DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF PROCHLORPERAZINE MALEATE AND PYRIDOXINE HYDROCHLORIDE IN TABLET DOSAGE FORM BY UV USING MULTI-COMPONENT MODE OF ANALYSIS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (03) ◽  
pp. 20-25
Author(s):  
G. B Bhagwat ◽  
◽  
S. P Wate ◽  
A. S. Mundhey
Keyword(s):  
Efficient Method ◽  
Dosage Form ◽  
Uv Spectroscopy ◽  
Dosage Forms ◽  
Simultaneous Estimation ◽  
Analysis Method ◽  
Pyridoxine Hydrochloride ◽  
Development And Validation ◽  
Tablet Dosage

Prochlorperazine maleate and pyridoxine hydrochloride in combination are available as tablet dosage forms in the ratio of 1:5. A simple, reproducible and efficient method for the simultaneous determination of prochlorperazine maleate and pyridoxine hydrochloride in tablet dosage form has been developed. The developed method is based on the simultaneous estimation by UV Spectroscopy, using multi-component mode of analysis. In this method 0.3M HCl was used as solvent. Wavelengths selected for estimation of prochlorperazine maleate and pyridoxine hydrochloride in multi-component mode of analysis method were 254.5 nm and 290.5 nm respectively. Both drugs obey Beer-Lambert’s law in concentration range of 1-5 µg/mL (prochlorperazine maleate) and 5-25 µg/mL (pyridoxine hydrochloride). The results of analysis have been validated statistically and by recovery studies.

scholarly journals PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI (KCKT) UNTUK ESTIMASI KADAR SIMULTAN ANTIEMETIK PIRIDOKSIN HIDROKLORIDA DAN PIRATIAZIN TEOKLAT DALAM BENTUK SEDIAAN TABLET

2019 ◽  
Vol 6 (2) ◽  
pp. 95
Author(s):  
Fikri Alatas ◽  
Hernandi Sujono ◽  
Woro Artati Sucipto
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Dosage Form ◽  
Ultra Violet ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Pyridoxine Hydrochloride ◽  
Development And Validation ◽  
Tablet Dosage

<p align="center"><strong>Abstrak</strong><strong></strong></p><p align="center"><strong> </strong></p><p>Metode kromatografi cair kinerja tinggi (KCKT) dengan detektor ultra lembayung telah dikembangkan dan divalidasi untuk estimasi kadar secara simultan campuran piridoksin hidroklorida (PH) dan piratiazin teoklat (PT)dalam sediaan tablet antiemetik. Proses pemisahan terjadi dalam kolom Inertsil® ODS-3 pada panjang gelombang 280 nm dengan laju alir 1,0 mL/menit. Fase gerak yang optimal untuk pemisahan adalah campuran methanol-asam asetat 1% (20:80) dengan waktu retensi PH dan PT berturut-turut adalah 1,2 dan 9,8 menit. Perolehan kembali PH dan PT berturut-turut adalah 100,13 dan 99,78 %. Batas deteksi untuk PH dan PT berturut-turut adalah 0,21 dan 0,22 µg/mL, sedangkan batas kuantisasinya berturut-turut adalah 0,70 dan  0,72 µg/mL. Metode ini dapat diterapkan sebagai metode untuk estimasi kadar campuran piridoksin hidroklorida dan piratiazin teoklat dalam bentuk sediaan tablet secara simultan.</p><p> </p><p><strong>Kata kunci:</strong> Piridoksin hidroklorida, piratiazin teoklat, KCKT, tablet</p><p> </p><p align="center"><strong><em>Development and validation of high performance liquid chromatography (HPLC) method for simultaneous estimation of antiemetic pyridoxyne hydrochloride and pyrathiazine theoclate in tablet dosage form</em></strong></p><p> </p><p align="center"><strong><em>Abstract</em></strong><strong><em></em></strong></p><p><em> </em></p><p><em>The high performance liquid chromatography (HPLC) method with an ultra violet detector has been developed and validated for simultaneous estimation of pyridoxine hydrochloride (PH) and pyrathiazine theoclate (PT) in antiemetic tablet preparations. The separation process occurs in the Inertsil® ODS-3 column at a wavelength of 280 nm with a flow rate of 1.0 mL /min. The optimal mobile phase for separation is a mixture of methanol-acetic acid 1% (20:80) with the retention times of PH and PT 1.2 and 9.8 minutes respectively. The recoveries of PH and PT were 100.13 and 99.78%, respectively. The detection limits for PH and PT were 0.21 and 0.22 µg / mL respectively, while the quantisation limits were 0.70 and 0.72 µg / mL, respectively. This method can be applied as a method for simultaneous estimating the levels of pyridoxine hydrochloride and pyrathiazine theoclate in tablet dosage form.</em><em></em></p><p><em> </em></p><p><strong><em>Keywords:</em></strong><em> Pyridoxine hydrochloride, pyrathiazine theoclate, HPLC, tablet</em></p>

scholarly journals Development and validation of stability-indicating RP-UPLC method for simultaneous estimation of thiocolchicoside and aceclofenac in combined dosage form

2014 ◽  
Vol 3 (7) ◽  
pp. 296-300 ◽  
Author(s):  
Paramasivam Balan ◽  
Nagappan Kannappan
Keyword(s):  
Dosage Form ◽  
Pharmaceutical Journal ◽  
Detector Response ◽  
Simultaneous Estimation ◽  
Forced Degradation ◽  
Stability Indicating ◽  
The Stability ◽  
Development And Validation ◽  
Tablet Dosage

A stability indicating RP-UPLC method was developed and validated for the simultaneous determination of Thiocolchicoside (TCC) and Aceclofenac (ACF) in tablet dosage form. The chromatographic separation was carried out by Thermo Scientific UPLC Instrument, Accela 1250 Pump, auto sampler with PDA detector, using column Thermo Scientific hypersil gold C18, (50 x 2.1mm) particle size 1.9µm using 5% ammonium acetate buffer and methanol in the ratio of 40:60, pH was adjusted to 5 with ortho phosphoric acid as mobile phase at a flow rate of 250 µl/min with the detection at 276nm. The run times of the TCC and ACF were about 0.697 and 1.125 minutes, respectively. The detector response is linear from 4.8 µg/ml to 7.2 µg/ml and 63.8 µg/ml to 96 µg/ml concentrations for TCC and ACF respectively. The linear regression equation was found to be y = 20620x-677.68 (r2 = 0.9996) for TCC and y= 50931x-319.3 (r2 = 0.9997) for ACF. The detection limit and quantification limit was 0.076µg and 0.23µg for TCC and 0.27µg and 0.71µg for ACF. The percentage of assay of TCC and ACF were about 99.50% and 99.96% respectively. The stability indicating capability was established by forced degradation experiments. The method was satisfactorily validated as per the ICH guidelines.DOI: http://dx.doi.org/10.3329/icpj.v3i7.19078 International Current Pharmaceutical Journal, June 2014, 3(7): 296-300

DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF FAMOTIDINE, DICLOFENAC AND PARACETAMOL IN THEIR COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (09) ◽  
pp. 60-64
Author(s):  
P. P Desai ◽  
◽  
N. R. Patel ◽  
H. G Bhatt
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Simultaneous Estimation ◽  
Absorbance Measurement ◽  
Validation Parameters ◽  
Stock Solutions ◽  
Development And Validation ◽  
Tablet Dosage

Famotidine (FAM), Diclofenac (DCF) and Paracetamol (PCM) are used in combination for musculoskeletal disorders. A simple, sensitive, rapid, precise, reproducible and accurate spectrophotometric method for simultaneous determination of FAM, DCF and PCM was developed. The method was based on UV spectrophotometric determination of three drugs using simultaneous equation method. The stock solutions were prepared in methanol AR. Absorbance measurement was carried out at 288.4 nm, 281.2nm and 248.2 nm for FAM, DCF and PCM respectively. Beer Lambert law was obeyed in the concentration range of 1-30μg/mL for FAM, 2-40μg/mL for DCF and 1-20μg/mL for PCM. The results of the analysis were tested and validated for various validation parameters statistically and by recovery studies according to the International Conference on Harmonization Q2B guidelines. The utility of the developed method has been demonstrated by analysis of commercially available tablet dosage form.

scholarly journals Development and Validation of UV-Spectrophotometric Method for Estimation of Metformin Hydrochloride and Pioglitazone in Tablet Dosage Form

2019 ◽  
Vol 9 (4-A) ◽  
pp. 381-384
Author(s):  
Rupali S. Joshi ◽  
Ajit K. Nangare ◽  
Deepali S. Sanap ◽  
Surekha M. Sase
Keyword(s):  
Dosage Form ◽  
Estimation Method ◽  
Pharmaceutical Formulations ◽  
Dosage Forms ◽  
Dual Wavelength ◽  
Metformin Hydrochloride ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Development And Validation ◽  
Tablet Dosage

Simple, sensitive, rapid and accurate UV spectroscopic methods have been developed for the estimation of metformin hydrochloride and pioglitazone in tablet dosage forms. Simultaneous estimation and dual-wavelength methods were developed and validated using solvent methanol. Both drugs show linearity at 5-40 µg/ml for both methods. The suggested techniques have been effectively implemented in pharmaceutical formulations to the evaluation of quoted drugs. Recovery research was conducted to verify the method's accuracy, precision. The techniques have been validated under ICH guidelines. Keywords: Metformin hydrochloride, Pioglitazone, Simultaneous estimation method and Dual wavelength method.

scholarly journals Development and Validation of Absorption Correction UV-Spectrophotometry Method for Simultaneous Estimation of Ranitidine HCL and Dicyclomine Hydrochloride in Their Marketed Formulation

2019 ◽  
pp. 851-863
Author(s):  
Bhumi Kantariya ◽  
Greeva Bhatt ◽  
Mehul Mehta ◽  
Urmi Kanatria ◽  
Ravi Dalsaniya
Keyword(s):  
Dosage Form ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Rapid Absorption ◽  
Absorption Correction ◽  
Naoh Solution ◽  
Development And Validation ◽  
Tablet Dosage ◽  
Ich Guideline

A Simple, selective, precise and rapid absorption correction Spectrophotometric method has been developed and validated as per ICH guideline for the simultaneous estimation of Ranitidine Hydrochloride and Dicyclomine Hydrochloride in tablet dosage form. Method is base on UV Spectrophotometric for determination of two drug, by using Methanol as a solvent and diluted the same with 0.1N NaOH, solution. In this UV method, the two wavelength were selected, 311.4 nm and at 217 nm for RANTD and DICY, respectively. this method was validated according to ICH guideline and Linearity range, was found to be 7.5-37.5 µg/ml and 1-5 µg/ml for RANTD and DICY, respectively. The method was successfully applied to assay drugs in tablet.

scholarly journals Development and validation of new analytical method for the simultaneous estimation of omeprazole and domperidone in pharmaceutical dosage form by UV spectrophotometry

2019 ◽  
Vol 1 (2) ◽  
pp. 44-46
Author(s):  
V. Pavan Kumar ◽  
C. Bhanu Chandra ◽  
N. Devendra ◽  
M. Kishor Kumar ◽  
S. Reddy Basha ◽  
...  
Keyword(s):  
Dosage Form ◽  
Simultaneous Equation ◽  
Dosage Forms ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Pharmaceutical Dosage Form ◽  
Pharmaceutical Dosage Forms ◽  
Ich Guidelines ◽  
Development And Validation

A simple, rapid and precise method was developed for the quantitative simultaneous determination of Omeprazole and Domperidone in combined pharmaceutical-dosage forms. The method was based on UV-Spectrophotometric determination of two drugs, using simultaneous equation method. It involves absorbance measurement at 291 nm (λmax of Omeprazole) and 289 nm (λmax of Domperidone) in Methanol: Acetonitrile (30:70 v/v). For UV Spectrophotometric method, linearity was obtained in concentration range of 1-15 µg/ml for Domperidone and 1-50 µg/ml for Omeprazole respectively, with regression 0.999 and 0.999 for Domperidone and Omeprazole respectively. Recovery was in the range of 99 -103%; the value of standard deviation and %R.S.D were found to be < 2 %; shows the high precision of the method., in accordance with ICH guidelines. The method has been successively applied to pharmaceutical formulation and was validated according to ICH guidelines.

scholarly journals Simultaneous UV Spectrophotometric Determination of Cetrizine and Dextromethorphan in Tablet Dosage Form

2010 ◽  
Vol 7 (s1) ◽  
pp. S314-S318 ◽  
Author(s):  
R. Vijayalakshmi ◽  
S. Bhargavi ◽  
M. D. Dhanaraju
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Determination ◽  
Dosage Form ◽  
Dosage Forms ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Isobestic Point ◽  
Ich Guidelines ◽  
Tablet Dosage

Two accurate, precise, sensitve and economical procedures for simultaneous estimation of cetrizine and dextromethorphan in tablet dosage forms have been developed. First method employs formation and solving of simultaneous equations using 230 nm and 280 nm as two analytical wavelengths for both drugs in methanol. The second method isQ-analysis based on measurement of absorptivity at 224 nm (as isobestic point) and 280 nm (λmaxof CTZ). Cetrizine and dextromethorphan at their respective λmax280 nm and 230 nm and at 224 nm (isobestic point) shows linearity in a concentration range of 10-30 mcg/mL for both the drugs. The recovery studies confirmed accuracy of the proposed methods and low values of standard deviation confirmed precision of the methods. The methods were validated as per ICH guidelines.

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