Development and Validation of UV Spectroscopic Method for Simultaneous Estimation of Pantoprazole and Cinitapride in Bulk and in Capsule Dosage FormC

2018 ◽  
Vol 9 (4) ◽  
Author(s):  
Gaur A ◽  
Yashwant .
Keyword(s):  
Dosage Form ◽  
Spectroscopic Method ◽  
Pharmaceutical Formulations ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Routine Analysis ◽  
Simultaneous Estimation ◽  
Development And Validation

A new, rapid, precise, selective and sensitive Vierodt���s/simultaneous equation method is developed for the simultaneous estimation of pantoprazole (PNT) and cinitapride (CNT) in combined dosage form. In the developed method, absorbance was measured at 289 nm (�� max of Pantoprazole) and 267.2 nm (�� max of Cinitapride). The drugs obeyed the Beer���s law in the concentration range of 13-65��g/ml and 1-5 ��g/ml respectively for pantoprazole and cinitapride. Accuracy of the method was determined by recovery studies and was found to be 101.32 % and 98.9 % for Pantoprazole and Cinitapride respectively. The developed method is simple, precise, rapid and selective. It can be used for routine analysis of both drugs in bulk as well as in pharmaceutical formulations.

DEVELOPMENT AND VALIDATION OF A SENSITIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF PITAVASTATIN CALCIUM AND FENOFIBRATE

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (08) ◽  
pp. 17-21
Author(s):  
A Kukrety ◽  
◽  
K. Kohli ◽  
M. Singhal ◽  
C Dhal ◽  
...  
Keyword(s):  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Equation ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Standard Additions ◽  
Pitavastatin Calcium ◽  
Development And Validation ◽  
Different Levels ◽  
Sensitive Spectrophotometric Method

A simple, rapid, accurate, precise and reproducible UV spectroscopic method has been developed for the simultaneous estimation of pitavastatin calcium and fenofibrate in bulk and pharmaceutical dosage form. The method is based on simultaneous equation method. Pitavastatin calcium and fenofibrate have absorption maxima (λmax) at 245 nm and 286 nm respectively. Beer’s law was obeyed in the concentration range of 2-12 μg/mL and 32-192 μg/mL for pitavastatin calcium and fenofibrate, respectively. The recovery studies are indicative of accuracy of method and are found in between 97.66-107.22% and 103.33-110.55% for pitavastatin calcium and fenofibrate, respectively, at three different levels of standard additions. Precision studies showed satisfactory results.

scholarly journals Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Valsartan and Hydrochlorothiazide in Tablet Dosage Form

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Monika L. Jadhav ◽  
Manoj V. Girase ◽  
Shripad K. Tidme ◽  
Manish S. Junagade
Keyword(s):  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Simultaneous Equation ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Spectrophotometric Methods ◽  
Absorbance Ratio ◽  
Standard Additions ◽  
Development And Validation ◽  
Tablet Dosage

Two UV-spectrophotometric methods have been developed and validated for simultaneous estimation of valsartan and hydrochlorothiazide in a tablet dosage form. The first method employed solving of simultaneous equations based on the measurement of absorbance at two wavelengths, 249.4 nm and 272.6 nm, λmax for valsartan and hydrochlorothiazide, respectively. The second method was absorbance ratio method, which involves formation of Q-absorbance equation at 258.4 nm (isoabsorptive point) and also at 272.6 nm (λmax of hydrochlorothiazide). The methods were found to be linear between the range of 5–30 µg/mL for valsartan and 4–24 μg/mL for hydrochlorothiazide using 0.1 N NaOH as solvent. The mean percentage recovery was found to be 100.20% and 100.19% for the simultaneous equation method and 98.56% and 97.96% for the absorbance ratio method, for valsartan and hydrochlorothiazide, respectively, at three different levels of standard additions. The precision (intraday, interday) of methods was found within limits (RSD<2%). It could be concluded from the results obtained in the present investigation that the two methods for simultaneous estimation of valsartan and hydrochlorothiazide in tablet dosage form are simple, rapid, accurate, precise and economical and can be used, successfully, in the quality control of pharmaceutical formulations and other routine laboratory analysis.

scholarly journals Development and Validation of UV Spectrophotometric Method for the Estimation of Cilnidipine and Valsartan in Bulk and Pharmaceutical Formulations

2019 ◽  
Vol 12 (4) ◽  
pp. 35-42
Author(s):  
Deshmukh Madhuri D ◽  
Deshmukh Supriya D
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Low Cost ◽  
Pharmaceutical Formulations ◽  
Simultaneous Equation ◽  
Simultaneous Estimation ◽  
Coefficient Of Correlation ◽  
Development And Validation ◽  
Tablet Dosage ◽  
Complex Extraction

A simple, rapid, accurate, precise, specific and economical spectrophotometric method for simultaneous estimation of Cilnidipine and Valsartan in combined tablet dosage form has been developed. Its employs Formation and solving of simultaneous equation using two wavelengths 240.20nm and 250nm using methanol as solvent. This method obeys Beers law in the employed concentration range 2-12μg /mL and 8-40 μg /mL for Cilnidipine and Valsartan respectively. Coefficient of correlation (R2) was 0.999for Cilnidipine and 0.999 for Valsartan. This method can be adopted in routine analysis of Cilnidipine and Valsartan in bulk and tablet dosage form and it involves relatively low cost solvent and no complex extraction technique.

scholarly journals DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF VILANTEROL AND FLUTICASONE FUROATE IN PHARMACEUTICAL FORMULATIONS

2017 ◽  
Vol 10 (4) ◽  
pp. 302
Author(s):  
Siva Kishore Masimukku ◽  
Rambabu Chintal
Keyword(s):  
Method Development ◽  
Limit Of Detection ◽  
Pharmaceutical Formulations ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Fluticasone Furoate ◽  
Limit Of Quantification ◽  
Spectrophotometric Methods ◽  
Development And Validation

Objective:  To develop a simple, rapid,  precise, accurate, sensitive spectrophotometric methods (A&B) were developed for simultaneous estimation and validation of Vilanterol (VTL) and Fluticasone Furoate (FFE) in pure and tablet dosage forms.Method:   Method A is a simultaneous equation method and method B is a first order derivative spectrophotometric method. Pure drug samples of VTL and FFE were dissolved in a mixture of Methanol and Ethanol in the ratio of 1:1 (v/v) and found to have absorbance maxima at 231nm for VTL and 260nm for FFE respectivelyResults:  The linearity lies between 2.5–10µg/ml for VTL and 10–60µg/ml for FFE in these two methods (A&B).  The correlation coefficient (r2) was found to be 0.999 for both VTL and FFE, the limit of detection and limit of quantification were found to be 0.015µg/ml and 0.05µg/ml for VTL and 0.05µg/ml and 0.2µg/ml for FFE respectively. The results of analysis have been validated statistically by recovery studies as per ICH guidelines.Conclusion: The two methods A&B showed good reproducibility and recovery with % RSD less than 2.  Hence both methods were found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of VTL and FFE in pure and combined dosage form.Keywords: Fluticasone furoate, Vilanterol, Derivative spectrophotometric, Simultaneous equation method, Method development and validation.

scholarly journals Quantification of Benazepril Hydrochloride and Hydrochlorothiazide in Tablet Dosage Form by Simultaneous Equation Spectrophotometric Method

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Varsha Parmar ◽  
Usmangani Chhalotiya ◽  
Dimal Shah ◽  
Kashyap Bhatt ◽  
Sunil Baldania
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Routine Analysis ◽  
Simultaneous Estimation ◽  
Tablet Dosage

Simple, accurate, precise, reproducible, requiring no prior separation, and economical procedures for simultaneous estimation of benazepril hydrochloride (BEN) and Hydrochlorothiazide (HCT) in tablet dosage form have been developed. Simultaneous equation method employs for estimation of both drugs in methanol at 240 nm and 270 nm as two analytical wavelengths. BEN and HCT at their respective λmax (240 nm and 270 nm) show linearity in a concentration range of 2–12 μg/mL and 4–14 μg/mL. Recovery studies for BEN are 100.0–100.6% and 99.8–100.0% for HCT in the case of simultaneous equation method confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since it is rapid, simple, accurate and also sensitive and specific.

DEVELOPMENT OF STABILATING INDICATING ANALYTICAL METHOD FOR FAMOTIDINE AND DICLOFENAC POTASSIUM IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (06) ◽  
pp. 40-42
Author(s):  
D. R. Chaple ◽  
◽  
S. A Mehta ◽  
M. P Yeole ◽  
P. S. Tarte
Keyword(s):  
Analytical Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Routine Analysis ◽  
Simultaneous Estimation ◽  
Tablet Dosage

This work deals with the simultaneous estimation of famotidine and diclofenac potassium in a tablet dosage form. The method employed is simultaneous equation. The absorption maxima at 266 nm and 286 nm were used for the estimation of famotidine and diclofenac, respectively. Both the drugs and their mixture obey Beer-Lamberts law at selected wavelength in given concentration range. The result of analysis has been validated statistically and recovery studies confirmed the accuracy of the proposed method. The proposed method is simple, rapid, precise and accurate. It is used for the routine analysis of both drugs.

NEW DEVELOPED AND VALIDATED SPECTROSCOPIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TERBINAFINE HYDROCHLORIDE AND FLUCONAZOLE

2020 ◽  
pp. 19-25
Author(s):  
BHUMIKA THAKUR ◽  
INDER KUMAR
Keyword(s):  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Equation ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Pharmaceutical Dosage Form ◽  
Terbinafine Hydrochloride ◽  
Precision Study ◽  
Reproducible Method ◽  
Concentration Levels

Objective: A new, simple, precise, accurate, and reproducible method or simultaneous equation method was developed and validated for the simultaneous estimation of terbinafine hydrochloride (TH) and fluconazole (FLZ) in pure form. Methods: Simultaneous estimation of terbinafine hydrochloride and fluconazole was estimated by the ultraviolet (UV) spectrophotometry method. The method was based on the measurement of absorbance at two wavelengths 222 nm and 239 nm, of terbinafine hydrochloride and fluconazole in 0.1N HCl respectively. Results: Calibration curves terbinafine hydrochloride and fluconazole were found to be linear in the concentration ranges of 0.5-3.0 μg/ml and 80-400 μg/ml, respectively, with their correlation coefficient values (R2) 0.999 and 0.998. LOD and LOQ of TH were found to be 0.067, 0.203 at 222 nm and 0.175, 0.531 at 239 nm, similarly for FLZ; 31.089, 94.210 at 222 nm and 94.380, 286.00 at 239 nm respectively. In the precision study, the % RSD value was found within limits (%). The percentage recovery at various concentration levels varied from 98.50 % to 103.96 % for TH and 97.27 % to 103.83 % for FLZ confirming that the expected method is accurate. Conclusion: It could be concluded from the results obtained in the present study that this method for simultaneous estimation of TH and FLZ in pure is simple, precise, and economical. The proposed method can be applied successfully for the simultaneous estimation of TH and FLZ in the pure and pharmaceutical dosage form.

scholarly journals Development and validation of analytical method for Irbesartan and Atorvastatin by simultaneous equation spectroscopic method

2015 ◽  
Vol 1 (4) ◽  
pp. 194
Author(s):  
Paras B Virani ◽  
Rajanit Sojitra ◽  
Hasumati Raj ◽  
Vineet Jain
Keyword(s):  
Spectroscopic Method ◽  
Simultaneous Equation ◽  
Simultaneous Estimation ◽  
Limit Of Quantification ◽  
Limit Of Determination ◽  
Relative Standard ◽  
Acceptance Range ◽  
High Concentrations ◽  
Development And Validation ◽  
Validation Of Analytical Method

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Irbesartan and atorvastatin in synthetic mixture using simultaneous equation Method. In this spectroscopic method, 226.00 nm and 246.00 nm wavelengths were selected for measurement of absorptivity. Both the drugs show linearity in a concentration range of 05-30 ?g/ml at their respective ?max. Accuracy, precision and recovery studies were done by QC samples covering lower, medium and high concentrations of the linearity range. The relative standard deviation for accuracy, precision studies were found to be within the acceptance range (<2%). The limit of determination was 0.033?g/ml and 0.125 ?g/ml for Irbesartan and atorvastatin, respectively. The limit of quantification was 0.1008 ?g/ml and 0.3792 ?g/ml for Irbesartan and atorvastatin, respectively. Recovery of Irbesartan and atorvastatin were found to be 99.75 % and 99.52% respectively confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since they are rapid, simple, accurate and also sensitive and specific by no heating and no organic solvent extraction.

scholarly journals Simultaneous Spectrophotometric Method for Determination of Emtricitabine and Tenofovir Disoproxil Fumarate in Three-Component Tablet Formulation Containing Rilpivirine Hydrochloride

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
S. Venkatesan ◽  
N. Kannappan
Keyword(s):  
Spectrophotometric Method ◽  
Analytical Method ◽  
Tenofovir Disoproxil Fumarate ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Tablet Dosage

Developing a single analytical method for estimation of individual drug from a multidrug composition is a very challenging task. A complexation, derivatization, extraction, evaporation, and sensitive-free direct UV spectrophotometric method is developed and validated for the simultaneous estimation of some antiviral drugs such as emtricitabine (EMT), tenofovir disoproxil fumarate (TDF), and rilpivirine HCl (RPV) in tablet dosage form by Vierordt’s method. The solutions of standard and sample were prepared in methanol. The λmax⁡ for emtricitabine, tenofovir disoproxil fumarate, and rilpivirine hydrochloride were 240.8 nm, 257.6 nm, and 305.6 nm, respectively. Calibration curves are linear in the concentration ranges 4–12 μg/ml for EMT, 6–18 μg/ml for TDF, and 0.5–1.5 μg/ml for RPV, respectively. Results of analysis of simultaneous equation method were analyzed and validated for various parameters according to ICH guidelines.

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