scholarly journals Analytical Electron Microscopic Studies of Calcium in Mouse Embryos during Blastocyst Formation

1982 ◽  
Vol 53 (4) ◽  
pp. 289-292
Author(s):  
Sueo NIIMURA ◽  
Kazuo ISHIDA
Author(s):  
Shou-kong Fan

Transmission and analytical electron microscopic studies of scale microstructures and microscopic marker experiments have been carried out in order to determine the transport mechanism in the oxidation of Ni-Al alloy. According to the classical theory, the oxidation of nickel takes place by transport of Ni cations across the scale forming new oxide at the scale/gas interface. Any markers deposited on the Ni surface are expected to remain at the scale/metal interface after oxidation. This investigation using TEM transverse section techniques and deposited microscopic markers shows a different result,which indicates that a considerable amount of oxygen was transported inward. This is the first time that such fine-scale markers have been coupled with high resolution characterization instruments such as TEM/STEM to provide detailed information about evolution of oxide scale microstructure.


1995 ◽  
Vol 74 (11) ◽  
pp. 1756-1763 ◽  
Author(s):  
Y. Miake ◽  
T. Yanagisawa ◽  
Y. Yajima ◽  
H. Noma ◽  
N. Yasui ◽  
...  

1978 ◽  
Vol 9 (8) ◽  
Author(s):  
M. J. TRICKER ◽  
D. A. JEFFERSON ◽  
J. M. THOMAS ◽  
P. G. MANNING ◽  
C. J. ELLIOTT

Author(s):  
Michael J. Tricker ◽  
David A. Jefferson ◽  
John M. Thomas ◽  
Philip G. Manning ◽  
Christopher J. Elliott

1988 ◽  
Vol 71 (9) ◽  
pp. 713-719 ◽  
Author(s):  
CHIN-JONG CHAN ◽  
WALTRAUD M. KRIVEN ◽  
J. FRANCIS YOUNG

Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.


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