scholarly journals Experience with Imaging by Using of Microfocus X-Ray Source

2010 ◽  
Vol 61 (5) ◽  
pp. 287-290 ◽  
Author(s):  
Zdenko Zápražný ◽  
Dušan Korytár ◽  
František Dubecký ◽  
Vladimír Áč ◽  
Zbigniew Stachura ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Phase Contrast ◽  
Ccd Camera ◽  
X Ray ◽  
X Ray Imaging ◽  
Radiation Sources ◽  
Set Up ◽  
High Level ◽  
Very High

Experience with Imaging by Using of Microfocus X-Ray SourceIn this paper we present the current work and experience with using microfocus x-ray generator and commercial CCD camera for x-ray imaging purpose. There is a need in laboratories for the development of imaging methods approaching synchrotron radiation sources, where the brilliance of radiation is on very high-level. Generally, there is no continuous access to synchrotron facilities. Several synchrotron radiation laboratories allocate the access via a proposal system. Thus the time for synchrotron radiation experiments seldom exceeds more than 1-2 weeks per year, which restricts its application to a few selected experiments. Even in future, the routine characterization of samples will be performed mainly at the experimenters home laboratories [10]. In this contribution we show that with the present set-up it is possible to achieve the spatial resolution down to μm and with the appropriate geometry a phase contrast images are observable.

Characterization of mouse spinal cord vascular network by means of synchrotron radiation X-ray phase contrast tomography

2016 ◽  
Vol 32 (12) ◽  
pp. 1779-1784 ◽  
Author(s):  
Lorenzo Massimi ◽  
Michela Fratini ◽  
Inna Bukreeva ◽  
Francesco Brun ◽  
Alberto Mittone ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Synchrotron Radiation ◽  
Phase Contrast ◽  
Vascular Network ◽  
Mouse Spinal Cord ◽  
X Ray ◽  
Phase Contrast Tomography

Exploring experimental parameter choice for rapid speckle-tracking phase-contrast X-ray imaging with a paper analyzer

2015 ◽  
Vol 22 (5) ◽  
pp. 1279-1288 ◽  
Author(s):  
Isobel A. Aloisio ◽  
David M. Paganin ◽  
Christopher A. Wright ◽  
Kaye S. Morgan
Keyword(s):  
Real Time ◽  
Speckle Tracking ◽  
Phase Contrast ◽  
Experimental Parameter ◽  
Dynamic Imaging ◽  
Synchrotron Source ◽  
X Ray ◽  
Detector Distance ◽  
X Ray Imaging ◽  
Set Up

Phase-contrast X-ray imaging using a paper analyzer enables the visualization of X-ray transparent biological structures using the refractive properties of the sample. The technique measures the sample-induced distortions of a spatially random reference pattern to retrieve quantitative sample information. This phase-contrast method is promising for biomedical application due to both a simple experimental set-up and a capability for real-time imaging. The authors explore the experimental configuration required to achieve robustness and accuracy in terms of (i) the paper analyzer feature size, (ii) the sample-to-detector distance, and (iii) the exposure time. Results using a synchrotron source confirm that the technique achieves accurate phase retrieval with a range of paper analyzers and at exposures as short as 0.5 ms. These exposure times are sufficiently short relative to characteristic physiological timescales to enable real-time dynamic imaging of living samples. A theoretical guide to the choice of sample-to-detector distance is also derived. While the measurements are specific to the set-up, these guidelines, the example speckle images, the strategies for analysis in the presence of noise and the experimental considerations and discussion will be of value to those who wish to use the speckle-tracking paper analyzer technique.

CCD developments in x-ray imaging from synchrotron radiation sources

1997 ◽  
Author(s):  
Christian M. Castelli ◽  
John E. Ashton ◽  
Peter J. Pool
Keyword(s):  
Synchrotron Radiation ◽  
X Ray ◽  
X Ray Imaging ◽  
Radiation Sources

Performance characterization of hard x-ray imaging instruments at synchrotron radiation facility SPring-8

2006 ◽  
Author(s):  
Yasushi Ogasaka ◽  
Keisuke Tamura ◽  
Ryo Shibata ◽  
Akihiro Furuzawa ◽  
Tomokazu Nakamura ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Performance Characterization ◽  
X Ray ◽  
X Ray Imaging

Phase-Contrast X-Ray Imaging Using Both Vertically and Horizontally Expanded Synchrotron Radiation X-Rays with Asymmetric Bragg Reflection

1999 ◽  
Vol 38 (Part 2, No. 4B) ◽  
pp. L470-L472 ◽  
Author(s):  
Yasushi Kagoshima ◽  
Yoshiyuki Tsusaka ◽  
Kazushi Yokoyama ◽  
Kengo Takai ◽  
Shingo Takeda ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Phase Contrast ◽  
Bragg Reflection ◽  
X Rays ◽  
X Ray ◽  
X Ray Imaging

scholarly journals Visualization of protein crystals by high-energy phase-contrast X-ray imaging

2019 ◽  
Vol 75 (11) ◽  
pp. 947-958 ◽  
Author(s):  
Maxim Polikarpov ◽  
Gleb Bourenkov ◽  
Irina Snigireva ◽  
Anatoly Snigirev ◽  
Sophie Zimmermann ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Data Collection ◽  
Diffraction Data ◽  
Phase Contrast ◽  
High Energy ◽  
Protein Crystals ◽  
Cubic Phase ◽  
Macromolecular Crystallography ◽  
X Ray ◽  
X Ray Imaging

For the extraction of the best possible X-ray diffraction data from macromolecular crystals, accurate positioning of the crystals with respect to the X-ray beam is crucial. In addition, information about the shape and internal defects of crystals allows the optimization of data-collection strategies. Here, it is demonstrated that the X-ray beam available on the macromolecular crystallography beamline P14 at the high-brilliance synchrotron-radiation source PETRA III at DESY, Hamburg, Germany can be used for high-energy phase-contrast microtomography of protein crystals mounted in an optically opaque lipidic cubic phase matrix. Three-dimensional tomograms have been obtained at X-ray doses that are substantially smaller and on time scales that are substantially shorter than those used for diffraction-scanning approaches that display protein crystals at micrometre resolution. Adding a compound refractive lens as an objective to the imaging setup, two-dimensional imaging at sub-micrometre resolution has been achieved. All experiments were performed on a standard macromolecular crystallography beamline and are compatible with standard diffraction data-collection workflows and apparatus. Phase-contrast X-ray imaging of macromolecular crystals could find wide application at existing and upcoming low-emittance synchrotron-radiation sources.

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