Molecular Mapping of Neutral Lipids using Silicon Nanopost Arrays and TIMS Imaging Mass Spectrometry

<p>Our work demonstrates the ability to perform high spatial resolution imaging mass spectrometry (IMS) using silicon nanopost arrays (NAPA), a matrix-free laser desorption ionization (LDI) substrate, for multiple classes of neutral lipids from biological tissue sections.</p> <p><br></p><p>Utilized trapped ion-mobility spectrometry (TIMS) coupled with IMS to enhance the molecular coverage of detected neutral lipid species, as well as for differentiating neutral lipid isomeric species spatial distributions. </p>