Antioxidant anti-inflammatory activity of Alstonia scholaris R.Br. stem bark extract

2012 ◽  
Vol 2 (2) ◽  
pp. 55-57 ◽  
Author(s):  
Chandrashekar Kodangala Subraya ◽  
Harikiran ◽  
Daksha Gupta
Keyword(s):  
Stem Bark ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Alstonia Scholaris ◽  
Stem Bark Extract ◽  
Anti Inflammatory

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF KNEMA ATTENUATA (HOOK. F. AND THOMSON) WARB ETHANOLIC STEM BARK EXTRACT IN ALBINO WISTAR RATS

2019 ◽  
pp. 330-334
Author(s):  
SUPRIYA RAJA H
Keyword(s):  
Stem Bark ◽  
Inflammatory Activity ◽  
Assay Method ◽  
Bark Extract ◽  
Raw 264.7 ◽  
Myeloperoxidase Activity ◽  
Stem Bark Extract ◽  
Anti Inflammatory

Objective: Knema attenuata (Myristicaceae), popularly known as “wild nutmeg,” is an endemic tree species from Western Ghats, which has been used in folk medicine. Conventionally, the stem bark of K. attenuata is used for treating inflammatory conditions without any scientific information available for the same. The present study was undertaken to evaluate the anti-inflammatory activity of the ethanolic stem bark extract (ESBE) of K. attenuata using in vivo and in vitro screening models. Methods: The ethanolic extract of stem bark was prepared by soxhlation, and its cytotoxicity in RAW 264.7 cell line was assessed using MTT assay method. In vivo anti-inflammatory effect of extract was estimated in rats using carrageenan-induced paw edema model and cotton pellet-induced granuloma model. The in vitro anti-inflammatory activity of the extract was evaluated by cyclooxygenase and lipoxygenase inhibition assay, estimation of myeloperoxidase activity, and determination of cellular nitrite levels in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Results: Toxic symptoms were not observed for the ESBE. The extract demonstrated significant anti-inflammatory activity in both in vivo and in vitro models. The anti-inflammatory action exhibited by the extract was a result of the inhibition of leukocyte migration and nitric oxide pathway and partially by inhibition of mediators such as prostaglandins and leukotrienes. Conclusion: Findings from the study provide the evidence for the popular use of stem bark extract of K. attenuata as a potential anti-inflammatory agent.

scholarly journals Nanoemulsion as a carrier to improve the topical anti-inflammatory activity of stem bark extract of Rapanea ferruginea

2016 ◽  
Vol Volume 11 ◽  
pp. 4495-4507 ◽  
Author(s):  
Tania M B Bresolin ◽  
Juarana Dal Mas ◽  
Tailyn Zermiani ◽  
Liliani Thiesen ◽  
Joana L. M. Silveira ◽  
...  
Keyword(s):  
Stem Bark ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Stem Bark Extract ◽  
Anti Inflammatory

scholarly journals The anti-inflammatory activity of standard aqueous stem bark extract of Mangifera indica L. as evident in inhibition of Group IA sPLA2

2016 ◽  
Vol 88 (1) ◽  
pp. 197-209 ◽  
Author(s):  
BHADRAPURA LAKKAPPA DHANANJAYA ◽  
SUDHARSHAN SHIVALINGAIAH
Keyword(s):  
Mangifera Indica ◽  
Direct Interaction ◽  
Pharmaceutical Formulations ◽  
Stem Bark ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Binding Studies ◽  
Edema Formation ◽  
Stem Bark Extract ◽  
Anti Inflammatory

ABSTRACT The standard aqueous stem bark extract is consumed as herbal drink and used in the pharmaceutical formulations to treat patients suffering from various disease conditions in Cuba. This study was carried out to evaluate the modulatory effect of standard aqueous bark extract of M. indica on Group IA sPLA2. M. indica extract, dose dependently inhibited the GIA sPLA2 (NN-XIa-PLA2) activity with an IC50 value 8.1 µg/ml. M. indica extract effectively inhibited the indirect hemolytic activity up to 98% at ~40 µg/ml concentration and at various concentrations (0-50 µg/ml), it dose dependently inhibited the edema formation. When examined as a function of increased substrate and calcium concentration, there was no relieve of inhibitory effect on the GIA sPLA2. Furthermore, the inhibition was irreversible as evidenced from binding studies. It is observed that the aqueous extract ofM. indica effectively inhibits sPLA2 and it is associated inflammatory activities, which substantiate their anti-inflammatory properties. The mode of inhibition could be due to direct interaction of components present in the extract, with sPLA2 enzyme. Further studies on understanding the principal constituents, responsible for the anti-inflammatory activity would be interesting to develop this into potent anti-inflammatory agent.

Anti-inflammatory and Analgesic Properties of Ethanolic Stem Bark Extract of Ficus trichopoda in Rats.

2011 ◽  
Vol 2 (18) ◽  
pp. 43-47
Author(s):  
Abayomi M Ajayi ◽  
Julius K Tanayena ◽  
Sikiru O Balogun ◽  
Aminu Ibrahim ◽  
Joseph OC Ezeonwumelu ◽  
...  
Keyword(s):  
Stem Bark ◽  
Bark Extract ◽  
Stem Bark Extract ◽  
Anti Inflammatory

scholarly journals Macaranga barteri stem bark extract exerts anti-inflammatory and anti-hyperalgesia activity in murine models

2019 ◽  
Vol 6 (3) ◽  
Author(s):  
Evelyn Asante-Kwatia ◽  
Yakubu Jibira ◽  
Abraham Yeboah Mensah ◽  
Debora Osei-Sarfoh
Keyword(s):  
Stem Bark ◽  
Bark Extract ◽  
Murine Models ◽  
Stem Bark Extract ◽  
Anti Inflammatory

scholarly journals The Ethanolic Stem-Bark Extract of Antrocaryon micraster Inhibits Carrageenan-Induced Pleurisy and Pedal Oedema in Murine Models of Inflammation

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Leslie B. Essel ◽  
David D. Obiri ◽  
Newman Osafo ◽  
Aaron O. Antwi ◽  
Babatunde M. Duduyemi
Keyword(s):  
Acute Toxicity ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Tissue Level ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Murine Models ◽  
Paw Oedema ◽  
Stem Bark Extract ◽  
Anti Inflammatory

We investigated the antioxidant and anti-inflammatory effects of a 70% v/v ethanol extract of the stem bark of Antrocaryon micraster on murine models of carrageenan-induced pleurisy and paw oedema. Rat pleural fluid was analysed for volume, protein content, and leucocytes, while lung histology was assessed for damage. Lung tissue homogenates were assayed for glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and myeloperoxidase (MPO). Phytochemical analysis was carried out on the stem bark. Acute toxicity studies were conducted in rats. In the pleurisy model the extract (30–300 mg/kg) significantly reduced the volume and amount of proteins and leucocytes in the exudate and also protected against lung injury. Tissue level of GSH and SOD and CAT expression were increased while MDA level and MPO activity were reduced. The peak and total oedema responses were significantly suppressed when given both preemptively and curatively in the mice paw oedema test. Saponins, alkaloids, triterpenoids, and tannins were present in the stem bark. A. micraster extract exhibited no apparent acute toxicity. We conclude that the ethanolic stem-bark extract of A. micraster has antioxidant action and exhibits significant anti-inflammatory activity through suppression of pleurisy and paw oedema induced with carrageenan.

scholarly journals Comparative Study of Antimicrobial, Anti-Inflammatory, and Antioxidant Activities of Different Parts from Pterocarpus Santalinoides l’Her. Ex. DC (Fabaceae)

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Aimé Cézaire Ayéna ◽  
Kokou Anani ◽  
Kossi Dosseh ◽  
Amegnona Agbonon ◽  
Messanvi Gbeassor
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Leaf Extracts ◽  
E Coli ◽  
Broth Microdilution Method ◽  
Anti Inflammatory ◽  
Pterocarpus Santalinoides

Aims. Pterocarpus santalinoides is used in Beninese folk medicine for treatment of gastroenteritis. This study aims to compare the antimicrobial, antioxidant, and anti-inflammatory activity of the hydroalcoholic extracts of the leaves, trunk bark, and root. Materials and Methods. The antimicrobial activity was evaluated by the broth microdilution method on 06 bacterial strains including 03 wild-type strains (Escherichia. coli 0157H, Salmonella sp., and Staphylococcus aureus sp.) and 03 reference strains (E. coli ATCC 25922, S. aureus ATCC 29213, and Pseudomonas aeruginosa ATCC 27853), whereas the anti-inflammatory activity was performed by the carrageenan-induced paw edema method on rats. The DPPH-free radical scavenging was used to determine the antioxidant activity. Results. The MICs of the leaf extracts varied from 6.25 to 25 mg/mL for all strains. The MICs of the stem bark extracts were 6.5 to 25 μg/mL for five strains (E. coli 0157H, S. aureus ATCC 25922, Salmonella sp., E. coli ATCC 25922, and P. aeruginosa ATCC 27853) and 3.125 mg/mL for S. aureus. Concerning the root extracts, the MICs varied from 12.5 to 50 mg/mL. The best anti-inflammatory power was obtained with the stem bark extract with the percentages of inhibition of 36.09%, 38.98%, and 39.50%. The DPPH test showed that the hydroethanolic extract of the 03 parts of P. santalinoides has a moderate antiradical power compared to the control which was quercetin. Conclusion. In view of the different pharmacological activity recorded, the extract of the leaves should be recommended to treat patients suffering from gastroenteriditis.

scholarly journals Antinociceptive and Anti-Inflammatory Activities of Ethanolic Stem Bark Extract of Smilax zeylanica Linn. in Wistar Rats

2021 ◽  
pp. 103-113
Author(s):  
Rasheed Ahemad Shaik ◽  
Fazil Ahmad ◽  
Mohammed Abdul Aleem
Keyword(s):  
Acetic Acid ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Drug Group ◽  
Bark Extract ◽  
Hot Plate ◽  
Paw Oedema ◽  
Stem Bark Extract ◽  
Anti Inflammatory

Background: Smilax zeylanica Linn (Smilaceae) is a woody climbing shrub native to Asia's tropical and subtropical highlands. Traditionally, this plant has been widely used to cure a variety of ailments. Smilax zeylanica stem bark was extracted with ethanol to test its antinociceptive and anti-inflammatory properties. Objective: To study the antinociceptive and anti-inflammatory activities of ethanolic stem bark extract of Smilax zeylanica Linn. in Wistar rats. Methods: The antinociceptive activity has been assessed in mice by formalin-induced paw licking, Eddy’s hot-plate, and acetic-acid induced abdominal stretching models. Carrageenan-induced paw oedema in rats has been conducted to explore the anti-inflammatory activity. Tween 80 (10ml/kg, p.o) was given to the control group, test drug group received ethanol extract of Smilax zeylanica (EESZ) orally at doses of 200 and 400 mg/kg, and standard drugs morphine (5 mg/kg, s.c), diclofenac sodium (10 mg/kg, i.p) were injected to the standard drug group. Results: Oral administration of ethanolic extract of Smilax zeylanica (EESZ) at test doses (200 and 400 mg/kg), produced a significant decrease of the paw licking time for 2 phases (neurogenic and inflammatory) in the formalin test, upsurge of the reaction time in Eddy’s hot plate test, and significantly decreased the abdominal stretching in the acetic acid-induced  writhes test. Ethanol extract of Smilax zeylanica significantly decreased the carrageenan-induced paw oedema. Conclusion: The ethanol extract of Smilax zeylanica possesses antinociceptive, anti-inflammatory activity, probably involving central and peripheral pathways

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