Effect of a syringe aspiration technique versus a mechanical suction technique and use of N-butylscopolammonium bromide on the quantity and quality of bronchoalveolar lavage fluid samples obtained from horses with the summer pasture endophenotype of equine asthma

Severe equine asthma (SEA) is a common, debilitating lower airway inflammatory disorder of older horses. Alveolar macrophages (AMs) survey inhaled particulates from barn sources causing them to switch from an anti-inflammatory to a proinflammatory phenotype, resulting in neutrophil recruitment to the lung. This proinflammatory switch may contribute to the development and prolongation of SEA. Validated antibodies to identify the cells involved in the pathogenesis of SEA are lacking. In this study, monoclonal antibodies against CD90, CD163, and CD206 were tested for reactivity with equine leukocytes by immunocytochemistry and flow cytometry. A multi-color flow cytometric assay was developed to identify leukocytes in equine bronchoalveolar lavage fluid (BALF). Four control and 4 SEA-susceptible horses had BALF collected before and after a 48-hour moldy hay challenge. Antibodies against CD90 uniquely labeled equine neutrophils, and antibodies against CD163 and CD206 identified equine macrophages. Postchallenge AM surface expression of CD163 increased in both groups of horses, but the increase was statistically significant in only the SEA-susceptible group ( P = .02). The surface expression of CD206 on AMs increased significantly in the SEA-susceptible group ( P = .03) but was unchanged in the control group ( P = .5). Increased expression of CD163 and CD206 during exacerbation of SEA suggested an association between AM phenotype and lung inflammation. However, functions of AMs in the pathogenesis of SEA remain to be elucidated.

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Tumor necrosis factor-alpha protein concentrations in bronchoalveolar lavage fluid from healthy horses and horses with severe equine asthma

Veterinary Immunology and Immunopathology ◽

10.1016/j.vetimm.2018.06.014 ◽

2018 ◽

Vol 202 ◽

pp. 70-73 ◽

Cited By ~ 1

Author(s):

Julia B. Montgomery ◽

Michelle L. Husulak ◽

Hayley Kosolofski ◽

Scott Dos Santos ◽

Hilary Burgess ◽

...

Keyword(s):

Tumor Necrosis Factor ◽

Bronchoalveolar Lavage ◽

Tumor Necrosis Factor Alpha ◽

Tumor Necrosis ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Necrosis Factor Alpha ◽

Factor Alpha ◽

Equine Asthma ◽

Necrosis Factor

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Respiratory metabolites in bronchoalveolar lavage fluid (BALF) and exhaled breath condensate (EBC) can differentiate horses affected by severe equine asthma from healthy horses

BMC Veterinary Research ◽

10.1186/s12917-020-02446-9 ◽

2020 ◽

Vol 16 (1) ◽

Author(s):

Marilena Bazzano ◽

Luca Laghi ◽

Chenglin Zhu ◽

Gian Enrico Magi ◽

Beniamino Tesei ◽

...

Keyword(s):

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Exhaled Breath Condensate ◽

Lavage Fluid ◽

Exhaled Breath ◽

Equine Asthma ◽

Breath Condensate

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Caution in Using Proteins Extracted from Exhaled Breath Condensate of Intubated Patients for Pediatric ARDS Studies

10.21203/rs.3.rs-64380/v1 ◽

2020 ◽

Author(s):

Mohit Ojha ◽

Rashika Joshi ◽

Brian Michael Varisco

Keyword(s):

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Exhaled Breath Condensate ◽

Invasive Mechanical Ventilation ◽

Lavage Fluid ◽

Exhaled Breath ◽

Silver Stain ◽

Discrete Band ◽

Breath Condensate

Abstract Objective We sought to analyze the quality of proteins extracted from the humidified moisture exchange filter (HMEF) of pediatric acute respiratory distress syndrome (PARDS) patients undergoing invasive mechanical ventilation.Results Proteins were extracted from ten filters from one control and six PARDS subjects. Between 0.69-1.69 mg of protein was extracted from each. Silver stain of these extracts identified only one discrete band compared to many discrete bands in mouse BALF. Liquid chromatography- mass spectrometry of this band and a corresponding band in mouse bronchoalveolar lavage fluid identified them as human and mouse albumin respectively. Multiple other non-degraded proteins were obvious in mouse bronchoalveolar lavage fluid but not proteins extracted from HMEFs.

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