scholarly journals RB94, RB95, AI239 and AF209 antibodies recognize the Glutathione S-transferase protein by Western blot

2019 ◽  
Vol 2 (4) ◽  
pp. e61
Author(s):  
Otmane Lamrabet
Keyword(s):  
Western Blot ◽  
Recombinant Antibodies ◽  
Glutathione S Transferase

The recombinant antibodies RB94, RB95, AI239 and AF209 detect by Western blot the Glutathione S-transferase (GST) protein; AF212 does not.

scholarly journals Knockdown of Long Non-Coding RNA XIST Inhibited Doxorubicin Resistance in Colorectal Cancer by Upregulation of miR-124 and Downregulation of SGK1

2018 ◽  
Vol 51 (1) ◽  
pp. 113-128 ◽  
Author(s):  
Jia Zhu ◽  
Rui Zhang ◽  
Dongxiang Yang ◽  
Jibin Li ◽  
Xiaofei Yan ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Western Blot ◽  
Molecular Mechanisms ◽  
Luciferase Reporter ◽  
Regulatory Function ◽  
Glutathione S Transferase ◽  
Protein Levels ◽  
Qrt Pcr ◽  
Ic50 Value

Background/Aims: Doxorubicin (DOX) is a widely used chemotherapeutic agent for colorectal cancer (CRC). However, the acquirement of DOX resistance limits its clinical application for cancer therapy. Mounting evidence has suggested that aberrantly expressed lncRNAs contribute to drug resistance of various tumors. Our study aimed to explore the role and molecular mechanisms of lncRNA X-inactive specific transcript (XIST) in chemoresistance of CRC to DOX. Methods: The expressions of XIST, miR-124, serum and glucocorticoid-inducible kinase 1 (SGK1) mRNA in DOX-resistant CRC tissues and cells were detected by qRT-PCR or western blot analysis. DOX sensitivity was assessed by detecting IC50 value of DOX, the protein levels of P-glycoprotein (P-gp) and glutathione S-transferase-π (GST-π) and apoptosis. The interactions between XIST, miR-124 and SGK1 were confirmed by luciferase reporter assay, qRT-PCR and western blot. Xenograft tumor assay was used to verify the role of XIST in DOX resistance in CRC in vivo. Results: XIST expression was upregulated and miR-124 expression was downregulated in DOX-resistant CRC tissues and cells. Knockdown of XIST inhibited DOX resistance of CRC cells, as evidenced by the reduced IC50 value of DOX, decreased P-gp and GST-π levels and enhanced apoptosis in XIST-silenced DOX-resistant CRC cells. Additionally, XIST positively regulated SGK1 expression by interacting with miR-124 in DOX-resistant CRC cells. miR-124 suppression strikingly reversed XIST-knockdown-mediated repression on DOX resistance in DOX-resistant CRC cells. Moreover, SGK1-depletion-elicited decrease of DOX resistance was greatly restored by XIST overexpression or miR-124 inhibition in DOX-resistant CRC cells. Furthermore, XIST knockdown enhanced the anti-tumor effect of DOX in CRC in vivo. Conclusion: XIST exerted regulatory function in resistance of DOX possibly through miR-124/SGK1 axis, shedding new light on developing promising therapeutic strategy to overcome chemoresistance in CRC patients.

scholarly journals RA043 and RA044 antibodies recognize a peptide of the D. discoideum SibC protein by western blot

2020 ◽  
Vol 3 (3) ◽  
pp. e153
Author(s):  
Madeleine Zufferey ◽  
Cedric Blanc
Keyword(s):  
Western Blot ◽  
Dictyostelium Discoideum ◽  
Recombinant Antibodies

Recombinant antibodies RA043 and RA044 detect by western blot a peptide of the Dictyostelium discoideum SibC protein fused to a GST protein.

scholarly journals RB447, RB448, RB449, RB450, RB451 and RB453 antibodies recognize a Dictyostelium AlyL protein by Western Blot

2018 ◽  
Vol 1 (1) ◽  
pp. e2
Author(s):  
Otmane Lamrabet ◽  
Tania Jauslin
Keyword(s):  
Western Blot ◽  
Dictyostelium Discoideum ◽  
Recombinant Antibodies

The recombinant antibodies RB447, RB448, RB449, RB450, RB451 and RB453 detect by western blot the fulllength AlyL protein from Dictyostelium discoideum.

scholarly journals RB376 and RB377 antibodies recognize the Dictyostelium AlyA protein by Western blot

2019 ◽  
Vol 2 (1) ◽  
pp. e10
Author(s):  
Otmane Lamrabet
Keyword(s):  
Western Blot ◽  
Dictyostelium Discoideum ◽  
Recombinant Antibodies ◽  
Full Length

The recombinant antibodies RB376 and RB377 detect by Western blot the full-length AlyA protein from Dictyostelium discoideum. RB343, RB344 and RB378 antibodies do not.

scholarly journals AS739, AT693, AU197 and AU734 antibodies label the spike S protein from SARS-CoV-2 by western blot

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Célia Lazzarotto ◽  
Marie N. Schmid ◽  
Julien Ollivier ◽  
Alexandre P. Vaudano ◽  
Sara Da Fonte ◽  
...  
Keyword(s):  
Western Blot ◽  
Recombinant Antibodies ◽  
S Protein

The recombinant antibodies AS739, AT693, AU197 and AU734 detect by western blot the spike S protein from SARS-CoV-2.

scholarly journals The RA11 and RA12 antibodies recognize a peptide of the D. discoideum Mucolipin protein by western blot

2020 ◽  
Vol 3 (1) ◽  
pp. e127
Author(s):  
Madeleine Zufferey ◽  
Cedric Blanc
Keyword(s):  
Western Blot ◽  
Dictyostelium Discoideum ◽  
Recombinant Antibodies

The recombinant antibodies RA11 and RA12 detect by western blot a peptide of the Dictyostelium discoideum Mucolipin protein fused to a GST protein.

scholarly journals RB045 and RB048 antibodies recognize a peptide of the D. discoideum SibA protein by western blot

2020 ◽  
Vol 3 (3) ◽  
pp. e148
Author(s):  
Madeleine Zufferey ◽  
Cedric Blanc
Keyword(s):  
Western Blot ◽  
Dictyostelium Discoideum ◽  
Recombinant Antibodies

Recombinant antibodies RB045 and RB048 detect by western blot a peptide of the Dictyostelium discoideum SibA protein fused to a GST protein.

scholarly journals RB155, RB156 and RB189 antibodies do not recognize the D. discoideum Tsg101 protein by western blot

2019 ◽  
Vol 2 (4) ◽  
pp. e109
Author(s):  
Florence Leuba ◽  
Thierry Soldati
Keyword(s):  
Western Blot ◽  
Dictyostelium Discoideum ◽  
Recombinant Antibodies ◽  
Full Length

The recombinant antibodies RB155, RB156 and RB189 do not detect by western blot the full-length Tsg101 protein from Dictyostelium discoideum.

scholarly journals A Premature Termination of Human Epidermal Growth Factor Receptor Transcription inEscherichia coli

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Jihene Elloumi-Mseddi ◽  
Karim Jellali ◽  
Antonio Villalobo ◽  
Sami Aifa
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Western Blot ◽  
Blot Analysis ◽  
Growth Factor Receptor ◽  
Full Length ◽  
Glutathione S Transferase ◽  
Extracellular Region ◽  
Epidermal Growth

Our success in producing an active epidermal growth factor receptor (EGFR) tyrosine kinase inEscherichia coliencouraged us to express the full-length receptor in the same host. Despite its large size, we were successful at producing the full-length EGFR protein fused to glutathione S-transferase (GST) that was detected by Western blot analysis. Moreover, we obtained a majoritarian truncated GST-EGFR form detectable by gel electrophoresis and Western blot. This truncated protein was purified and confirmed by MALDI-TOF/TOF analysis to belong to the N-terminal extracellular region of the EGFR fused to GST. Northern blot analysis showed two transcripts suggesting the occurrence of a transcriptional arrest.

scholarly journals Thermoprotection of synaptic transmission in a Drosophila heat shock factor mutant is accompanied by increased expression of Hsp83 and DnaJ-1

2006 ◽  
Vol 25 (3) ◽  
pp. 493-501 ◽  
Author(s):  
Scott J. Neal ◽  
Shanker Karunanithi ◽  
Adrienne Best ◽  
Anthony Ken-Choy So ◽  
Robert M. Tanguay ◽  
...  
Keyword(s):  
Heat Shock ◽  
Western Blot ◽  
Dna Microarray ◽  
Blot Analysis ◽  
Heat Shock Factor ◽  
Temperature Sensitive ◽  
Glutathione S Transferase ◽  
Microarray Experiments ◽  
Drosophila Larvae ◽  
Shock Proteins

In Drosophila larvae, acquired synaptic thermotolerance after heat shock has previously been shown to correlate with the induction of heat shock proteins (Hsps) including HSP70. We tested the hypothesis that synaptic thermotolerance would be significantly diminished in a temperature-sensitive strain ( Drosophila heat shock factor mutant hsf4), which has been reported not to be able to produce inducible Hsps in response to heat shock. Contrary to our hypothesis, considerable thermoprotection was still observed at hsf4 larval synapses after heat shock. To investigate the cause of this thermoprotection, we conducted DNA microarray experiments to identify heat-induced transcript changes in these organisms. Transcripts of the hsp83, dnaJ-1 ( hsp40), and glutathione- S-transferase gstE1 genes were significantly upregulated in hsf4 larvae after heat shock. In addition, increases in the levels of Hsp83 and DnaJ-1 proteins but not in the inducible form of Hsp70 were detected by Western blot analysis. The mode of heat shock administration differentially affected the relative transcript and translational changes for these chaperones. These results indicate that the compensatory upregulation of constitutively expressed Hsps, in the absence of the synthesis of the major inducible Hsp, Hsp70, could still provide substantial thermoprotection to both synapses and the whole organism.

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