Involvement of Anti-Inflammatory Mechanism in the Antidepressant Activity of Methanol Stem Bark Extract of Adansonia digitata L. (Malvaceae)

2019 ◽  
Vol 3 (2) ◽  
pp. 54-57
Author(s):  
Aishatu Shehu ◽  
◽  
Sagir Mustapha ◽  
Isah Mansir ◽  
Mohammed Magaji
Keyword(s):  
Antidepressant Activity ◽  
Stem Bark ◽  
Bark Extract ◽  
Adansonia Digitata ◽  
Inflammatory Mechanism ◽  
Stem Bark Extract ◽  
Anti Inflammatory

scholarly journals Antidepressant effect of methanol stem bark extract of Adansonia digitata: involvement of monoaminergic, nitric oxide and cholinergic pathways

2020 ◽  
Vol 10 (1) ◽  
pp. 84-92
Author(s):  
Aishatu Shehu ◽  
Mohammed Garba Magaji ◽  
Jamilu Yau ◽  
Abubakar Ahmed
Keyword(s):  
Nitric Oxide ◽  
Receptor Antagonist ◽  
Antidepressant Activity ◽  
Stem Bark ◽  
Bark Extract ◽  
Antidepressant Effect ◽  
Adansonia Digitata ◽  
Receptor Antagonists ◽  
Cholinergic Pathways ◽  
Stem Bark Extract

Introduction: Hausa people of north-western Nigeria were reported to utilize the plant Adansonia digitata for the management of depressive illnesses in an ethnobotanical survey. Thus, this study aimed to establish the mechanism(s) via which methanol stem bark extract of A. digitata (MEAD) exhibits antidepressant activity in mice. Methods: Antidepressant activity of MEAD was evaluated using tail suspension test (TST) at doses of 250, 500 and 1000 mg/kg. For the mechanistic studies, mice were pre-treated with sulpiride (50 mg/kg), prazosin (1 mg/kg), yohimbine (1 mg/kg), metergoline (1 mg/kg), cyproheptadine (3 mg/kg), L-arginine (50 mg/kg), N omega-nitro-L-arginine (L-NNA; 50 mg/kg), atropine (1 mg/kg) and naloxone (2 mg/kg) 15 minutes prior to MEAD (1000 mg/kg) administration, then antidepressant activity was assessed using TST one hour later. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc test. Results: The extract (at doses of 250, 500 and 1000 mg/kg) significantly (P < 0.05) and dose-dependently decreased the duration of immobility in the TST. Sulpiride (D2 receptor antagonist), prazosin and yohimbine (α1 and α2 receptor antagonists, respectively), metergoline and cyproheptadine (5-HT1 and 5-HT2 receptor antagonists, respectively) significantly (P < 0.05) reversed the antidepressant effect of MEAD. On the other hand, L-NNA (NOS inhibitor) augmented the antidepressant effect of MEAD while L-arginine (nitric oxide substrate) had no effect on MEAD. However, atropine (muscarinic receptor antagonist) significantly (P < 0.01) augmented the antidepressant effect of MEAD. Conclusion: The antidepressant activity of methanol stem bark extract of A. digitata was established to be via the monoaminergic, nitric oxide and cholinergic pathways.

Anti-inflammatory and Analgesic Properties of Ethanolic Stem Bark Extract of Ficus trichopoda in Rats.

2011 ◽  
Vol 2 (18) ◽  
pp. 43-47
Author(s):  
Abayomi M Ajayi ◽  
Julius K Tanayena ◽  
Sikiru O Balogun ◽  
Aminu Ibrahim ◽  
Joseph OC Ezeonwumelu ◽  
...  
Keyword(s):  
Stem Bark ◽  
Bark Extract ◽  
Stem Bark Extract ◽  
Anti Inflammatory

scholarly journals Macaranga barteri stem bark extract exerts anti-inflammatory and anti-hyperalgesia activity in murine models

2019 ◽  
Vol 6 (3) ◽  
Author(s):  
Evelyn Asante-Kwatia ◽  
Yakubu Jibira ◽  
Abraham Yeboah Mensah ◽  
Debora Osei-Sarfoh
Keyword(s):  
Stem Bark ◽  
Bark Extract ◽  
Murine Models ◽  
Stem Bark Extract ◽  
Anti Inflammatory

scholarly journals The Ethanolic Stem-Bark Extract of Antrocaryon micraster Inhibits Carrageenan-Induced Pleurisy and Pedal Oedema in Murine Models of Inflammation

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Leslie B. Essel ◽  
David D. Obiri ◽  
Newman Osafo ◽  
Aaron O. Antwi ◽  
Babatunde M. Duduyemi
Keyword(s):  
Acute Toxicity ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Tissue Level ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Murine Models ◽  
Paw Oedema ◽  
Stem Bark Extract ◽  
Anti Inflammatory

We investigated the antioxidant and anti-inflammatory effects of a 70% v/v ethanol extract of the stem bark of Antrocaryon micraster on murine models of carrageenan-induced pleurisy and paw oedema. Rat pleural fluid was analysed for volume, protein content, and leucocytes, while lung histology was assessed for damage. Lung tissue homogenates were assayed for glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and myeloperoxidase (MPO). Phytochemical analysis was carried out on the stem bark. Acute toxicity studies were conducted in rats. In the pleurisy model the extract (30–300 mg/kg) significantly reduced the volume and amount of proteins and leucocytes in the exudate and also protected against lung injury. Tissue level of GSH and SOD and CAT expression were increased while MDA level and MPO activity were reduced. The peak and total oedema responses were significantly suppressed when given both preemptively and curatively in the mice paw oedema test. Saponins, alkaloids, triterpenoids, and tannins were present in the stem bark. A. micraster extract exhibited no apparent acute toxicity. We conclude that the ethanolic stem-bark extract of A. micraster has antioxidant action and exhibits significant anti-inflammatory activity through suppression of pleurisy and paw oedema induced with carrageenan.

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF KNEMA ATTENUATA (HOOK. F. AND THOMSON) WARB ETHANOLIC STEM BARK EXTRACT IN ALBINO WISTAR RATS

2019 ◽  
pp. 330-334
Author(s):  
SUPRIYA RAJA H
Keyword(s):  
Stem Bark ◽  
Inflammatory Activity ◽  
Assay Method ◽  
Bark Extract ◽  
Raw 264.7 ◽  
Myeloperoxidase Activity ◽  
Stem Bark Extract ◽  
Anti Inflammatory

Objective: Knema attenuata (Myristicaceae), popularly known as “wild nutmeg,” is an endemic tree species from Western Ghats, which has been used in folk medicine. Conventionally, the stem bark of K. attenuata is used for treating inflammatory conditions without any scientific information available for the same. The present study was undertaken to evaluate the anti-inflammatory activity of the ethanolic stem bark extract (ESBE) of K. attenuata using in vivo and in vitro screening models. Methods: The ethanolic extract of stem bark was prepared by soxhlation, and its cytotoxicity in RAW 264.7 cell line was assessed using MTT assay method. In vivo anti-inflammatory effect of extract was estimated in rats using carrageenan-induced paw edema model and cotton pellet-induced granuloma model. The in vitro anti-inflammatory activity of the extract was evaluated by cyclooxygenase and lipoxygenase inhibition assay, estimation of myeloperoxidase activity, and determination of cellular nitrite levels in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Results: Toxic symptoms were not observed for the ESBE. The extract demonstrated significant anti-inflammatory activity in both in vivo and in vitro models. The anti-inflammatory action exhibited by the extract was a result of the inhibition of leukocyte migration and nitric oxide pathway and partially by inhibition of mediators such as prostaglandins and leukotrienes. Conclusion: Findings from the study provide the evidence for the popular use of stem bark extract of K. attenuata as a potential anti-inflammatory agent.

Antioxidant anti-inflammatory activity of Alstonia scholaris R.Br. stem bark extract

2012 ◽  
Vol 2 (2) ◽  
pp. 55-57 ◽  
Author(s):  
Chandrashekar Kodangala Subraya ◽  
Harikiran ◽  
Daksha Gupta
Keyword(s):  
Stem Bark ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Alstonia Scholaris ◽  
Stem Bark Extract ◽  
Anti Inflammatory

scholarly journals Antinociceptive and Anti-Inflammatory Activities of Ethanolic Stem Bark Extract of Smilax zeylanica Linn. in Wistar Rats

2021 ◽  
pp. 103-113
Author(s):  
Rasheed Ahemad Shaik ◽  
Fazil Ahmad ◽  
Mohammed Abdul Aleem
Keyword(s):  
Acetic Acid ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Drug Group ◽  
Bark Extract ◽  
Hot Plate ◽  
Paw Oedema ◽  
Stem Bark Extract ◽  
Anti Inflammatory

Background: Smilax zeylanica Linn (Smilaceae) is a woody climbing shrub native to Asia's tropical and subtropical highlands. Traditionally, this plant has been widely used to cure a variety of ailments. Smilax zeylanica stem bark was extracted with ethanol to test its antinociceptive and anti-inflammatory properties. Objective: To study the antinociceptive and anti-inflammatory activities of ethanolic stem bark extract of Smilax zeylanica Linn. in Wistar rats. Methods: The antinociceptive activity has been assessed in mice by formalin-induced paw licking, Eddy’s hot-plate, and acetic-acid induced abdominal stretching models. Carrageenan-induced paw oedema in rats has been conducted to explore the anti-inflammatory activity. Tween 80 (10ml/kg, p.o) was given to the control group, test drug group received ethanol extract of Smilax zeylanica (EESZ) orally at doses of 200 and 400 mg/kg, and standard drugs morphine (5 mg/kg, s.c), diclofenac sodium (10 mg/kg, i.p) were injected to the standard drug group. Results: Oral administration of ethanolic extract of Smilax zeylanica (EESZ) at test doses (200 and 400 mg/kg), produced a significant decrease of the paw licking time for 2 phases (neurogenic and inflammatory) in the formalin test, upsurge of the reaction time in Eddy’s hot plate test, and significantly decreased the abdominal stretching in the acetic acid-induced  writhes test. Ethanol extract of Smilax zeylanica significantly decreased the carrageenan-induced paw oedema. Conclusion: The ethanol extract of Smilax zeylanica possesses antinociceptive, anti-inflammatory activity, probably involving central and peripheral pathways

Evaluation of the Anti-inflammatory Properties ofChlorophora excelsa. Stem Bark Extract

2005 ◽  
Vol 43 (9) ◽  
pp. 746-748 ◽  
Author(s):  
Olumayokun A. Olajide ◽  
Oyetunji T. Kolawole ◽  
Temitope R. Fagbohun ◽  
Franklin F. Ajayi
Keyword(s):  
Stem Bark ◽  
Bark Extract ◽  
Stem Bark Extract ◽  
Anti Inflammatory

Antinociceptive and anti-inflammatory effects of the methanolic stem bark extract of Antrocaryon klaineanum Pierre (Anacardiaceae) in mice and rat

2017 ◽  
Vol 203 ◽  
pp. 11-19 ◽  
Author(s):  
Annie Laure Magne Fongang ◽  
Edwige Laure Nguemfo ◽  
Yolande Djouatsa Nangue ◽  
Calvin Bogning Zangueu ◽  
Yannick Fouokeng ◽  
...  
Keyword(s):  
Stem Bark ◽  
Bark Extract ◽  
Stem Bark Extract ◽  
Anti Inflammatory

scholarly journals The Hydroethanolic Stem Bark Extract of Tieghemella heckelii (A.Chev.) Pierre ex Dubard (Sapotaceae) Produced N-Methyl-D-Aspartate (NMDA) Receptor-Dependent Analgesia and Attenuates Acute Inflammatory Pain via Disruption of Oxidative Stress

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Emmanuel K. Kumatia ◽  
Regina Appiah-Opong
Keyword(s):  
Oxidative Stress ◽  
Nmda Receptor ◽  
Stem Bark ◽  
Bark Extract ◽  
Analgesic Action ◽  
Hot Plate ◽  
Phytochemical Composition ◽  
Stem Bark Extract ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Background. Tieghemella heckelii stem bark is used in African traditional medicine to treat inflammatory pain conditions. However, these biological actions of the plant have not been proven. This study investigates the phytochemical composition and the mechanisms of analgesic and anti-inflammatory actions of the hydroethanolic stem bark extract of T. heckelii (THBE). Methods. Phytochemical composition of THBE was investigated using qualitative and quantitative phytochemical analyses. Anti-inflammatory activity was evaluated using the carrageenan-induced paw oedema assay. Analgesic activity was evaluated using hot plate and acetic acid-induced writhing assays. Mechanism of analgesic action was determined using pharmacological antagonist such as naloxone, atropine, flumazenil, nifedipine, or ketamine. Test agents were administered orally as follows: Tween 80 (5%) (control), diclofenac sodium (DS) 10/tramadol 9 mg/kg (standard), or THBE 10, 100, and 450 mg/kg. Glutathione peroxidase (GPx), superoxide dismutase (SOD), and lipid peroxidation levels were also measured. Results. THBE which contained 58.45% saponins, 229.04 ± 0.049 GAE mg/g phenolic compounds,and 0.482 ± 0.0028 QE mg/g flavonoids produced ( p  < 0.5) anti-inflammatory effect of 56.22% and analgesia of 330 ± 72% and 50.4% in the hot plate and writhing assays, respectively, at 10 mg/kg and inhibited oxidative stress by GPx and SOD elevation in rats during inflammation. Ketamine significantly blocked the analgesia of THBE, indicating NMDA receptor-dependent analgesic action. Whereas, naloxone, atropine, nifedipine, and flumazenil could not antagonize the analgesic action of THBE. Conclusion. These results show that THBE produced potent anti-inflammatory effect via disruption of oxidative stress and also generated NMDA receptor-dependent analgesia.

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