Detection and assesment of red clover genetic sources with self-compatibility for development the varieties with high and sustainable seed productivity

Author(s):  
М.Ю. Новосёлов ◽  
О.А. Старшинова ◽  
Л.В. Дробышева ◽  
Г.П. Зятчина

Основными лимитирующими факторами в развитии клеверосеяния являются дефицит семян и их высокая стоимость. Наши исследования были направлены на поиск, выделение и изучение самосовместимых генотипов клевера лугового (Trifolium pratense L.). Потомство образца № 3000 I1, генетического источника самосовместимости, обладало данным свойством на уровне от 18 до 100 %. Средняя величина завязываемости семян при самоопылении составила 77 %, что сопоставимо с результатом при перекрёстном опылении (93 %) и многократно превосходит показатель самосовместимости у диплоидных сортов (0,5–1 %). Все изученные формы обладали завязываемостью семян на уровне 46 % без триппинга цветков при механическом воздействии на соцветия. Цитологическое изучение пыльцы и анализирующие скрещивания с диплоидными формами показали, что данный образец имеет диплоидное состояние. Сравнение по основным морфо-биологическим признакам образца № 3000 I1 с сортами Ранний 2 и ВИК 7 показало, что он отличается более продолжительным вегетационным периодом, низкорослостью, формирует большее количество стеблей, отличается повышенной облиственностью, содержанием сухого вещества и протеина. Выявлена высокая наследуемость самосовместимости в реципрокных скрещиваниях с сортом Ранний 2. Уровень самосовместимости более 50 % в поколении F1 был достигнут у 53 % генотипов при прямых и 74 % — при обратных скрещиваниях. Таким образом, созданный исходный материал может использоваться в селекционной практике для получения гомозиготного линейного материала и создания многолинейных сортов клевера лугового с высокой и устойчивой семенной продуктивностью. The basic limiting factor in development of clover-sowing is the deficit and high cost of seeds. Our investigations were directed on search, selection and study of self-compatible genotypes of red clover (Trifolium pratense L.). Progeny of a genetic source with self-compatibility (№ 3000 I1) exhibited this property with the level from 18 to 100 %. The average value of seed setting was 77 % at self-pollination, which was comparable with this parameter at cross-pollination — 93 %, and exceeded in many times self-compatibility of diploid varieties — 0.5–1 %. Every investigated genotype set seeds by 46 % in the absence of tripping, by mechanical stimulation of inflorescence. Cytology study of pollen and analyzing crossings with diploid forms have shown that studied sample is diploid. The № 3000 I1 and the varieties “Ranniy 2” and “VIK 7” were compared on morphological characters and biological properties. The studied sample had longer vegetative period, abundant foliage, was stunting, formed more stems, and contained more dry matter and protein. Self-compatibility highly inherited at reciprocal crossing with diploid variety “Ranniy 2”. Among progeny F1, at the direct crossings we have selected 53 % of genotypes with 50 % rate of self-compatibility and at the backcrossing — 74 %. Thus, the studied initial material is recommended to use in red clover breeding for creation homozygous lines and multilinear varieties with high and sustainable seed productivity.  

Euphytica ◽  
1973 ◽  
Vol 22 (3) ◽  
pp. 535-542 ◽  
Author(s):  
J. F. Duncan ◽  
M. K. Anderson ◽  
N. L. Taylor

2016 ◽  
Vol 15 (4) ◽  
pp. 376-379 ◽  
Author(s):  
Mamta Gupta ◽  
Vikas Sharma ◽  
Sunil K. Singh ◽  
Rakesh K. Chahota ◽  
Tilak R. Sharma

AbstractGenetic diversity of a red clover global collection was assessed using 36 simple sequence repeat (SSR) primers representing all seven linkage groups (LGs). The number of fragments amplified ranged from 1 to 6 for all the primers. Primer RCS0060 detected highest number of fragments, whereas four SSRs viz., RCS0899, RCS1594, TPSSR40 and RCS6927 amplified single fragment. Size range of amplicons generated by all the primers varied from 100 to 400 bp. Polymorphism information content values ranged from 0.301 to 0.719 with an average value of 0.605. LG wise diversity analysis showed that LG 3 was most diverse (I = 0.65, Ht = 0.44), whereas LG-1 showed minimum diversity (I = 0.48, Ht = 0.26) for the microsatellites used. Bayesian model-based clustering inferred three genetically distinct populations in the red clover germplasm holding and showed considerable admixture in individuals within clusters. Neighbour-joining analysis showed intermixing of accessions within groups. Principal component analysis plot complemented the clustering shown by Structure and distinguished three populations to greater extent. Analysis of molecular variance showed that 91% of the genetic variation was residing within populations, while 9% variation was among populations. Overall, the results showed that a high level of genetic diversity is prevailing in this worldwide collection of red clover, which can be exploited for its genetic improvement through breeding approaches.


Genetika ◽  
2014 ◽  
Vol 46 (3) ◽  
pp. 949-961 ◽  
Author(s):  
Ramadan Ahsyee ◽  
S Sanjavasiljevic ◽  
Irena Calic ◽  
Miroslav Zoric ◽  
Djura Karagic ◽  
...  

Red clover (Trifolium pratense L.) is one of the main forage species from temperate regions and it's originated in Eurasia where wild-type populations are found in the Caucasus Mountains. Considerable variation for this crop has been recorded for few molecular variations. The identification and understanding of molecular genetic diversity in red clover accessions will help in effective genetic conservation along with efficient breeding programs in this crop. This study has shown molecular diversity using microsatellite markers in red clover accessions from around of the world. In this investigation, 40 genotypes have been selected and analyzed using 15 SSR primers. These primer pairs amplified 1146 polymorphic loci among the genotypes screened. The number of fragments amplified by each SSR primer combination varied from 24 for RCS3681 to 109 for RCS1729 with an average value of 80.78 per primer combination. The SSR marker data was further analyzed using cluster algorithms and Principal coordinates analysis (PCoA). The results indicated that the considerable genetic variations were discovered among the analyzed genotypes. The SSR based clustering could identify the putative pedigree types of the present red clover types of diverse origins.


Crop Science ◽  
1965 ◽  
Vol 5 (5) ◽  
pp. 425-428 ◽  
Author(s):  
R. J. Bula ◽  
R. G. May ◽  
C. S. Garrison ◽  
C. M. Rincker ◽  
J. G. Dean

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