Effect of Colloidal Gold Nanoparticles Preparation Conditions on Viability of MCF-7 Breast Cancer Cells

2020 ◽  
Vol 16 (4) ◽  
pp. 14-26
Author(s):  
Israa Hameed ◽  
Ammer Habeeb ◽  
Jasim Mansoor ◽  
Esam Hummadi
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Colloidal Gold ◽  
Breast Cancer Cells ◽  
Preparation Conditions ◽  
Colloidal Gold Nanoparticles ◽  
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The anticancer activity of chloroquine-gold nanoparticles against MCF-7 breast cancer cells

2012 ◽  
Vol 95 ◽  
pp. 195-200 ◽  
Author(s):  
Prachi Joshi ◽  
Soumyananda Chakraborti ◽  
Jaime E. Ramirez-Vick ◽  
Z.A. Ansari ◽  
Virendra Shanker ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Mcf 7

Gold nanoparticles induced apoptosis via oxidative stress and mitochondrial dysfunctions in MCF‐7 breast cancer cells

2020 ◽  
Vol 35 (1) ◽  
Author(s):  
Elumalai Kowsalya ◽  
Kithiyon MosaChristas ◽  
Chinna Rani Inbaraj Jaquline ◽  
Pannerselvam Balashanmugam ◽  
Thiyagarajan Devasena
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Mitochondrial Dysfunctions ◽  
Induced Apoptosis ◽  
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scholarly journals Radiosensitization of breast cancer cells using AS1411 aptamer-conjugated gold nanoparticles

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Somayeh Sadat Mehrnia ◽  
Bijan Hashemi ◽  
Seyed Javad Mowla ◽  
Maryam Nikkhah ◽  
Azim Arbabi
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Electron Beams ◽  
Clonogenic Assay ◽  
Cytotoxicity Studies ◽  
Radiation Induced ◽  
As1411 Aptamer ◽  
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Abstract Background Gold nanoparticles (GNPs) have been used to sensitize cancer cells and enhance the absorbed dose delivered to such cells. Active targeting can provide specific effect and higher uptake of the GNPs in the tumor cells, while having small effect on healthy cells. The aim of this study was to assess the possible radiosensitiazation effect of GNPs conjugated with AS1411 aptamer (AS1411/GNPs) on cancer cells treated with 4 MeV electron beams. Materials and methods Cytotoxicity studies of the GNPs and AS1411/GNPs were carried out with MTT and MTS assay in different cancer cell lines of MCF-7, MDA-MB-231 and mammospheres of MCF-7 cells. Atomic absorption spectroscopy confirmed the cellular uptake of the gold particles. Radiosensitizing effect of the GNPs and AS1411/GNPs on the cancer cells was assessed by clonogenic assay. Result AS1411 aptamer increased the Au uptake in MCF-7 and MDA-MB-231 cells. Clonogenic survival data revealed that AS1411/GNPs at 12.5 mg/L could result in radiosensitization of the breast cancer cells and lead to a sensitizer enhancement ratio of 1.35 and 1.66 and 1.91 for MCf-7, MDA-MB-231 and mammosphere cells. Conclusion Gold nanoparticles delivery to the cancer cells was enhanced by AS1411 aptamer and led to enhanced radiation induced cancer cells death. The combination of our clonogenic assay and Au cell uptake results suggested that AS1411 aptamer has enhanced the radiation-induced cell death by increasing Au uptake. This enhanced sensitization contributed to cancer stem cell-like cells to 4 MeV electron beams. This is particularly important for future preclinical testing to open a new insight for the treatment of cancers.

scholarly journals Cellular Uptake and Radio-sensitization Effect of Small Gold Nanoparticles in MCF-7 Breast Cancer Cells

2018 ◽  
Vol 09 (03) ◽  
Author(s):  
Branislava Janic ◽  
Fangchao Liu ◽  
Kevin R Bobbitt ◽  
Stephen L Brown ◽  
Indrin J Chetty ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Breast Cancer Cells ◽  
Sensitization Effect ◽  
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scholarly journals Gold Nanoparticles Increase PLK1-Specific Small Interfering RNA Transfection and Induce Apoptosis of Drug Resistance Breast Cancer Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Ying Tian ◽  
Yunlei Zhang ◽  
Jing Pan ◽  
Nan Lu ◽  
Shouju Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Drug Resistance ◽  
Cancer Cells ◽  
Small Interfering Rna ◽  
Breast Cancer Cells ◽  
Low Cytotoxicity ◽  
Interfering Rna ◽  
Lipofectamine 2000 ◽  
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Drug resistance is a major barrier that limits the effectiveness of chemotherapies against breast cancer. Here, gold nanoparticles (GNPs) characterized by good dispersivity, high stability, low cytotoxicity, and simple synthesis were developed to deliver small interfering RNA (siRNA) against PLK1 (PLK1-siRNA) and overcome the drug resistance of breast cancer cells. Compared with the commonly used Lipofectamine 2000, GNPs showed higher PLK1-siRNA delivery efficiency and resulted in the remarkable gene silencing ofPLK1in drug resistance breast cancer cells MCF-7/MDR1 with low cytotoxicityin vitro. Moreover, delivery of PLK1-siRNA by GNPs could cause 14.23% apoptosis of MCF-7/MDR1 cells, which was apparently higher than 11.01% apoptosis conducted by Lipofectamine 2000. In addition, GNPs showed strong X-ray attenuation coefficient, indicating the potential theranostic application of this system. Therefore, this study disclosed an important step in the use of GNPs as transfection vector of siRNA that will be of great benefit to gene therapy against drug resistant cancer.

Insights on the transport of tamoxifen by gold nanoparticles for MCF-7 breast cancer cells based on SERS spectroscopy

2018 ◽  
Vol 170 ◽  
pp. 712-717 ◽  
Author(s):  
Raïssa A.R. Teixeira ◽  
Alexandre A.B. Lataliza ◽  
Nádia R.B. Raposo ◽  
Luiz Antônio S. Costa ◽  
Antônio Carlos Sant’Ana
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Mcf 7
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