AUTOIMMUNE THYROIDITIS: PRESENCE AND ACTIVITY OF HUMAN HERPESVIRUS 6 (HHV-6) INFECTION

2016 ◽  
Author(s):  
Maksims Cistjakovs
Keyword(s):  
Autoimmune Thyroiditis ◽  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Herpesvirus 6

scholarly journals Monocytes/Macrophages Act as Mediators for Human Herpesvirus-6 Infection of Thyroid Gland in Patients with Autoimmune Thyroiditis

2019 ◽  
Vol 73 (2) ◽  
pp. 112-116
Author(s):  
Lība Sokolovska ◽  
Alina Sultanova ◽  
Maksims Čistjakovs ◽  
Egils Cunskis ◽  
Modra Murovska
Keyword(s):  
Thyroid Gland ◽  
Autoimmune Thyroiditis ◽  
Genomic Sequence ◽  
Human Herpesvirus ◽  
Control Group ◽  
Human Herpesvirus 6 ◽  
Active Infection ◽  
Nested Polymerase Chain Reaction ◽  
Peripheral Blood Mononuclear ◽  
Herpesvirus 6

Abstract The aim of this study was to investigate the possibility of using monocytes/macrophages as mediators in human herpesvirus-6 (HHV-6) infection of thyroid gland tissues in autoimmune thyroiditis (AIT). Seventy-three AIT patients were enrolled in this study. The control group consisted of 80 blood donors. Monocyte/macrophage isolation for AIT patient samples was performed by adherence. HHV-6 was detected in peripheral blood mononuclear cell (PBMC) DNA samples using nested polymerase chain reaction (nPCR). Gene expression of HHV-6 active infection marker (U79/80) and chemokine receptors (U12, U51) in patient monocyte/macrophage samples and blood donor PBMC samples was detected using reverse-transcription PCR. HHV-6 viral load was detected by using quantitative-PCR technique. The HHV-6 genomic sequence was found significantly more frequently among AIT patient than control group samples. Markers of active infection were found in 8 AIT patient monocyte/macrophage samples (11%) and in none of control group PBMC samples. HHV-6 U51 mRNA expression was detected only in AIT patient samples (2/24 previously positive for HHV-6). Since HHV-6 genomic sequences were found significantly more frequently in AIT patient samples and active infection markers were found in patient monocytes/macrophages, our results suggest that monocytes/macrophages may be used by HHV-6 as mediators for thyroid gland infection.

scholarly journals Possible Involvement of Human Herpesvirus-6 U83 Gene Expression in Autoimmune Thyroiditis Development

2019 ◽  
Vol 73 (2) ◽  
pp. 78-83
Author(s):  
Alina Sultanova ◽  
Maksims Čistjakovs ◽  
Lība Sokolovska ◽  
Egils Cunskis ◽  
Modra Murovska
Keyword(s):  
Gene Expression ◽  
Thyroid Gland ◽  
Autoimmune Thyroiditis ◽  
Blood Donors ◽  
Human Herpesvirus ◽  
Control Group ◽  
Human Herpesvirus 6 ◽  
Tissue Samples ◽  
Cytokine Levels ◽  
Herpesvirus 6

Abstract Viral infections have been frequently cited as important environmental factors implicated in autoimmune thyroiditis (AIT) development, although no specific virus has yet been conclusively associated with the disease. Some evidence implicates human herpesvirus-6 (HHV-6) in this disease. The aim of this study was to investigate the role of the HHV-6 U83 gene expression in autoimmune thyroiditis development. Fifty-one patients with AIT following thyroidectomy and a control group of 30 autopsied subjects without thyroid pathologies for comparing virology results and 30 healthy blood donors for comparing serology results were enrolled in this study. HHV-6 U83 gene expression was determined using nested PCR with complementary DNA as the template acquired from thyroid gland extracted RNA. Plasma samples of AIT patients and blood donors were tested for IL-2, IL-4, IL-10, sTNF-RII and IL-1beta levels by ELISA. Virology results were compared with pro- and anti-inflammatory cytokine levels to determine possible interaction of HHV-6 with host immune response. HHV-6 U83 gene expression was found only in 24% (12/49) of AIT patient thyroid gland tissue samples and in none of the control group individuals, showing possible involvement of this gene in AIT development. However, no interaction between HHV-6 and changes in cytokine levels was found.

Cytomegalovirus, human herpesvirus-6, and human herpesvirus-7 in hematological patients

2003 ◽  
Vol 40 (2) ◽  
pp. 154-162 ◽  
Author(s):  
A. A. Clark ◽  
C. C. Emery ◽  
D. D. Griffiths
Keyword(s):  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Hematological Patients ◽  
Herpesvirus 6

Leukocytoclastic Vasculitis Associated with HHV6-A/ciHHV6-A and HHV6-B Coinfection in an Immunocompetent Woman

2019 ◽  
Vol 19 (2) ◽  
pp. 221-225 ◽  
Author(s):  
Agata Calvario ◽  
Caterina Foti ◽  
Maria Scarasciulli ◽  
Paolo Romita ◽  
Eva Eliassen ◽  
...  
Keyword(s):  
Transcriptional Activity ◽  
Skin Infection ◽  
Leukocytoclastic Vasculitis ◽  
Human Herpesvirus ◽  
Small Vessel Vasculitis ◽  
Case Description ◽  
Human Herpesvirus 6 ◽  
Late Antigen ◽  
Herpesvirus 6 ◽  
Systemic Symptoms

Background and Objective: Leukocytoclastic vasculitis (LCV) is a small vessel vasculitis that can be limited to the skin but may also affect other organs. Often, its cause is unknown. LCV has previously been reported to occur with the reactivation of human herpesvirus 6 (HHV-6). Here, we report a second instance of HHV-6 reactivation in a 43-year-old woman with idiopathic cutaneous LCV. </P><P> Case Description: In this case, the patient was immunocompetent, and testing revealed that she had inherited chromosomally integrated human herpesvirus 6 variant A (iciHHV6-A) with a parallel skin infection of HHV-6B. The integrated ciHHV-6A strain was found to be transcriptionally active in the blood, while HHV-6B late antigen was detected in a skin biopsy. The patient’s rash was not accompanied by fever nor systemic symptoms and resolved over four weeks without any therapeutic intervention.Conclusion:In light of the transcriptional activity documented in our case, further examination of a possible role for HHV-6 in the etiology of LCV is warranted.

Predominant CD4 T-lymphocyte tropism of human herpesvirus 6-related virus.

1989 ◽  
Vol 63 (7) ◽  
pp. 3161-3163 ◽  
Author(s):  
K Takahashi ◽  
S Sonoda ◽  
K Higashi ◽  
T Kondo ◽  
H Takahashi ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Related Virus ◽  
Herpesvirus 6
Sign in / Sign up

Export Citation Format

Share Document