Late-phase reactions to intradermal testing with Dermatophagoides farinae in healthy dogs and dogs with house dust mite-induced atopic dermatitis

2002 ◽  
Vol 63 (1) ◽  
pp. 69-73 ◽  
Author(s):  
Andrew Hillier ◽  
Lynette K. Cole ◽  
Kenneth W. Kwochka ◽  
Catherine McCall
2012 ◽  
Vol 32 (9) ◽  
pp. 917-921 ◽  
Author(s):  
Victor E.S. Cunha ◽  
Maria Helena Silva ◽  
João Luiz H. Faccini

House dust mite antigens have been used for decades to diagnose allergic diseases in humans and animals. The objective of this study was to identify allergens in commercial Dermatophagoides farinae and Blomia tropicalis extracts by immunoblotting using sera from allergic dogs and anti-dog IgE conjugate. The analysis of antigens present in the D. farinae extract (FDA Allergenic) using sera from 10 dogs allergic to D. farinae showed that eight sera recognized a band of approximately 102 kDa, eight recognized two bands of 52 to 76 kDa, five recognized one band of approximately 76 kDa, four recognized one band of 31 to 38 kDa, and two recognized one band of 12 to 17 kDa. Immunoblot assays of the B. tropicalis extract (FDA Allergenic) using sera from 10 animals allergic to B. tropicalis showed that five sera recognized two bands of 52 to 76 kDa. These results demonstrate the importance of the two house dust mite species for the pathogenesis of canine atopic dermatitis in Brazil. In addition, the results indicate which allergens should be present in allergenic extracts used for diagnosis and allergen-specific immunotherapy.


Allergy ◽  
2001 ◽  
Vol 56 (12) ◽  
pp. 1226-1226 ◽  
Author(s):  
C. Gutgesell ◽  
S. Heise ◽  
S. Seubert ◽  
A. Seubert ◽  
S. Domhof ◽  
...  

2013 ◽  
Vol 132 (5) ◽  
pp. 1252-1254 ◽  
Author(s):  
Janneke Landheer ◽  
Barbara Giovannone ◽  
Jeanine D. Mattson ◽  
Sandra Tjabringa ◽  
Carla A.F.M. Bruijnzeel-Koomen ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document