scholarly journals Serological identification of house dust mite allergens in dogs with atopic dermatitis

2012 ◽  
Vol 32 (9) ◽  
pp. 917-921 ◽  
Author(s):  
Victor E.S. Cunha ◽  
Maria Helena Silva ◽  
João Luiz H. Faccini
Keyword(s):  
Atopic Dermatitis ◽  
House Dust ◽  
House Dust Mite ◽  
Allergic Diseases ◽  
Mite Species ◽  
Dermatophagoides Farinae ◽  
Allergen Specific Immunotherapy ◽  
Serological Identification ◽  
Allergenic Extracts ◽  
Dust Mite

House dust mite antigens have been used for decades to diagnose allergic diseases in humans and animals. The objective of this study was to identify allergens in commercial Dermatophagoides farinae and Blomia tropicalis extracts by immunoblotting using sera from allergic dogs and anti-dog IgE conjugate. The analysis of antigens present in the D. farinae extract (FDA Allergenic) using sera from 10 dogs allergic to D. farinae showed that eight sera recognized a band of approximately 102 kDa, eight recognized two bands of 52 to 76 kDa, five recognized one band of approximately 76 kDa, four recognized one band of 31 to 38 kDa, and two recognized one band of 12 to 17 kDa. Immunoblot assays of the B. tropicalis extract (FDA Allergenic) using sera from 10 animals allergic to B. tropicalis showed that five sera recognized two bands of 52 to 76 kDa. These results demonstrate the importance of the two house dust mite species for the pathogenesis of canine atopic dermatitis in Brazil. In addition, the results indicate which allergens should be present in allergenic extracts used for diagnosis and allergen-specific immunotherapy.

scholarly journals How reliable is anamnestic data in predicting the clinical relevance of house dust mite sensitization?

2021 ◽  
Author(s):  
Anna S. Englhard ◽  
Martin Holzer ◽  
Katharina Eder ◽  
Donata Gellrich ◽  
Moritz Gröger
Keyword(s):  
Atopic Dermatitis ◽  
House Dust ◽  
House Dust Mite ◽  
Clinical Relevance ◽  
Allergen Specific Immunotherapy ◽  
Provocation Tests ◽  
Nasal Provocation ◽  
Significant Difference ◽  
Dust Mite ◽  
Provocation Testing

Abstract Purpose For perennial inhalant allergens such as house dust mite (HDM), the German guideline on allergen-specific immunotherapy explicitly recommends provocation testing. This procedure is time-consuming, expensive, and potentially dangerous for the patient. Recently it has been discussed whether provocation tests are really necessary for diagnosing allergy to Dermatophagoides species. Thus, our study aimed to analyze the concordance between nasal provocation tests with Dermatophagoides species and anamnestic data. Methods We retrospectively analyzed the concordance between patients’ histories including self-reported symptom scores and the results of provocation testing in 471 individuals with proven sensitization to Dermatophagoides species. Results 248 patients had a positive nasal provocation test (NPT) result to Dermatophagoides species and 223 individuals a negative NPT result. Patients allergic to HSM suffered significantly more often from atopic dermatitis (14% vs. 7%, p = 0.046) and more from asthma (20% vs. 19%, p = 0.851). Moreover, individuals with clinically silent sensitization complained less about nasal secretion (37% vs. 45%, p = 0.244) but significantly more about nasal dryness (46% vs. 34%, p = 0.046) whereas rates of nasal airway obstruction, ocular complaints and sleep quality were comparable in both groups. Allergic patients reported more often perennial (34% vs. 30%, p = 0.374) and location-dependent (39% vs. 31%, p = 0.090) symptoms. However, the discrepant prevalence of atopic dermatitis was the only statistically significant difference between both groups. Conclusion Despite slight differences between both patient groups, clinical data are not sufficient to distinguish between silent sensitization and clinically relevant allergic rhinitis to HDM. Therefore, nasal provocation testing remains the gold standard for assessing clinical relevance in patients sensitized to Dermatophagoides species.

scholarly journals JENIS DAN KEPADATAN TUNGAU DEBU RUMAH PADA BEBERAPA HABITAT DI RUMAH PENDERITA PENYAKIT ALERGI

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Wiska F. Ponggalunggu ◽  
Victor D. Pijoh ◽  
Greta J. P. Wahongan
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Allergic Diseases ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Cross Sectional ◽  
Dust Mite ◽  
Euroglyphus Maynei

Abstract. House dust mites are the most found allergens in homes scattered throughout the world that associated with allergic manifestations in the respiratory tract and skin, such as bronchial asthma, allergic rhinitis and atopic dermatitis. The most common house dust mite is from the family Pyroglyphidae, four of which have a close relationship with the incidence of allergy: Dermatophagoides pteronyssinus, Dermatophagoides farinae, Dermatophagoides microceras, and Euroglyphus maynei. This study aims to determine the type and density of house dust mites in several habitats in the homes of people with allergic diseases. This study is a descriptive study using cross-sectional method. Samples were dust in allergic patient’s home in Malalayang I, taken from three habitats, namely bed (mattress and bed linen), bedrooms floor and sofa, as many as 96 samples. Of the 96 samples, there are 60 samples positive of dust mites and 36 negative samples. This study found 157 mites (124 adults and 33 larvae mites). Of the 124 adult mites were identified, there are 122 from order Astigmata with the dominant species is Dermatophagoides pteronyssinus, and two others of the order Prostigmata. In each habitat that is in the bed (mattress and bed linen), bedrooms floor and sofa, Pyroglyphidae is the most prevalent.Keywords: house dust mite, allergiesAbstrak. Tungau debu rumah (TDR) adalah alergen dalam rumah terbanyak yang tersebar di seluruh dunia yang dikaitkan dengan manifestasi alergi pada saluran pernapasan dan kulit, seperti asma bronkial, rinitis alergi dan dermatitis atopik. TDR yang paling sering ditemukan adalah dari famili Pyroglyphidae, empat di antaranya memiliki hubungan yang erat dengan kejadian alergi: Dermatophagoides pteronyssinus, Dermatophagoides farinae, Dermatophagoides microceras, dan Euroglyphus maynei. Penelitian ini bertujuan untuk mengetahui jenis dan kepadatan tungau debu rumah pada beberapa habitat di rumah penderita penyakit alergi. Penelitian ini adalah penelitian deskriptif dengan menggunakan metode cross-sectional. Sampel penelitian adalah debu di rumah penderita alergi di Kelurahan Malalayang I yang diambil dari 3 habitat, yaitu tempat tidur (kasur dan sprei), lantai kamar tidur, dan sofa sebanyak 96 sampel. Dari 96 sampel, 60 sampel positif tungau debu dan 36 sampel negatif. Pada penelitian ini ditemukan 157 tungau (124 tungau dewasa dan 33 larva). Dari 124 tungau dewasa yang terindentifikasi, dari ordo Astigmata sebanyak 122 dengan spesies yang dominan adalah Dermatophagoides pteronyssinus, dan dua lainnya dari ordo Prostigmata. Pada masing-masing habitat yaitu di tempat tidur (kasur dan sprei), lantai kamar tidur dan sofa, famili Pyroglyphidae paling banyak ditemukan.Kata kunci: tungau debu rumah, alergi

scholarly journals Identifikasi Tungau Debu Rumah di Tempat Tinggal Pasien Dermatitis Atopik RSUD Petala Bumi Pekanbaru

2019 ◽  
Vol 12 (2) ◽  
pp. 89
Author(s):  
Suri Dwi Lesmana ◽  
Deon Pradana Putra ◽  
Alida Widiawaty
Keyword(s):  
Atopic Dermatitis ◽  
House Dust ◽  
House Dust Mite ◽  
Sampling Technique ◽  
Allergic Diseases ◽  
House Dust Mites ◽  
Dust Mites ◽  
Hospital Design ◽  
Consecutive Sampling ◽  
Dust Mite

House dust mite have an important role as an allergens in allergic diseases such as atopic dermatitis. Atopic dermatitisis inflammation of the skin with itching, chronic and recurrent symptoms. The aim of this study was to find out thedescription of house dust mites in the house of patients at Petala Bumi Hospital. Design of study was a crosssectional.This study was occured from May-June 2018 in the Laboratory of Parasitology and involved 30 atopicdermatitis patients with consecutive sampling technique. Results is showed 100% of atopic dermatitis patients housewere positive for house dust mites, 63.3% of patients behaved badly in hygiene which affected degree of house dustmite spread. Conclusions of this study showed that a whole place of atopic dermatitis patients house was positive forhouse dust mite, most of patients behaved badly in hygiene which affected degree of house dust mite spread.

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