Effects of low molecular weight heparin on alveolar bone loss in Wistar rats: morphometric and histological analyses

2018 ◽  
Author(s):  
Cassiano Rösing
Keyword(s):  
Molecular Weight ◽  
Bone Loss ◽  
Low Molecular Weight Heparin ◽  
Wistar Rats ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Low Molecular Weight

scholarly journals Effects of Low Molecular Weight Heparin on Alveolar Bone Loss in Wistar Rats

2019 ◽  
Vol 30 (1) ◽  
pp. 12-21
Author(s):  
Harry Juan Rivera Oballe ◽  
Marcelo Lazzaron Lamers ◽  
Francisco Wilker Mustafa Gomes Muniz ◽  
Tobias Rauber Spuldaro ◽  
Eduardo José Gaio ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Bone Loss ◽  
Periodontal Disease ◽  
Low Molecular Weight Heparin ◽  
Wistar Rats ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Low Molecular Weight ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract This study aimed to assess the effects of low molecular weight heparin (LMWH) on alveolar bone loss (ABL), blood count, and counting of megakaryocytes and adipocytes in male Wistar rats. Forty male 60-day Wistar rats were randomly divided into four groups: Control (C), Periodontal Disease (PD), Heparin (Hp) and Heparin + Periodontal Disease (Hp+PD). LMWH was applied for 60 days at doses of 1 ml/kg/day. Blood samples were collected at baseline, 30 and 60. On day-49, PD and Hp+PD groups were subjected to ligature-induced periodontitis around second upper right molar. The left side was assessed as spontaneous alveolar bone loss. Mean ABL in the side with ligature showed significantly different between C (0.35±0.07 mm) and Hp+DP (0.49±0.09 mm) groups (p<0.001), between PD (0.55±0.11 mm) and Hp (0.32±0.06 mm) groups (p<0.001) and between Hp and Hp+DP groups (p<0.001). No significant differences were found among groups for ABL in the side without ligature. Animal weight, food intake, and water consumption showed no statistically significant difference among groups. Megakaryocytes and adipocytes were counted using optical microscopy and no statistically significant differences were found. Within-groups, there were an increase and a decrease, respectively, in the counting of lymphocytes (p=0.005 for C and p=0.009 for Hp+PD groups only) and leukocytes (p=0.003 for C, p=0.001 for PD, p=0.002 for Hp, and p<0.001 for Hp+PD groups). There was no decrease in the number of platelets in the three collection periods. LMWH was not able to affect ABL, but it may change the blood counting, especially increasing lymphocytes.

3,4,5-Trihydroxybenzoic acid attenuates ligature-induced periodontal disease in Wistar rats.

2020 ◽  
Vol 19 ◽  
Author(s):  
Ozkan Karatas ◽  
Fikret Gevrek
Keyword(s):  
Bone Loss ◽  
Gallic Acid ◽  
Wistar Rats ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Collagenase Activity ◽  
Osteoblastic Activity ◽  
Cell Counts ◽  
Experimental Periodontitis ◽  
Anti Inflammatory

Background: 3,4,5-Trihydroxybenzoic acid, which is also known as gallic acid, is an anti-inflammatory agent who could provide beneficial effects in preventing periodontal inflammation. The present study aimed to evaluate the anti-inflammatory effects of gallic acid on experimental periodontitis in Wistar rats. Alveolar bone loss, osteoclastic activity, osteoblastic activity, and collagenase activity were also determined. Methods: 32 Wistar rats were used in the present study. Study groups were created as following: Healthy control (C,n=8) group; periodontitis (P,n=8) group; periodontitis and 30 mg/kg gallic acid administered group (G30,n=8); periodontitis and 60 mg/kg gallic acid administered group (G60,n=8). Experimental periodontitis was created by placing 4-0 silk sutures around the mandibular right first molar tooth. Morphological changes in alveolar bone were determined by stereomicroscopic evaluation. Mandibles were undergone histological evaluation. Matrix metalloproteinase (MMP)-8, tissue inhibitor of MMPs (TIMP)-1, bone morphogenetic protein (BMP)-2 expressions, tartrate-resistant acid phosphatase (TRAP) positive osteoclast cells, osteoblast, and inflammatory cell counts were determined. Results: Highest alveolar bone loss was observed in the periodontitis group. Both doses of gallic acid decreased alveolar bone loss compared to the P group. TRAP-positive osteoclast cell counts were higher in the P group, and gallic acid successfully lowered these counts. Osteoblast cells also increased in gallic acid administered groups. Inflammation in the P group was also higher than those of C, G30, and G60 groups supporting the role of gallic acid in preventing inflammation. 30 and 60 mg/kg doses of gallic acid decreased MMP-8 levels and increased TIMP-1 levels. BMP levels increased in gallic acid administered groups, similar to several osteoblasts. Conclusion: Present results revealed an anti-inflammatory effect of gallic acid, which was indicated by decreased alveolar bone loss and collagenase activity and increased osteoblastic activity.

scholarly journals Effect of inhaled corticosteroid on TNF-α production and alveolar bone loss in Wistar rats

2011 ◽  
Vol 56 (11) ◽  
pp. 1398-1403 ◽  
Author(s):  
Luciana Dondonis Daudt ◽  
Juliano Cavagni ◽  
Eduardo José Gaio ◽  
Andressa Souza ◽  
Iraci Lucena da Silva Torres ◽  
...  
Keyword(s):  
Bone Loss ◽  
Wistar Rats ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Inhaled Corticosteroid ◽  
Tnf Α

Differential effects of heparin and low molecular weight heparin on osteoblastogenesis and adipogenesis in vitro

2004 ◽  
Vol 92 (10) ◽  
pp. 803-810 ◽  
Author(s):  
Stephanie Osip ◽  
Martin Butcher ◽  
Edward Young ◽  
Lufang Yang ◽  
Stephen Shaughnessy
Keyword(s):  
Molecular Weight ◽  
Cell Culture ◽  
Bone Loss ◽  
Culture System ◽  
Low Molecular Weight Heparin ◽  
Low Molecular Weight ◽  
Cell Culture System ◽  
Term Administration ◽  
Stromal Cell Culture

SummaryWe have previously demonstrated that heparin produces cancellous bone loss in rats due in part to a decrease in the number of osteoblasts lining the trabecular bone surface. In the present study, we use a stromal-derived cell culture system together with measurements of alkaline phosphatase (ALP) activity, to compare the effects of heparin and the low molecular weight heparin (LMWH), Fragmin, on osteoblast differentiation in vitro. In addition, we examined the possibility that both heparin and LMWH can induce adipogenesis in our stromal cell culture system. Both heparin and LMWH were found to produce a statistically significant (P <0.01) and concentration-dependent decrease in the number of osteoblasts while increasing the number of adipocytes. When the effects of gravimetrically equivalent amounts of heparin and LMWH were compared, heparin had a 4-fold greater effect than LMWH. In contrast to heparin, N-desulfated heparin was found to have minimal effects on both osteoblast and adipocyte differentiation indicating that the heparin effect is not only chain-length dependent but also charge-dependent. The observation that LMWH has less of an effect on bone formation than heparin is compatible with the results of clinical trials indicating that LMWH produces less bone loss after long-term administration.

Spontaneous alveolar bone loss after 4NQO exposure in Wistar rats

2018 ◽  
Vol 89 ◽  
pp. 44-48
Author(s):  
Harry J.R. Oballe ◽  
Francisco Wilker M.G. Muniz ◽  
Cheyenne C. Bueno ◽  
Isadora P. Klein ◽  
Vinicius C. Carrard ◽  
...  
Keyword(s):  
Bone Loss ◽  
Wistar Rats ◽  
Alveolar Bone ◽  
Alveolar Bone Loss

scholarly journals Effects of Alcohol and/or Tobacco Exposure on Spontaneous Alveolar Bone Loss in Rat

2014 ◽  
Vol 25 (3) ◽  
pp. 197-202 ◽  
Author(s):  
Harry Juan Rivera Oballe ◽  
Eduardo José Gaio ◽  
Tobias Spuldaro ◽  
Juliano Cavagni ◽  
Rosane Gomez ◽  
...  
Keyword(s):  
Body Weight ◽  
Bone Loss ◽  
Wistar Rats ◽  
Alveolar Bone ◽  
Alcohol Exposure ◽  
Alveolar Bone Loss ◽  
Control Group ◽  
Tobacco Exposure ◽  
Periodontal Breakdown ◽  
Group 2

The aim of the present study was to evaluate the effect of alcohol and/or tobacco exposure on spontaneous alveolar bone loss in Wistar rats. Twenty-four, male, 60 day-old, Wistar rats were assigned to 4 groups: Group 1 received 10 mL/kg of glucose solution (5%). Group 2 received 2 g/kg alcohol (20%). Group 3 was exposed to tobacco smoke (6 cigarettes/60 min). Group 4 received both interventions of groups 2 and 3. Alcohol was given by gastric gavage and cigarette exposure was performed using a forced ventilation chamber. After 30 days, animals were sacrificed and the upper maxillae removed and defleshed. Morphometric analysis of alveolar bone loss (ABL) around the second molar was performed in standardized digital photographs. Statistical analysis was conducted using paired t-test, one-way ANOVA and occurrence of spontaneous periodontal disease (ABL ≥ 0.39 mm) was analyzed by Fisher's exact test. Significant differences in body weight were observed between all groups. Group 2 presented higher body weight as compared to the 3 other groups at 4 weeks (p≤0.05). Mean ABL values were 0.31 mm (±0.08), 0.29 mm (±0.07), 0.33 mm (±0.10), and 0.33 mm (±0.08) for groups 1, 2, 3, and 4, respectively. No significant differences were found among groups. In the analysis of occurrence of periodontal breakdown, alcohol exposure decreased the occurrence of ABL and cigarette exposure increased ABL. The combination of alcohol and cigarette exposure did not differ from the control group. Alcohol consumption decreased the occurrence of periodontal breakdown, while tobacco increased this rate.

scholarly journals Low concentration alcohol intake may inhibit spontaneous alveolar bone loss in Wistar rats

2011 ◽  
Vol 56 (2) ◽  
pp. 109-113 ◽  
Author(s):  
Diego Nique Liberman ◽  
Roberta Manjabosco Pilau ◽  
Eduardo José Gaio ◽  
Lorena Floriani Orlandini ◽  
Cassiano Kuchenbecker Rösing
Keyword(s):  
Bone Loss ◽  
Wistar Rats ◽  
Alcohol Intake ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Low Concentration

Quercetin Decreased Alveolar Bone Loss and Apoptosis in Experimentally Induced Periodontitis Model in Wistar Rats

2020 ◽  
Vol 19 (4) ◽  
pp. 436-448
Author(s):  
Mehmet Murat Taskan ◽  
Fikret Gevrek
Keyword(s):  
Bone Loss ◽  
Wistar Rats ◽  
Caspase 3 ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
High Dose ◽  
Tartrate Resistant Acid Phosphatase ◽  
Osteoblastic Activity ◽  
Anti Inflammatory

Background: Quercetin is a flavonoid which has potent anti-inflammatory, antibacterial, and antioxidant effect. Purpose of this study was to evaluate effects of quercetin on alveolar bone loss and histopathological changes in ligature-induced periodontitis in rats. Methods: Wistar rats were divided into four experimental groups: non-ligated control (C, n=8) group; periodontitis (P, n=8) group; ligature and low dose quercetin group (75 mg/kg/day quercetin, Q75 group, n=8); ligature and high dose quercetin group (150 mg/kg/day quercetin, Q150 group, n=8). Silk ligatures were placed at gingival margin of lower first molars of mandibular right quadrant. Study duration was 15 days, and animals were sacrificed end of this period. Changes in alveolar bone levels were clinically measured and tissues were immunohistochemically examined, matrix metalloproteinase 8 (MMP 8), inducible nitric oxide synthase (iNOS), tissue inhibitor of metalloproteinase 1 (TIMP 1), Cysteine-aspartic proteases 3 (Caspase 3), and tartrate-resistant acid phosphatase (TRAP) positive osteoclast cells, osteoblast, and neutrophil counts were also determined. Results and Discussion: Alveolar bone loss was highest in P group, and differences among P, Q75, and Q150 groups were significant. Both doses of quercetin decreased TRAP+ osteoclast cells and increased osteoblast cells. Inflammation in P group was also higher than those of C, Q75, and Q150 groups indicating anti-inflammatory effect of quercetin. iNOS, MMP-8, and caspase-3 levels were highest, and TIMP-1 expression was lowest in P group; differences were statistically significant. Conclusion: Within limits of this study, it can be suggested that quercetin administration may reduce alveolar bone loss by increasing osteoblastic activity, decreasing osteoclastic activity, apoptosis, and inflammation in an experimental model of periodontitis.

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