scholarly journals Sifat Fisik dan Akseptabilitas Keju yang Ditambahkan CaCl2 Menggunakan Ekstrak Jahe Merah

2020 ◽  
Vol 22 (1) ◽  
pp. 29
Author(s):  
S. Y. V. Putri ◽  
W. S. Putranto ◽  
A. Pratama
Keyword(s):  
Milk Clotting ◽  
Fresh Cheese ◽  
Milk Clotting Activity

Penelitian ini bertujuan untuk mengetahui pengaruh penambahan berbagai konsentrasi kalsium klorida (CaCl2) pada proses koagulasi susu menggunakan ekstrak jahe merah terhadap sifat fisik dan akseptabilitas  keju. Penelitian menggunakan analisis statistik Rancangan Acak Lengkap (RAL) yang terdiri atas 3 tiga perlakuan penambahan CaCl2 dengan tingkat konsentrasi P1 (0,01% b/v), P2 (0,02% b/v) dan P3 (0,03% b/v) dengan pengulangan sebanyak 6  enam kali. Hasil penelitian menunjukan bahwa penambahan CaCl2 meningkatkan nilai persentase rendemen, dan milk clotting activity serta memberikan hasil yang sama terhadap akseptabilitas pada produk fresh cheese. Penambahan konsentrasi CaCl2 sebanyak 0,02% memberikan hasil terbaik dengan persentase rendemen sebesar 13,03% dan milk clotting activity sebesar 1165,39 SU/ml serta menunjukkan nilai akseptabilitas yang dapat diterima oleh panelis.

Quality Analysis of Fresh Cheese Prepared Using Wrightia tinctoria Proteases

2020 ◽  
Vol 16 (9) ◽  
pp. 1309-1317
Author(s):  
Anusha Rajagopalan ◽  
Bindhu O. Sukumaran
Keyword(s):  
Textural Properties ◽  
Artemia Salina ◽  
Quality Analysis ◽  
Human Consumption ◽  
Cheese Making ◽  
Phase Partitioning ◽  
Milk Clotting ◽  
Three Phase ◽  
Fresh Cheese ◽  
Wrightia Tinctoria

Background: Aqueous leaf extract of Wrightia tinctoria has been in use for artisanal cheese preparation in diverse parts of India. Scientific validation behind the milk clotting potential of W. tinctoria stem proteases purified through three-phase partitioning (TPP) has been attempted. However, its contribution to cheese quality standards has not been tried yet. Objective: To evaluate the suitability of three-phase partitioned W. tinctoria (WT) stem proteases in fresh cheese making. Methods: Fresh cheese was prepared using W. tinctoria TPP proteases and its characteristics were compared with that of commercial plant milk coagulant, Enzeco® (CEz) and rennin (CRn). Yield, organoleptic and textural properties, total fat, protein and moisture content of all cheese were determined according to FSSAI standards. Toxicity of the TPP proteases was assessed on the shrimp model (Artemia salina egg and Litopenaeus vannamei post-larval shrimps). Results: TPP proteases were found to be non-toxic and safe for human consumption with no change in egg hatchability and survival of the shrimps in comparison to that of control. Cheese analysis results indicated perceptible resemblance in nutritional characteristics of WT cheese with control cheese. Yield, textural properties and organoleptic acceptance of WT cheese resembled more closely with that of CEz cheese. Conclusion: Observation from the study paves the way for the acceptance of W. tinctoria proteases as a suitable vegetable rennet for fresh cheese making.

A novel milk-clotting enzyme from Aspergillus oryzae and A. luchuensis is an aspartic endopeptidase PepE presumed to be a vacuolar enzyme

2022 ◽  
Author(s):  
Yoko Takyu ◽  
Taro Asamura ◽  
Ayako Okamoto ◽  
Hiroshi Maeda ◽  
Michio Takeuchi ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Milk Clotting ◽  
Milk Clotting Enzyme ◽  
Proteolytic Activities ◽  
Homologous Enzyme ◽  
Traditional Fermentation ◽  
Enzyme Source ◽  
Cheese Production ◽  
Milk Clotting Activity ◽  
Aspartic Endopeptidase

Abstract Aspergillus oryzae RIB40 has 11 aspartic endopeptidase genes. We searched for milk-clotting enzymes based on the homology of the deduced amino acid sequence with chymosins. As a result, we identified a milk-clotting enzyme in A. oryzae. We expected other Aspergillus species to have a homologous enzyme with milk-clotting activity, and we found the most homologous aspartic endopeptidase from A. luchuensis had milk-clotting activity. Surprisingly, two enzymes were considered as vacuole enzymes according to a study on A. niger proteases. The two enzymes from A. oryzae and A. luchuensis cleaved a peptide between the 105Phe-106Met bond in κ-casein, similar to chymosin. Although both enzymes showed proteolytic activity using casein as a substrate, the optimum pH values for milk-clotting and proteolytic activities were different. Furthermore, the substrate specificities were highly restricted. Therefore, we expected that the Japanese traditional fermentation agent, koji, could be used as an enzyme source for cheese production.

Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases

LWT ◽  
2012 ◽  
Vol 47 (1) ◽  
pp. 103-109 ◽  
Author(s):  
Maria Alicia Corrons ◽  
Juan Ignacio Bertucci ◽  
Constanza Silvina Liggieri ◽  
Laura María Isabel López ◽  
Mariela Anahí Bruno
Keyword(s):  
Bioactive Peptides ◽  
Maclura Pomifera ◽  
Milk Clotting ◽  
Milk Clotting Activity
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