Typical errors in the preanalytical phase of laboratory

2017 ◽  
pp. 84-90
Author(s):  
O.V. Brazhnikova ◽  
◽  
N.V. Gavelya ◽  
I.D. Maykova ◽  
◽  
...  
Keyword(s):  
Preanalytical Phase

scholarly journals Preanalytical Phase in Haematology

2008 ◽  
Vol 27 (3) ◽  
pp. 348-353 ◽  
Author(s):  
Giuseppe Banfi ◽  
L. Germagnoli
Keyword(s):  
Clinical Decision ◽  
Blood Parasites ◽  
Haematological Parameters ◽  
Pathological Conditions ◽  
Blood Drawing ◽  
Preanalytical Phase ◽  
Valid Data ◽  
Spurious Results ◽  
Instrumental Precision ◽  
Hematological Tests

Preanalytical Phase in Haematology The preanalytical phase is particularly important in haematology, where counts of particles and cells are performed in whole anticoagulated blood. The correct use and concentration of anticoagulant is mandatory to avoid spurious results, which can influence clinical decision. EDTA is the anticoagulant of choice, but it has some limits, especially for preserving stability and shape of platelets. Stability of haematological parameters is high, with the exception of leukocytes and reticulocytes. However, stability (and instrumental precision) should be evaluated together with biological variability and individuality index of various haematological parameters. Hematological tests are also influenced and interfered by high amounts of lipids and chylomicrons. The mixing procedure of the tubes after blood drawing and before analysis is also crucial for obtaining correct and valid data. There are some examples of interferences on automated haematological analysers which are used for diagnosing and screening pathological conditions. Cryoglobulins and erythrocytes parasites can induce spurious results of WBC, RBC and PLT, but the repeatability of these interferences could be used for alerting the pathologist and could reveal the presence of pathological proteins or blood parasites. New parameters have been proposed by modern haematological analysers, directly defined or calculated from traditional measures, but the clinical impact of these new parameters is often dependent on preanalytical variables.

scholarly journals An important source of preanalytical error in medical laboratories: centrifugation

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Cigdem Sonmez ◽  
Alper Gümüş ◽  
Mehmet Senes ◽  
Guzin Aykal ◽  
Fatma Taneli ◽  
...  
Keyword(s):  
Centrifugal Force ◽  
Gravitational Acceleration ◽  
Medical Laboratories ◽  
Electric Device ◽  
Preanalytical Phase ◽  
Plasma And Urine Samples ◽  
Relative Centrifugal Force ◽  
Technical Specifications ◽  
Physical Impact ◽  
Rotor Type

Abstract Centrifugation separates particles within the specimen according to their shape, dimensions, and density and basically can be defined as a separation method. The centrifuge is an essential device in medical laboratories to prepare the serum, plasma, and urine samples for analysis. It is basically an electric device composed of the stationary (motor) and the motile (rotor) part. The centrifugation depends on two main variables: relative centrifugal force (RCF) and centrifugation time. The physical impact separating the specimen into its components in the centrifuge known as RCF is expressed as the multiples of gravitational acceleration (×g). RPM, defined as the number of rotations of the centrifuge per minute, shows the speed of the centrifuge. RCF value can be calculated by using RPM, and the centrifuge radius. Because models and sizes of centrifuges vary considerably, the use of gravity (g) forces instead of RPM is suggested. The centrifuges can be classified according to their usage, speed, technical specifications, and rotor type. An accurate and precise centrifugation process is essential to prevent errors in the preanalytical phase. The purpose of this document is to ensure the standardization of a good, precise protocol for the centrifugation process among the medical laboratories.

scholarly journals The Effects of Education and Training Given to Phlebotomists for Reducing Preanalytical Errors

2018 ◽  
Vol 37 (2) ◽  
pp. 172-180 ◽  
Author(s):  
Fatma Demet Arslan ◽  
Inanc Karakoyun ◽  
Banu Isbilen Basok ◽  
Merve Zeytinli Aksit ◽  
Esma Celik ◽  
...  
Keyword(s):  
Correct Answer ◽  
Health Professionals ◽  
Error Rates ◽  
Health Practice ◽  
Sample Collection ◽  
Nursing Science ◽  
Preanalytical Phase ◽  
Before And After ◽  
Patient Preparation ◽  
And Training

SummaryBackground:The most common sources of error in the preanalytical phase are considered to be at the stage of patient preparation and sample collection. In order to reduce the preanalytical errors, we aimed to determine the level of phlebotomists knowledge about the preanalytic phase before and after planned trainings in the study.Methods:Training about preanalytical processes was given to the 454 health professionals and the majority of them were employed as nurse. Questionnaires before and after training were conducted. In order to assess the effect of the training into the process, preanalytical error rates were calculated before and after training.Results:The total correct answer rates of vocational school of health diplomaed were statistically lower than the total correct answer rates of other. It was observed significantly increase in the rate of correct answers to questionnaire and significantly decrease in preanalytical error rates after training.Conclusions:The results of the survey showed that the attitudes of the phlebotomists were diverse in the preanalytical processes according to the levels of education and their practices. By providing training to all staff on a regular basis, their information about preanalytical phase could be updated and hence, it may possible to significantly reduce the preanalytical errors in health practice and nursing science.

The preanalytical phase – a field for improvement

Diagnosis ◽  
2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Alexander von Meyer ◽  
Janne Cadamuro
Keyword(s):  
Preanalytical Phase

scholarly journals CRYOGLOBULIN TEST AND CRYOGLOBULINEMIA HEPATITIS C-VIRUS RELATED

2016 ◽  
Vol 9 (1) ◽  
pp. e2017007 ◽  
Author(s):  
Umberto Basile
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Confounding Factor ◽  
Underlying Disease ◽  
Clinical Syndrome ◽  
Laboratory Techniques ◽  
Preanalytical Phase ◽  
Wide Range

Cryoglobulins are immunoglobulins that precipitate in serum at temperatures below 37°C and resolubilize upon warming. The clinical syndrome of cryoglobulinemia usually includes purpura, weakness, and arthralgia, but the underlying disease may also contribute other symptoms. Blood samples for cryoglobulin are collected, transported, clotted and spun at 37°C, before the precipitate is allowed to form when serum is stored at 4°C in a Wintrobe tube for at least seven days. The most critical and confounding factor affecting the cryoglobulin test is when the preanalytical phase is not fully completed at 37°C. The easiest way to quantify cryoglobulins is the cryocrit estimate. However, this approach has low accuracy and sensitivity. Furthermore, the precipitate should be resolubilized by warming to confirm that it is truly formed of cryoglobulins. The characterization of cryoglobulins requires the precipitate is several times washed, before performing immunofixation, a technique by which cryoglobulins can be classified depending on the characteristics of the detected immunoglobulins. These features imply a pathogenic role of these molecules which are consequently associated with a wide range of symptoms and manifestations. According to the Brouet classification, Cryoglobulins are grouped into three types by the immunochemical properties of immunoglobulins in the cryoprecipitate. The aim of this paper is to review the major aspects of cryoglobulinemia and the laboratory techniques used to detect and characterize cryoglobulins, taking into consideration the presence and consequences of cryoglobulinemia in Hepatitis C Virus (HCV) infection.

scholarly journals Implementation of a two-dimensional barcode in the preanalytic phase

2004 ◽  
Vol 23 (3) ◽  
pp. 317-320 ◽  
Author(s):  
Cyrill Müller ◽  
Joachim Singer
Keyword(s):  
Clinical Data ◽  
Data Transmission ◽  
Laboratory Data ◽  
Two Dimensional ◽  
Central Laboratory ◽  
Laboratory Methods ◽  
Preanalytical Phase ◽  
Data Transmission System ◽  
Near Future ◽  
Secure Transmission

The automation process in the preanalytic phase of the laboratory is of increasing interest since there is an increasing discrepancy between the enormous advances in the automation process of the analytical/ postanalytical and the preanalytical phase of the central laboratory. Besides there is need of a secure transmission of the test requests from the clients to the central laboratory. We started a project together with other supra-regional laboratories in Germany in order to develop and establish a secure and robust data transmission system for the transmission of clinical data and laboratory information to the central laboratory. Based on common standards for laboratory data transmission and using a two dimensional barcode we developed an offline usable open source standard. Our project and the members of our project group may be a basis for the development and establishment of a standardized catalogue of laboratory methods and laboratory analyses in Germany in the near future.

Medical Staff Training - Quality Initiative to Reduce Errors in the Pre-Preanalytical Phase

2021 ◽  
Vol 67 (01/2021) ◽  
Author(s):  
Carmen Delianu ◽  
Loredana Hurjui ◽  
Claudia Tărniceriu ◽  
Mihaela Blaj ◽  
Camelia Tamaș ◽  
...  
Keyword(s):  
Staff Training ◽  
Medical Staff ◽  
Preanalytical Phase ◽  
Training Quality
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