Bifunctional Small Molecules That Mediate the Degradation of Extracellular Proteins
<p>Targeted protein degradation (TPD) has emerged as a promising and exciting therapeutic strategy. The majority of existing TPD technologies rely on the ubiquitin-proteasome system, and are therefore limited to targeting intracellular proteins. To address this limitation, we developed a class of modularly designed, bifunctional synthetic molecules called <b>MoDE-A</b>s (<b>Mo</b>lecular <b>D</b>egraders of <b>E</b>xtracellular proteins through the <b>A</b>sialoglycoprotein receptor (ASGPR)), which are capable of mediating the degradation of extracellular proteins. MoDE-A molecules mediate the formation of a ternary complex between a target protein and the ASGPR, which is expressed primarily on hepatocytes. The target protein is then endocytosed and degraded by lysosomal proteases. We demonstrated the modularity of the MoDE-A technology by synthesizing bifunctional molecules that induce the degradation of both antibody and pro-inflammatory cytokine proteins. To our knowledge, these data represent the first experimental evidence that non-proteinogenic, synthetic molecules can be employed for the TPD of extracellular proteins both <i>in vitro</i> and <i>in vivo</i>. We believe that TPD mediated by the MoDE-A technology will have widespread applications for disease treatment.</p>