scholarly journals Effects of Hair dyes on Sequence Analysis of Hair Mitochondrial DNA Hypervariable Region 1

2017 ◽  
Vol 11 (1) ◽  
pp. 61-66
Author(s):  
Halah Al-summarraie ◽  
Mohammed Al-Zubaidi ◽  
Dhuha Salim نعمة ◽  
Sura Nabeel Hameed ◽  
Thooalnoon Younes Saleh ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Sequence Analysis ◽  
Dna Extraction ◽  
Hypervariable Region ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Hair Dyes ◽  
Hypervariable Region 1 ◽  
Hair Samples ◽  
The Impact

Hair can be a valuable source of DNA especially in forensic casework and for noninvasive studies of human, when blood samples not available. This study emphasizes the impact of hair dyes on DNA sequence analysis. Samples collected from forty Iraqi families; each sample was divided in to two parts hair follicles and hair shaft. DNA extracted by using two different techniques, Phenol-chloroform (organic) method and prepFiler forensic DNA extraction kit. After quantification of DNA by real time PCR to confirm the exact DNA yield, Mitochondrial DNA (MtDNA) hypervariable region 1 successfully amplified from (50% of samples include hair follicle and 20% samples include shaft only), which all extracted  by organic method. Whereas by using prepFiler kit the ratio of amplification success reach to 95% of samples included hair follicles, but there wais no DNA outcome from hair shaft by using this kit. Our results demonstrate that treated hair by dyed or henna had a significant influence on the sequence analysis results. Organic method was an appropriate method for extraction DNA from hair shaft, since this method used for extracting the old and degraded samples. While prepFiler DNA extraction kit was more convenient for isolation DNA from, hair samples included follicles only with excellent result.

Nucleotide sequence analysis of the hypervariable region III of mitochondrial DNA in Thais

2016 ◽  
Vol 41 ◽  
pp. 10-14 ◽  
Author(s):  
Punlop Thongngam ◽  
Worraanong Leewattanapasuk ◽  
Tanin Bhoopat ◽  
Padchanee Sangthong
Keyword(s):  
Mitochondrial Dna ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Hypervariable Region ◽  
Nucleotide Sequence Analysis

scholarly journals Mitochondrial DNA hypervariable region 1 diversity in Nigerian goats – CORRIGENDUM

2017 ◽  
pp. 1
Author(s):  
Moses Okpeku ◽  
Sunday O. Peters ◽  
Ikhide G. Imumorin ◽  
Kyle C. Caires ◽  
Varun K. Sharma ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Hypervariable Region ◽  
Hypervariable Region 1

Mitochondrial DNA hypervariable region-1 sequence variation and phylogeny of the concolor gibbons,Nomascus

2007 ◽  
Vol 69 (11) ◽  
pp. 1285-1306 ◽  
Author(s):  
Keri Monda ◽  
Rachel E. Simmons ◽  
Philipp Kressirer ◽  
Bing Su ◽  
David S. Woodruff
Keyword(s):  
Mitochondrial Dna ◽  
Sequence Variation ◽  
Hypervariable Region ◽  
Hypervariable Region 1

scholarly journals ALKALINE LYSIS AS AN EFFICIENT AND ECONOMICAL ALTERNATIVE FOR DNA EXTRACTION FROM HORSEHAIR HAIR SAMPLES: COMPARISON BETWEEN DIFFERENT METHODS

2021 ◽  
Vol 9 (02) ◽  
pp. 836-841
Author(s):  
Myriam Janeth Ortega Torres ◽  
◽  
Jessica Almeida Braga ◽  
Camilo Torres ◽  
Ahmed Sami Shaker ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Hypervariable Region ◽  
Pcr Amplification ◽  
Extraction Methods ◽  
Forensic Population ◽  
Alkaline Lysis ◽  
Hair Samples ◽  
Genomic Studies ◽  
Dna Extraction Methods ◽  
Short Time

Studies related to DNA extraction are becoming more ambitious in the sense that large studies are intended to be carried out with minimum DNA sources. The DNA extracted must be of quality for genetic, forensic, population and genomic studies, these samples must be easy to obtain and product of efficient manipulation.Samples obtained from horsehair are an important technical challenge since they constitute the preferred non-invasive sample for genetic studies in horses, which has been shown to obtain reliable results in a short time. In this sense, working into effective techniques to optimizeDNA extraction of scarse samples is a pertinent task. In this study, different DNA extraction methods were evaluated from mane samples obtained from a population of wild horses from the Region of Arauca in eastern Colombia.Three DNA extraction methods were evaluated (phenol chloroform, alkaline lysis and twocommercial DNA extraction kit), DNA concentration, purity and qualityweredeterminate and PCR amplification product were obtain using primers for a hypervariable region of DNA mitochondrial. DNA preparation from hair roots using alkaline lysis was the most economical and efficient method with which it was possible to obtain high quality and quantity DNA.

scholarly journals Variability of mitochondrial DNA D-loop sequences in Zabaikalskaya horse breed

2021 ◽  
Vol 25 (5) ◽  
pp. 486-491
Author(s):  
L. A. Khrabrova ◽  
N. V. Blohina ◽  
B. Z. Bazaron ◽  
T. N. Khamiruev
Keyword(s):  
Mitochondrial Dna ◽  
Hypervariable Region ◽  
Composite Likelihood ◽  
Dna Polymorphisms ◽  
Nucleotide Position ◽  
Horse Breed ◽  
Bootstrap Analysis ◽  
Genetic Characteristics ◽  
Hair Samples ◽  
D Loop

The Zabaikalskaya horse is an indigenous breed of horses from Siberia with diverse use. It is characterized by e durance and good adaptability to year-round herd maintenance in the harsh conditions of the Baikal steppes. To determine the genetic characteristics of the maternal lineage of the Zabaikalskaya horse breed based on mitochondrial DNA polymorphisms, we collected hair samples from 31 horses belonging to breeding farms in the Trans-Baikal Territory. Analysis of the 530 bp sequence of the mtDNA D-loop was performed using the maximum composite likelihood (MCL) model in combination with bootstrap analysis. When studying the polymorphism of the hypervariable region of the mtDNA D-loop in Zabaikalskaya horses, we identified 31 haplotypes representing 8 haplogroups: B, C, G, H, L, M, Q and R according to modern classification. The sequenced fragment of the D-loop from nucleotide position 15471 to 16000 contained 17 polymorphic sites, mainly represented by the A→G, G→A and T→C transitions. The haplogroups Q (25.81 %), B (19.35 %), G (16.13 %) and H (12.90 %) were prevailing in the mtDNA structure of this breed. Genetic analysis of the mitochondrial genome of the Zabaikalskaya horse revealed a high level of diversity of haplotypes and haplogroups, which are typical for the horse populations of Eurasia.

scholarly journals Sequence analysis of mitochondrial DNA hypervariable region III of 400 Iraqi volunteers

2015 ◽  
Vol 14 (26) ◽  
pp. 2149-2156 ◽  
Author(s):  
Abdulhasan Kareem Muhanned ◽  
Amer Shneewer Hwazen ◽  
Ghazi Al Loza Nahla
Keyword(s):  
Mitochondrial Dna ◽  
Sequence Analysis ◽  
Hypervariable Region

Mitochondrial DNA hypervariable region 1 polymorphism in Singapore Chinese population

2005 ◽  
Vol 7 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Cheng-Yap Shee ◽  
Michelle S.M. Chong ◽  
Irene Ng ◽  
Tet-Fatt Chia
Keyword(s):  
Mitochondrial Dna ◽  
Chinese Population ◽  
Hypervariable Region ◽  
Hypervariable Region 1 ◽  
Singapore Chinese

Variation of the Hypervariable Region-1 of Mitochondrial DNA in Central-Eastern Italy

2003 ◽  
Vol 48 (2) ◽  
pp. 2002350 ◽  
Author(s):  
F. Verginelli ◽  
F. Donati ◽  
V. Coia ◽  
I. Boschi ◽  
R. Palmirotta ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Hypervariable Region ◽  
Hypervariable Region 1 ◽  
Central Eastern
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