Dental Pulp Vitality and Dentin Deposition in Injured, Immature Teeth of Dogs

Dental pulp testing is a useful and essential diagnostic aid in endodontics. Pulp sensibility tests include thermal and electric tests, which extrapolate pulp health from sensory response. Whilst pulp sensibility tests are the most commonly used in clinical practice, they are not without limitations and shortcomings. Pulp vitality tests attempt to examine the presence of pulp blood flow, as this is viewed as a better measure of true health than sensibility. Laser Doppler flowmetry and pulse oximetry are examples of vitality tests. Whilst the prospect is promising, there are still many practical issues that need to be addressed before vitality tests can replace sensibility tests as the standard clinical pulp diagnostic test. With all pulp tests, the results need to be carefully interpreted and closely scrutinised as false results can lead to misdiagnosis which can then lead to incorrect, inappropriate, or unnecessary treatment.

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Regeneration of dental pulp tissue in immature teeth with apical periodontitis using platelet-rich plasma and dental pulp cells

International Endodontic Journal ◽

10.1111/iej.12087 ◽

2013 ◽

Vol 46 (10) ◽

pp. 962-970 ◽

Cited By ~ 61

Author(s):

W. Zhu ◽

X. Zhu ◽

G. T.-J. Huang ◽

G. S. P. Cheung ◽

W. L. Dissanayaka ◽

...

Keyword(s):

Dental Pulp ◽

Platelet Rich Plasma ◽

Apical Periodontitis ◽

Dental Pulp Cells ◽

Pulp Tissue ◽

Immature Teeth ◽

Dental Pulp Tissue ◽

Pulp Cells

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Verification of accuracy of an algorithmic image-based dental pulp vitality test

Medical Imaging 2020: Computer-Aided Diagnosis ◽

10.1117/12.2549744 ◽

2020 ◽

Author(s):

Sarah Bi ◽

Laura Martinez ◽

Justin Bequette ◽

Andrew Peitzsch ◽

William D'Angelo

Keyword(s):

Dental Pulp ◽

Vitality Test ◽

Pulp Vitality

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Expression of early angiogenesis indicators in mature versus immature teeth

BMC Oral Health ◽

10.1186/s12903-020-01313-1 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Javier Caviedes-Bucheli ◽

Luis F. Lopez-Moncayo ◽

Hernan Dario Muñoz-Alvear ◽

Francisco Hernandez-Acosta ◽

Melissa Pantoja-Mora ◽

...

Keyword(s):

Root Development ◽

Dental Pulp ◽

Cell Metabolism ◽

Elisa Test ◽

Physiological Process ◽

Oxygen Balance ◽

Third Molars ◽

Angiogenic Activity ◽

Immature Teeth ◽

Development Group

Abstract Background Proper oxygen balance in the dental pulp is essential for cell metabolism. Angiogenesis in the pulp is a constant process during the life of the tooth. Hypoxia indicators in a tissue, such as HIF-1α, as well as vascular destabilization markers, such as ANG2 and its receptor TIE2, are necessary for angiogenesis. Therefore the purpose of this study is to evaluate the expression of HIF-1α, ANG1, ANG2 and TIE2 in dental pulp as early angiogenesis indicators in teeth with complete and incomplete root development. Methods Forty human dental pulps were obtained from freshly extracted third molars divided into two groups: incomplete (n = 20) and complete (n = 20) root development. Dental pulps were stored at − 80 °C, defrosted in an ice bath and re-frozen with their respective thaws to disintegrate the tissue. Three sonication cycles were performed until the tissues were homogenized, then thaw were centrifuged and the supernatant was collected for the detection of the markers to be studied. The samples were processed for the ELISA test using the ELISA-sandwich principle. Student t and Mann–Whitney U tests were performed to determine statistically significant differences between groups. Results In the complete root development, HIF-1α, ANG1, ANG2 and TIE2 expressions were significantly higher than their expression in the incomplete root development group. Conclusions The angiogenic process seems to be a physiological process in the dental pulp. Angiogenic activity is higher in teeth with mature than immature apex teeth.

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