0935 - Simultaneous selection of soil electroactive bacterial communities associated to anode and cathode in a two-chamber Microbial Fuel Cell

Abstract. Khoirunnisa NS, Anwar S, Santosa DA. 2020. Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell. Biodiversitas 21: 1686-1696. Cellulose such as in rice straw can be utilized as an organic substrate in Microbial Fuel Cell (MFC) to generate electricity by microorganisms as a biocatalyst. This research aimed to get cellulose-degrading bacteria with high capability to degrade rice straw and able to be used as consortium with exoelectrogen bacteria in Microbial Fuel Cell. The stages of research included: (i) isolation of the bacteria using carboxymethylcellulose (CMC) agar medium, (ii) selection of the isolates for that purpose, (iii) enzyme assay and MFC performance test, and (iv) identification of selected isolate. There were 125 isolates that were obtained. Selection based on the ability to degrade cellulose as indicated by clear zone on CMC medium resulted in 23 isolates. Ten isolates belong to anaerobic facultative bacteria were selected. Three of them were synergistic with exoelectrogen bacteria. The three isolates were tested for exoglucanase (Avicel) and total enzyme activity (Filter Paper) with the highest results were 6.21 U/mL (isolate J404) and 5.88 U/mL (isolate J401), respectively. The optimum MFC performance was achieved by one isolate, J401, which produced highest voltage of 40.8 mV and a power density of 0.33 mW/m2. The best isolate, J401, was identified as Xanthomonas translucens based on 16S rRNA method.

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Improvement of Microbial Fuel Cell Performance by Selection of Anodic Materials and Enrichment of Inoculum

Journal of New Materials for Electrochemical Systems ◽

10.14447/jnmes.v18i3.357 ◽

2015 ◽

Vol 18 (3) ◽

pp. 121-129 ◽

Cited By ~ 7

Author(s):

G. Hernández-Flores ◽

H. M. Poggi-Varaldo ◽

O. Solorza-Feria ◽

M. T. Ponce Noyola ◽

T. Romero-Castanón ◽

...

Keyword(s):

Mathematical Model ◽

Activated Carbon ◽

Fuel Cell ◽

Microbial Fuel Cell ◽

Internal Resistance ◽

Fuel Cell Performance ◽

Sulfate Reducing ◽

Electricity Power ◽

Graphite Rod ◽

Selection Of

This work aimed at evaluating the effect of four anodic materials and the use of enriched inocula on the microbial fuel cell (MFC) performance. The anodic materials were granular activated carbon (GAC), graphite rod (GR), triangles of graphite (GT) and graphite flakes (GF). When loaded with a sulfate-reducing inoculum (SR-In) the internal resistance (Rint) obtained were 273, 410 and 795 Ω for GF, GT, GR, respectively and higher than 10 000 Ω for GAC, whereas the maximum volumetric power (PV,max) were 1326, 2108 and 3052 mW m-3 for GR, GT and GF, respectively. We observed a decrease of Rint and an increase of PV,max with the increase of the log of A´s of the graphite anodic materials that was consistent with a mathematical model previously reported by our Group. The use of the Fe (III)-reducing inoculum significantly enhanced the MFC performance; PV,max was up to 5000 mW m-3, 40% higher than the power obtained with SR-In whereas the Rint was 140 ohms. Highest PVs of our MFC were close to values of electricity power derived from the anaerobic digestion of municipal wastewaters. In this regard, results of this work point out to a promising approach to further tapping bioelectricity from organic wastes that previously have yielded biohydrogen.

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