scholarly journals DEVELOPMENT AND VALIDATION OF SPECTROSCOPIC METHODS FOR SIMULTANEOUS ESTIMATION AND DISSOLUTION OF OFLOXACIN AND ORNIDAZOLE IN TABLET DOSAGE FORMS

2018 ◽  
Vol 35 (3) ◽  
pp. 123
Author(s):  
R. C. Mashru ◽  
S V. Saikumar
Keyword(s):  
Dosage Forms ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Spectroscopic Methods ◽  
Dissolution Studies ◽  
Significant Difference ◽  
Development And Validation ◽  
Tablet Dosage ◽  
Regulatory Filing ◽  
Paddle Apparatus

The aim of this work was to develop and validate simple, accurate and precise spectroscopic methods (multicomponent, dual wavelength and simultaneous equations) for the simultaneous estimation and dissolution testing of ofloxacin and ornidazole tablet dosage forms. The medium of dissolution used was 900 ml of 0.01N HCl, using a paddle apparatus at a stirring rate of 50 rpm. The drug release was evaluated by developed and validated spectroscopic methods. Ofloxacin and ornidazole showed 293.4 and 319.6nm as λ max in 0.01N HCl. The methods were validated to meet requirements for a global regulatory filing. The validation included linearity, precision and accuracy. In addition, recovery studies and dissolution studies of three different tablets were compared and the results obtained show no significant difference among products.

scholarly journals Development and validation of UV spectroscopic methods for simultaneous estimation of ciprofloxacin and tinidazole in tablet formulation

2012 ◽  
Vol 1 (10) ◽  
pp. 317-321 ◽  
Author(s):  
Sowjanya Gummadi ◽  
Devi Thota ◽  
Sri Valli Varri ◽  
Pratyusha Vaddi ◽  
Venkata Lakshmi Narasimha Seshagiri Rao
Keyword(s):  
Phosphate Buffer ◽  
Dosage Form ◽  
Pharmaceutical Journal ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Spectroscopic Methods ◽  
Good Reproducibility ◽  
Statistical Validation ◽  
Development And Validation ◽  
Tablet Dosage

Two simple, accurate, precise, reproducible and economical UV spectroscopic methods (A &  B) for simultaneous estimation of Ciprofloxacin and Tinidazole in tablet dosage form have been developed. Method A employs solving of simultaneous equations based on the measurement of absorbance at two wavelengths, 271nm and 318nm which are the ?max values of Ciprofloxacin and Tinidazole respectively in phosphate buffer (pH 6.8). Method B is based on the principle of  Q-Analysis where in the absorbance was measured at 292nm (iso-absorptive point) and 271nm (?max of Ciprofloxacin)in phosphate buffer (pH 6.8). Ciprofloxacin and Tinidazole  shows linearity at all the selected wave-lengths and  obeys Beer’s law in the concentration range of 10-35µg/mL and 10-80µg/mL respectively.  Recovery studies for Ciprofloxacin and Tinidazole were performed and the percentage recovery for both the drugs was obtained in the range of 98.1-99.7% (Method A) and 98.0-100.4% (Method B) confirming the accuracy of the proposed method. Both the methods showed good reproducibility and recovery with %RSD less than 2. Statistical validation of the data shows that the proposed methods can be successfully applied for the routine analysis of drugs in commercial tablets.DOI: http://dx.doi.org/10.3329/icpj.v1i10.11849 International Current Pharmaceutical Journal 2012, 1(10): 317-321 

scholarly journals DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHODS FOR SIMULTANEOUS ESTIMATION OF SALBUTAMOL SULPHATE AND DOXOPHYLLINE IN COMBINED SOLID DOSAGE FORM

2017 ◽  
Vol 9 (6) ◽  
pp. 117
Author(s):  
Anjoo Kamboj ◽  
Pawan Sidana ◽  
Upendra K. Jain
Keyword(s):  
Phosphate Buffer ◽  
Dosage Form ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Spectroscopic Methods ◽  
Statistical Validation ◽  
Salbutamol Sulphate ◽  
Solid Dosage ◽  
Development And Validation ◽  
Tablet Dosage

Objective: Salbutamol sulphate (SBS) and doxophylline (DOX) was used for the treatment of asthma and bronchitis. In the present study, two simple, accurate, precise, reproducible and economical UV-spectroscopic methods (A and B) for simultaneous estimation of SBS and DOX in tablet dosage form have been developed.Methods: In the present study the simultaneous estimation of SBS and DOX was carried out by two methods. Method A employs solving of simultaneous equations based on the measurement of absorbance at two wavelengths, 272 nm and 276 nm which are the 𝜆max values of SBS and DOX respectively in phosphate buffer (pH 7.4). Method B is based on the principle of Q-Analysis where in, absorbance was measured at 225 nm (iso-absorptive point, 𝜆1) and 276 nm (𝜆max of DOX, 𝜆2) in phosphate buffer (pH 7.4).Results: Both SBS and DOX shows linearity at all the selected wavelengths and obeys beer’s law in the concentration range of between 0.2-1.6𝜇g/ml and 0.1-3.5μg/ml at 276 nm; 0.2-1.6 µg/ml and 0.1-4.5 µg/ml at 272 nm and 0.2-2.0µg/ml and 0.2-3.5μg/ml at iso-absorptive point 225 nm. Recovery studies for SBS and DOX were performed and the percentage recovery for both the drugs was obtained in the range of 97.45-98.63% (Method A) and 97.49–98.87 % (Method B) confirming the accuracy of the proposed method.Conclusion: Both the methods showed good reproducibility and recovery with % RSD less than 2. Statistical validation of the data shows that the proposed methods can be successfully applied for the routine analysis of drugs in commercial tablets. Hence, it could be used in the analysis of laboratory samples and marketed formulations containing these two drugs in combined dosage form without the interference of common excipients.

scholarly journals Development and validation of analytical methods for the simultaneous estimation of Nimorazole and Ofloxacin in tablet dosage form

2016 ◽  
Vol 8 (3) ◽  
pp. 96
Author(s):  
A P Ghugare ◽  
Lalchand Dayanand Devhare ◽  
B P Hatwar
Keyword(s):  
Analytical Methods ◽  
Dosage Form ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Distilled Water ◽  
Spectrophotometric Methods ◽  
Ratio Equation ◽  
Absorbance Ratio ◽  
Development And Validation ◽  
Tablet Dosage

<p>Two simple, rapid, accurate and precise spectrophotometric methods have been developed for simultaneous estimation of Nimorazole and Ofloxacin from tablet dosage form. Method І involves formation of ‘simultaneous equations’ at 304 nm (λ max of Nimorazole) and 287.5 nm (λ max of Ofloxacin); while Method ІІ involves, formation of ‘Absorbance ratio equation’ at 301(isoabsorptive point) and 287.5 nm (λ max of Ofloxacin) using distilled water as a solvent. The linearity was observed in the concentration range of 5 - 25 μg/ml for Nimorazole and 2 - 10 μg/ml for Ofloxacin. The results of analysis have been validated statistically and by recovery studies and were found satisfactory.</p>

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF PROCHLORPERAZINE MALEATE AND PYRIDOXINE HYDROCHLORIDE IN TABLET DOSAGE FORM BY UV USING MULTI-COMPONENT MODE OF ANALYSIS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (03) ◽  
pp. 20-25
Author(s):  
G. B Bhagwat ◽  
◽  
S. P Wate ◽  
A. S. Mundhey
Keyword(s):  
Efficient Method ◽  
Dosage Form ◽  
Uv Spectroscopy ◽  
Dosage Forms ◽  
Simultaneous Estimation ◽  
Analysis Method ◽  
Pyridoxine Hydrochloride ◽  
Development And Validation ◽  
Tablet Dosage

Prochlorperazine maleate and pyridoxine hydrochloride in combination are available as tablet dosage forms in the ratio of 1:5. A simple, reproducible and efficient method for the simultaneous determination of prochlorperazine maleate and pyridoxine hydrochloride in tablet dosage form has been developed. The developed method is based on the simultaneous estimation by UV Spectroscopy, using multi-component mode of analysis. In this method 0.3M HCl was used as solvent. Wavelengths selected for estimation of prochlorperazine maleate and pyridoxine hydrochloride in multi-component mode of analysis method were 254.5 nm and 290.5 nm respectively. Both drugs obey Beer-Lambert’s law in concentration range of 1-5 µg/mL (prochlorperazine maleate) and 5-25 µg/mL (pyridoxine hydrochloride). The results of analysis have been validated statistically and by recovery studies.

Sign in / Sign up

Export Citation Format

Share Document