Improved Agrobacterium-mediated genetic transformation of GNA transgenic sugarcane

Biologia ◽  
2007 ◽  
Vol 62 (4) ◽  
Author(s):  
Dongting Zhangsun ◽  
Sulan Luo ◽  
Rukai Chen ◽  
Kexuan Tang
Keyword(s):  
Resistance Gene ◽  
Medium Composition ◽  
Marker Genes ◽  
35S Promoter ◽  
Selective Marker ◽  
Sugarcane Cultivar ◽  
Transgenic Sugarcane ◽  
Woolly Aphid ◽  
Ceratovacuna Lanigera ◽  
Screenable Marker

AbstractSix plasmids carrying a snowdrop lectin (Galanthus nivalis agglutinin, GNA) and one of three selection markers were successfully transferred into two sugarcane cultivars (FN81–745 and Badila) via Agrobacterium-mediated transformation. Agrobacterium strains LBA4404, EHA105 and A281 that harboured a super-binary vector were used for sugarcane transformation. The use of the hygromycin (Hyg) resistance gene (hpt II), phosphinothrincin (PPT) resistance gene (bar) or G418 resistance gene (npt II) as a screenable marker facilitated the initial selection of GNA transgenic sugarcane callus with different efficiencies and helped the rapid segregation of individual transformation events. All the three selective marker genes were controlled by CaMV 35S promoter, while GNA gene was controlled by promoter of RSs-1 (rice sucrose synthase-1) or Ubi (maize ubiquitin). Factors important to successful transformation mediated by Agrobacterium tumefaciens were optimized, which included concentration of A. tumefaciens, medium composition, co-cultivated methods with plant tissue, strain virulence and different selective marker genes. An efficient protocol for sugarcane transformation mediated by A. tumefaciens was established. The GNA gene has been integrated into sugarcane genome as demonstrated by PCR and Southern dot blotting detections. The preliminary results from bioassay demonstrated a significant resistance of the transgenic sugarcane plants to woolly aphid (Ceratovacuna lanigera Zehnther) indicating thus the possibility for obtaining a transgenic sugarcane cultivar with resistance to woolly aphid.

Production and evaluation of transgenic sugarcane containing a Fiji disease virus (FDV) genome segment S9-derived synthetic resistance gene

2004 ◽  
Vol 55 (2) ◽  
pp. 139 ◽  
Author(s):  
R. B. McQualter ◽  
J. L. Dale ◽  
R. M. Harding ◽  
J. A. McMahon ◽  
G. R. Smith
Keyword(s):  
Resistance Gene ◽  
Disease Virus ◽  
Transgenic Line ◽  
Resistance Mechanism ◽  
Transcriptional Gene Silencing ◽  
Sugarcane Cultivar ◽  
Dna And Rna ◽  
Transgenic Sugarcane ◽  
Enhanced Resistance ◽  
Fiji Disease Virus

A transgenic line of the sugarcane cultivar Q124 with significantly enhanced resistance to Fiji disease was produced by microprojectile-mediated transformation with a transgene encoding a translatable version of Fiji disease virus (FDV) segment 9 ORF 1 under the control of the maize polyubiquitin promoter. Sixty-four transgenic lines were tested in glasshouse trials by caging the plants with viruliferous Perkinsiella saccharicida planthoppers. After 2 weeks, the planthoppers were removed and the plants monitored for symptoms. One transgenic line showed significantly enhanced resistance to Fiji disease compared with the Q124 parent and other lines showed varying levels of resistance. The molecular phenotypes of the transgenic plants at both the DNA and RNA levels were not entirely consistent with a resistance mechanism based on post-transcriptional gene silencing but were consistent with reports from other sugarcane-virus resistance systems. This is the first report of transgenic sugarcane containing an FDV-derived synthetic resistance gene showing resistance to FDV, although the mechanism of resistance has not yet been elucidated.

Genetic transformation of Nannochloropsis oculata with a bacterial phleomycin resistance gene as dominant selective marker

2016 ◽  
Vol 15 (2) ◽  
pp. 351-356 ◽  
Author(s):  
Xiaolei Ma ◽  
Kehou Pan ◽  
Lin Zhang ◽  
Baohua Zhu ◽  
Guanpin Yang ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Resistance Gene ◽  
Nannochloropsis Oculata ◽  
Selective Marker

scholarly journals Multiple repressor pathways contribute to phenotypic switching of vascular smooth muscle cells

2007 ◽  
Vol 292 (1) ◽  
pp. C59-C69 ◽  
Author(s):  
Keiko Kawai-Kowase ◽  
Gary K. Owens
Keyword(s):  
Smooth Muscle ◽  
Molecular Mechanisms ◽  
Platelet Derived Growth Factor ◽  
Phenotypic Switching ◽  
Marker Genes ◽  
Environmental Cues ◽  
Selective Marker ◽  
Current State ◽  
Active Repressor

Smooth muscle cell (SMC) differentiation is an essential component of vascular development and these cells perform biosynthetic, proliferative, and contractile roles in the vessel wall. SMCs are not terminally differentiated and possess the ability to modulate their phenotype in response to changing local environmental cues. The focus of this review is to provide an overview of the current state of knowledge of molecular mechanisms involved in controlling phenotypic switching of SMC with particular focus on examination of processes that contribute to the repression of SMC marker genes. We discuss the environmental cues which actively regulate SMC phenotypic switching, such as platelet-derived growth factor-BB, as well as several important regulatory mechanisms required for suppressing expression of SMC-specific/selective marker genes in vivo, including those dependent on conserved G/C-repressive elements, and/or highly conserved degenerate CArG elements found in the promoters of many of these marker genes. Finally, we present evidence indicating that SMC phenotypic switching involves multiple active repressor pathways, including Krüppel-like zinc finger type 4, HERP, and ERK-dependent phosphorylation of Elk-1 that act in a complementary fashion.

scholarly journals Radiation as a tool to remove selective marker genes from transgenic soybean plants

2006 ◽  
Vol 50 (1) ◽  
pp. 146-148 ◽  
Author(s):  
M. L. Tinoco ◽  
G. R. Vianna ◽  
S. Abud ◽  
P. I. M. Souza ◽  
E. L. Rech ◽  
...  
Keyword(s):  
Marker Genes ◽  
Transgenic Soybean ◽  
Selective Marker ◽  
Soybean Plants

A simple mass rearing method for dipha aphidivora, the pyralid predator of sugarcane woolly aphid ceratovacuna lanigera

Sugar Tech ◽  
2006 ◽  
Vol 8 (2-3) ◽  
pp. 160-165 ◽  
Author(s):  
N. Mukunthan ◽  
R. Nirmala ◽  
G. Santhalakshmi ◽  
J. Srikanth ◽  
B. Singaravelu
Keyword(s):  
Mass Rearing ◽  
Rearing Method ◽  
Woolly Aphid ◽  
Simple Mass ◽  
Ceratovacuna Lanigera
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