Detection of Cryptosporidium spp., Cyclospora spp. and Cystoisospora spp. cysts in faecal samples of immunocompromised patients using real-time PCR

2016 ◽  
Author(s):  
Varol Tunali
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Immunocompromised Patients ◽  
Cryptosporidium Spp ◽  
Faecal Samples

scholarly journals Development and validation of a triplex real-time PCR for rapid detection and specific identification of M. avium sub sp. paratuberculosis in faecal samples

2009 ◽  
Vol 136 (1-2) ◽  
pp. 166-172 ◽  
Author(s):  
Léonid M. Irenge ◽  
Karl Walravens ◽  
Marc Govaerts ◽  
Jacques Godfroid ◽  
Valérie Rosseels ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Specific Identification ◽  
Faecal Samples ◽  
Development And Validation

scholarly journals False Negative Results in High Viremia Parvovirus B19-Samples Tested with Real-Time PCR

2010 ◽  
Vol 59 (2) ◽  
pp. 129-132 ◽  
Author(s):  
PIOTR GRABARCZYK ◽  
ALEKSANDRA KALIŃSKA ◽  
EWA SULKOWSKA ◽  
EWA BROJER
Keyword(s):  
Positive Result ◽  
Real Time ◽  
Real Time Pcr ◽  
Parvovirus B19 ◽  
False Negative ◽  
Immunocompromised Patients ◽  
Negative Results ◽  
False Negative Results ◽  
Parvovirus B 19 ◽  
The Way

Extremely high viremia is observed during some viruses infection, especialy in immunocompromised patients. False negative results of Parvovirus B 19 DNA tests performed with real-time PCR in high viremic samples are reported. The way of fluorescence diagrams analysis and algorithm of positive result confirmation to exclude such phenomenon are proposed.

scholarly journals Cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor

2021 ◽  
Vol 41 (5) ◽  
pp. 293-298
Author(s):  
Mehmet Karabey ◽  
Hüseyin Can ◽  
Tülay Öncü Öner ◽  
Mert Döşkaya ◽  
Sedef Erkunt Alak ◽  
...  
Keyword(s):  
Sample Size ◽  
Real Time ◽  
Solid Tumors ◽  
Real Time Pcr ◽  
Tertiary Care ◽  
Diagnostic Methods ◽  
Cross Sectional ◽  
Cryptosporidium Spp ◽  
Stool Samples ◽  
Pcr Targeting

BACKGROUND: Cryptosporidium spp . is a protozoan parasite that infects many vertebrate animals, including humans. Since Cryptosporidium spp . can cause chronic life-threatening diarrhea and severe malabsorption in immunocompromised patients, we investigated the prevalence of this parasite among patients undergoing chemotherapy for malignant solid tumors. OBJECTIVE: Investigate the prevalence of Cryptosporidium spp . in stool samples. DESIGN: Cross-sectional. SETTING: Tertiary care. PATIENTS AND METHODS: Stool samples were collected from adult patients with malignant solid tumors receiving chemotherapy and diarrhea. Cryptosporidium spp . prevalence was determined using Ziehl–Neelsen staining, ELISA, and real-time PCR targeting of the COWP gene. MAIN OUTCOME MEASURE: The prevalence of Cryptosporidium spp . in patients undergoing chemotherapy for malignant solid tumors. SAMPLE SIZE: 94 RESULTS: The prevalence was 2.1% (2/94), 5.3% (5/94), and 5.3% (5/94) as detected by Ziehl–Neelsen staining, real-time PCR and ELISA, respectively. The prevalence reached 8.5% (8/94) using all results obtained from the three methods. Among eight positive stool samples, four were positive by at least two different methods (Ziehl–Neelsen staining-ELISA or ELISA-real-time PCR) whereas the remaining four were positive by either ELISA or real-time PCR. CONCLUSION: These findings show the risk of cryptosporidiosis in cancer patients and the necessity to use at least two diagnostic methods during the diagnosis of cryptosporidiosis to reach more accurate and trustworthy results. LIMITATIONS: Further studies with a larger sample size are recommended. CONFLICT OF INTEREST: None.

scholarly journals Rapid diagnosis of toxinogenic Clostridium difficile in faecal samples with internally controlled real-time PCR

2006 ◽  
Vol 12 (2) ◽  
pp. 184-186 ◽  
Author(s):  
R.J. van den Berg ◽  
E.J. Kuijper ◽  
L.E.S. Bruijnesteijn van Coppenraet ◽  
E.C.J. Claas
Keyword(s):  
Clostridium Difficile ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Diagnosis ◽  
Faecal Samples

PIX-6 Detection of BK-JC viruses in immunocompromised patients using real-time PCR in a university hospital, Ankara, Turkey

2009 ◽  
Vol 46 ◽  
pp. S45
Author(s):  
S. Rota ◽  
K. Fidan ◽  
G. Bozdayi ◽  
A. Dalgic ◽  
I. Fidan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
University Hospital ◽  
Immunocompromised Patients

Real-time PCR is more specific than conventional PCR for induced sputum diagnosis ofPneumocystispneumonia in immunocompromised patients without HIV infection

Respirology ◽  
2009 ◽  
Vol 14 (2) ◽  
pp. 203-209 ◽  
Author(s):  
Tomoyuki FUJISAWA ◽  
Takafumi SUDA ◽  
Hiroyuki MATSUDA ◽  
Naoki INUI ◽  
Yutaro NAKAMURA ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Real Time ◽  
Real Time Pcr ◽  
Induced Sputum ◽  
Immunocompromised Patients ◽  
Conventional Pcr

scholarly journals Prevalence of Dientamoeba fragilis, Giardia duodenalis, Entamoeba histolytica/dispar , and Cryptosporidium spp in Da Nang, Vietnam, detected by a multiplex real‐time PCR

Apmis ◽  
2016 ◽  
Vol 124 (6) ◽  
pp. 529-533 ◽  
Author(s):  
Jessica Ögren ◽  
Song Van Nguyen ◽  
Minh Khac Nguyen ◽  
Jan Dimberg ◽  
Andreas Matussek
Keyword(s):  
Real Time ◽  
Entamoeba Histolytica ◽  
Real Time Pcr ◽  
Giardia Duodenalis ◽  
Dientamoeba Fragilis ◽  
Cryptosporidium Spp
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