ml. More than 65% studied isolates showed siderophore and ACC-deaminase production. The present study shows that the commercial liquid biofertilizer isolates possess multiple traits of plant growth promotion. DOI: http://dx.doi.org/10.3126/ijls.v9i3.12463   International Journal of Life Sciences 9 (3): 2015; 24-37

scholarly journals Characterization of Selected Plant Growth-Promoting Rhizobacteria and Their Non-Host Growth Promotion Effects

2021 ◽  
Author(s):  
Di Fan ◽  
Donald L. Smith
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Content Type ◽  
Host Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting

There are pressing needs to reduce the use of agrochemicals, and PGPR are receiving increasing interest in plant growth promotion and disease protection. This study follows up our previous report that the four newly isolated rhizobacteria promote the growth of Arabidopsis thaliana .

scholarly journals Silencing of Phytopathogen Communication by the Halotolerant PGPR Staphylococcus Equorum Strain EN21

2019 ◽  
Vol 8 (1) ◽  
pp. 42
Author(s):  
Clara Vega ◽  
Miguel Rodríguez ◽  
Inmaculada Llamas ◽  
Victoria Béjar ◽  
Inmaculada Sampedro
Keyword(s):  
Plant Growth ◽  
Pseudomonas Syringae ◽  
Plant Growth Promotion ◽  
Crop Production ◽  
Growth Promotion ◽  
Signal Molecules ◽  
Staphylococcus Equorum ◽  
Plant Growth Promoting ◽  
Growth Promoting

Increasing world food demand together with soil erosion and indiscriminate use of chemical fertilization highlight the need to adopt sustainable crop production strategies. In this context, a combination of plant growth-promoting rhizobacteria (PGPR) and pathogen management represents a sustainable and efficient alternative. Though little studied, halophilic and halotolerant PGPR could be a beneficial plant growth promotion strategy for saline and non-saline soils. The virulence of many bacterial phytopathogens is regulated by quorum sensing (QS) systems. Quorum quenching (QQ) involves the enzymatic degradation of phytopathogen-generated signal molecules, mainly N-acyl homoserine lactones (AHLs). In this study, we investigate plant growth-promoting (PGP) activity and the capacity of the halotolerant bacterium Staphylococcus equorum strain EN21 to attenuate phytopathogens virulence through QQ. We used biopriming and in vivo tomato plant experiments to analyse the PGP activity of strain EN21. AHL inactivation was observed to reduce Pseudomonas syringae pv. tomato infections in tomato and Arabidopsis plants. Our study of Dickeya solani, Pectobacterium carotovorum subsp. carotovorum and Erwinia amylovora bacteria in potato tubers, carrots and pears, respectively, also demonstrated the effectiveness of QS interruption by EN21. Overall, this study highlights the potential of strain S. equorum EN21 in plant growth promotion and QQ-driven bacterial phytopathogen biocontrol.

scholarly journals Complete Genome Sequence of the Plant Growth-Promoting Bacterium Hartmannibacter diazotrophicus Strain E19T

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Christian Suarez ◽  
Stefan Ratering ◽  
Torsten Hain ◽  
Moritz Fritzenwanker ◽  
Alexander Goesmann ◽  
...  
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Genome Sequence ◽  
Plant Growth Promotion ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Plant Growth Promoting ◽  
Growth Promoting

Strain E19T described as Hartmannibacter diazotrophicus gen. nov. sp. nov. was isolated from the rhizosphere of Plantago winteri from a natural salt meadow in a nature protection area. Strain E19T is a plant growth-promoting rhizobacterium able to colonize the rhizosphere of barley and to promote its growth only under salt stress conditions. To gain insights into the genetic bases of plant growth promotion and its lifestyle at the rhizosphere under salty conditions, we determined the complete genome sequence using two complementary sequencing platforms (Ilumina MiSeq and PacBio RSII). The E19T genome comprises one circular chromosome and one plasmid containing several genes involved in salt adaptation and genes related to plant growth-promoting traits under salt stress. Based on previous experiments, ACC deaminase activity was identified as a main mechanism of E19T to promote plant growth under salt stress. Interestingly, no genes classically reported to encode for ACC deaminase activity are present. In general, the E19T genome provides information to confirm, discover, and better understand many of its previously evaluated traits involved in plant growth promotion under salt stress. Furthermore, the complete E19T genome sequence helps to define its previously reported unclear 16S rRNA gene-based phylogenetic affiliation. Hartmannibacter forms a distinct subcluster with genera Methylobrevis, Pleomorphomonas, Oharaeibacter, and Mongoliimonas subclustered with genera belonging to Rhizobiales.

Characterization of Root-Endophytic Actinobacteria From Cactus (Opuntia Ficus-Indica) for Plant Growth Promoting Traits

2021 ◽  
Author(s):  
V Govindasamy ◽  
Priya George ◽  
S V Ramesh ◽  
P Sureshkumar ◽  
Jagadish Rane ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Pgp Traits ◽  
Seedling Length ◽  
Plant Growth Promoting ◽  
Uninoculated Control ◽  
Growth Promoting ◽  
Endophytic Actinobacteria

Abstract The present study is the first report of isolation and characterization of endophytic actinobacteria from cactus (Opuntia ficus-indica). A total of 179 morphologically distinct endophytic actinobacterial isolates were purified from the roots of two different genetic accessions of cactus. All these isolates were screened for their plant growth promotion traits namely growth on N-free medium, P-solubilization, siderophore production, ACC deaminase activity and IAA production. A majority of the endophytic actinobacterial isolates (85%) exhibited the potential for plant growth promotion under in vitro conditions. Ten among the isolates were selected based on their multi-PGP traits and were identified as Streptomyces sp. based on the 16S rRNA gene sequencing and phylogenetic analysis. Plant growth promoting potential of these selected endophytic Streptomyces was studied in wheat seedlings. All these selected isolates significantly enhanced the growth parameters like seedling length and rootlets number compared to the uninoculated control. The wheat seeds inoculated with Streptomyces tuirus VL-70-IX exhibited maximum number of rootlets (6.33) compared to uninoculated control (3.67). The inoculation of endophytic actinobacteria Streptomyces pseudogriseolus VL-70-XII caused maximum seedling length (20.53 cm) and root length (8.26 cm) while the inoculation of S. radiopugnans HV-VIII resulted in highest shoot length (12.33 cm). These endophytic actinobacteria isolated from the roots of cactus accessions showed potential PGP traits. This work lays foundation for characterization and selection of endophytic actinobacteria from the under-exploited, drought tolerant species such as cactus with potential cross-compatibility for the improvement of plant growth of field crops especially under abiotic stress conditions..

scholarly journals The Root Microbiome of Salicornia ramosissima as a Seedbank for Plant-Growth Promoting Halotolerant Bacteria

2021 ◽  
Vol 11 (5) ◽  
pp. 2233
Author(s):  
Maria J. Ferreira ◽  
Angela Cunha ◽  
Sandro Figueiredo ◽  
Pedro Faustino ◽  
Carla Patinha ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Cultivated Plants ◽  
Plant Growth Promoting Bacteria ◽  
Crop Cultivation ◽  
Halotolerant Bacteria ◽  
Salicornia Ramosissima ◽  
Plant Growth Promoting ◽  
Growth Promoting

Root−associated microbial communities play important roles in the process of adaptation of plant hosts to environment stressors, and in this perspective, the microbiome of halophytes represents a valuable model for understanding the contribution of microorganisms to plant tolerance to salt. Although considered as the most promising halophyte candidate to crop cultivation, Salicornia ramosissima is one of the least-studied species in terms of microbiome composition and the effect of sediment properties on the diversity of plant-growth promoting bacteria associated with the roots. In this work, we aimed at isolating and characterizing halotolerant bacteria associated with the rhizosphere and root tissues of S. ramosissima, envisaging their application in saline agriculture. Endophytic and rhizosphere bacteria were isolated from wild and crop cultivated plants, growing in different estuarine conditions. Isolates were identified based on 16S rRNA sequences and screened for plant-growth promotion traits. The subsets of isolates from different sampling sites were very different in terms of composition but consistent in terms of the plant-growth promoting traits represented. Bacillus was the most represented genus and expressed the wider range of extracellular enzymatic activities. Halotolerant strains of Salinicola, Pseudomonas, Oceanobacillus, Halomonas, Providencia, Bacillus, Psychrobacter and Brevibacterium also exhibited several plant-growth promotion traits (e.g., 3-indole acetic acid (IAA), 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, siderophores, phosphate solubilization). Considering the taxonomic diversity and the plant-growth promotion potential of the isolates, the collection represents a valuable resource that can be used to optimize the crop cultivation of Salicornia under different environmental conditions and for the attenuation of salt stress in non-halophytes, considering the global threat of arable soil salinization.

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