scholarly journals Spectroscopic Study of Human Teeth and Blood from Visible to Terahertz Frequencies for Clinical Diagnosis of Dental Pulp Vitality

2012 ◽  
Vol 33 (3) ◽  
pp. 366-375 ◽  
Author(s):  
Marion Hirmer ◽  
Sergey N. Danilov ◽  
Stephan Giglberger ◽  
Jürgen Putzger ◽  
Andreas Niklas ◽  
...  
Keyword(s):  
Spectroscopic Study ◽  
Clinical Diagnosis ◽  
Dental Pulp ◽  
Human Teeth ◽  
Terahertz Frequencies ◽  
Pulp Vitality

scholarly journals Dental Pulp-Derived Mesenchymal Stem Cells for Modeling Genetic Disorders

2021 ◽  
Vol 22 (5) ◽  
pp. 2269
Author(s):  
Keiji Masuda ◽  
Xu Han ◽  
Hiroki Kato ◽  
Hiroshi Sato ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dental Pulp ◽  
Genetic Disorders ◽  
In Vitro Models ◽  
High Plasticity ◽  
Human Teeth ◽  
Clinical Diagnoses ◽  
Models Of Disease

A subpopulation of mesenchymal stem cells, developmentally derived from multipotent neural crest cells that form multiple facial tissues, resides within the dental pulp of human teeth. These stem cells show high proliferative capacity in vitro and are multipotent, including adipogenic, myogenic, osteogenic, chondrogenic, and neurogenic potential. Teeth containing viable cells are harvested via minimally invasive procedures, based on various clinical diagnoses, but then usually discarded as medical waste, indicating the relatively low ethical considerations to reuse these cells for medical applications. Previous studies have demonstrated that stem cells derived from healthy subjects are an excellent source for cell-based medicine, tissue regeneration, and bioengineering. Furthermore, stem cells donated by patients affected by genetic disorders can serve as in vitro models of disease-specific genetic variants, indicating additional applications of these stem cells with high plasticity. This review discusses the benefits, limitations, and perspectives of patient-derived dental pulp stem cells as alternatives that may complement other excellent, yet incomplete stem cell models, such as induced pluripotent stem cells, together with our recent data.

scholarly journals Dental Pulp Testing: A Review

2009 ◽  
Vol 2009 ◽  
pp. 1-12 ◽  
Author(s):  
Eugene Chen ◽  
Paul V. Abbott
Keyword(s):  
Blood Flow ◽  
Clinical Practice ◽  
Pulse Oximetry ◽  
Diagnostic Test ◽  
Laser Doppler Flowmetry ◽  
Dental Pulp ◽  
Sensory Response ◽  
Diagnostic Aid ◽  
Doppler Flowmetry ◽  
Pulp Vitality

Dental pulp testing is a useful and essential diagnostic aid in endodontics. Pulp sensibility tests include thermal and electric tests, which extrapolate pulp health from sensory response. Whilst pulp sensibility tests are the most commonly used in clinical practice, they are not without limitations and shortcomings. Pulp vitality tests attempt to examine the presence of pulp blood flow, as this is viewed as a better measure of true health than sensibility. Laser Doppler flowmetry and pulse oximetry are examples of vitality tests. Whilst the prospect is promising, there are still many practical issues that need to be addressed before vitality tests can replace sensibility tests as the standard clinical pulp diagnostic test. With all pulp tests, the results need to be carefully interpreted and closely scrutinised as false results can lead to misdiagnosis which can then lead to incorrect, inappropriate, or unnecessary treatment.

scholarly journals Evaluation of secondary dentin formation for forensic age assessment by means of semi-automatic segmented ultrahigh field 9.4 T UTE MRI datasets

2020 ◽  
Vol 134 (6) ◽  
pp. 2283-2288
Author(s):  
Maximilian Timme ◽  
Jens Borkert ◽  
Nina Nagelmann ◽  
Andreas Schmeling
Keyword(s):  
Dental Pulp ◽  
Automatic Segmentation ◽  
Tooth Pulp ◽  
Human Teeth ◽  
Age Assessment ◽  
Ultrahigh Field ◽  
Tooth Structure ◽  
Pulp Cavity ◽  
Magnetic Resonance Imaging Mri ◽  
Dentin Formation

Abstract Dental methods are an important element of forensic age assessment of living persons. After the development of all the teeth, including third molars, is completed, degenerative characteristics can be used to assess age. The radiologically detectable reduction of the dental pulp cavity has been described as such a feature. We investigated the suitability of ultrahigh field 9.4 T ultrashort time echo (UTE) magnetic resonance imaging (MRI) for the evaluation of pulp cavity volume in relation to the total tooth volume in 4 extracted human teeth. The volume calculations were performed after semi-automatic segmentation by software AMIRA using the different intensities of the structures in the MRI dataset. The automatically selected intensity range was adjusted manually to the structures. The visual distinction of pulp and tooth structure was possible in all cases with in-plane resolution < 70 μm. Ratios of tooth/pulp volume were calculated, which could be suitable for age estimation procedures. Intensity shifts within the pulp were not always correctly assigned by the software in the course of segmentation. 9.4 T UTE-MRI technology is a forward-looking, radiation-free procedure that allows the volume of the dental pulp to be determined at high spatial resolution and is thus potentially a valuable instrument for the age assessment of living persons.

scholarly journals Dental pulp stem cells from human teeth with deep caries displayed an enhanced angiogenesis potential in vitro

2021 ◽  
Vol 16 (1) ◽  
pp. 318-326
Author(s):  
Yan Chen ◽  
Xinzhu Li ◽  
Jingyi Wu ◽  
Wanyu Lu ◽  
Wenan Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Teeth

Verification of accuracy of an algorithmic image-based dental pulp vitality test

2020 ◽  
Author(s):  
Sarah Bi ◽  
Laura Martinez ◽  
Justin Bequette ◽  
Andrew Peitzsch ◽  
William D'Angelo
Keyword(s):  
Dental Pulp ◽  
Vitality Test ◽  
Pulp Vitality

Updated review on methods to determine dental pulp vitality

2015 ◽  
Vol 67 (6) ◽  
pp. 872-886
Author(s):  
Olga Rudzka ◽  
Rodrigo Giacaman ◽  
Renata Chałas
Keyword(s):  
Dental Pulp ◽  
Pulp Vitality

Russell bodies in dental pulp of permanent human teeth

2004 ◽  
Vol 98 (6) ◽  
pp. 760-764 ◽  
Author(s):  
Luciano Giardino ◽  
Enrico Savoldi ◽  
Francesco Pontieri ◽  
Elio Berutti
Keyword(s):  
Dental Pulp ◽  
Human Teeth

scholarly journals Isolation and culture of dental pulp stem cells from permanent and deciduous teeth

2019 ◽  
Vol 35 (4) ◽  
Author(s):  
Shagufta Naz ◽  
Farhan Raza Khan ◽  
Raheela Rahmat Zohra ◽  
Sahreena Salim Lakhundi ◽  
Mehwish Sagheer Khan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Tooth Pulp ◽  
Human Teeth ◽  
Creative Commons ◽  
Calcified Tissue

Objective: To isolate dental pulp mesenchymal stem cells (MSCs) from non-infected human permanent and deciduous teeth. Methods: It was an in-vitro experimental study. Human teeth were collected from 13 apparently healthy subjects including nine adults and four children. After decoronation dental pulps were extirpated from teeth and cultured via explant method in a stem cell defined media. Data was analyzed by descriptive statistics. Results: As above MSCs emerged exhibiting fibroblast-like morphology. In vitro culture was positive for 100% (9/9) and 75% (3/4) of the permanent and deciduous teeth respectively. First cell appeared from deciduous teeth pulp in 10±6.2 days while permanent teeth pulp took 12.4±3.7 days. Together, 26.6±3.6 and 24.5±3.5 days were required for permanent and deciduous tooth pulp stem cells to be ready for further assays. Conclusions: The protocol we developed is easy and consistent and can be used to generate reliable source of MScs for engineering of calcified and non-calcified tissue for regenerative medicine approaches. doi: https://doi.org/10.12669/pjms.35.4.540 How to cite this:Naz S, Khan FR, Zohra RR, Lakhundi SS, Khan MS, Mohammed N, et al. Isolation and culture of dental pulp stem cells from permanent and deciduous teeth. Pak J Med Sci. 2019;35(4):---------. doi: https://doi.org/10.12669/pjms.35.4.540 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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