scholarly journals Red Blood Cell Indices for Rainbow Trout (Oncorhynchus mykiss) Reared in Cage and Raceway Culture

2004 ◽  
Vol 73 (1) ◽  
pp. 105-114 ◽  
Author(s):  
J. Řehulka ◽  
V. Adamec
Keyword(s):  
Rainbow Trout ◽  
Blood Cell ◽  
Oncorhynchus Mykiss ◽  
Red Blood Cell ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Red Blood Cell Indices

scholarly journals Evaluation of Soybean Meal-Red Blood Cell Coextruded Feed Ingredient in Diets Fed to Rainbow Trout Oncorhynchus mykiss

2001 ◽  
Vol 32 (4) ◽  
pp. 409-415 ◽  
Author(s):  
George L. Selden ◽  
Paul B. Brown ◽  
Anthony C. Ostrowski ◽  
Rolando A. Flores ◽  
Lawrence A. Johnson
Keyword(s):  
Rainbow Trout ◽  
Blood Cell ◽  
Oncorhynchus Mykiss ◽  
Red Blood Cell ◽  
Soybean Meal ◽  
Feed Ingredient ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals Acute and chronic influence of temperature on red blood cell anion exchange

2001 ◽  
Vol 204 (1) ◽  
pp. 39-45 ◽  
Author(s):  
F.B. Jensen ◽  
T. Wang ◽  
J. Brahm
Keyword(s):  
Rainbow Trout ◽  
Blood Cell ◽  
Anion Exchange ◽  
Temperature Sensitivity ◽  
Red Blood Cell ◽  
Temperature Acclimation ◽  
Freshwater Turtle ◽  
Acclimation Temperature ◽  
Published Data ◽  
Rainbow Trout Oncorhynchus Mykiss

Unidirectional (36)Cl(−) efflux via the red blood cell anion exchanger was measured under Cl(−) self-exchange conditions (i.e. no net flow of anions) in rainbow trout Oncorhynchus mykiss and red-eared freshwater turtle Trachemys scripta to examine the effects of acute temperature changes and acclimation temperature on this process. We also evaluated the possible adaptation of anion exchange to different temperature regimes by including our previously published data on other animals. An acute temperature increase caused a significant increase in the rate constant (k) for unidirectional Cl(−) efflux in rainbow trout and freshwater turtle. After 3 weeks of temperature acclimation, 5 degrees C-acclimated rainbow trout showed only marginally higher Cl(−) transport rates than 15 degrees C-acclimated trout when compared at the same temperature. Apparent activation energies for red blood cell Cl(−) exchange in trout and turtle were lower than values reported in endothermic animals. The Q(10) for red blood cell anion exchange was 2.0 in trout and 2.3 in turtle, values close to those for CO(2) excretion, suggesting that, in ectothermic animals, the temperature sensitivity of band-3-mediated anion exchange matches the temperature sensitivity of CO(2) transport (where red blood cell Cl(−)/HCO(3)(−) exchange is a rate-limiting step). In endotherms, such as man and chicken, Q(10) values for red blood cell anion exchange are considerably higher but are no obstacle to CO(2) transport, because body temperature is normally kept constant at values at which anion exchange rates are high. When compared at constant temperature, red blood cell Cl(−) permeability shows large differences among species (trout, carp, eel, cod, turtle, alligator, chicken and man). Cl(−) permeabilities are, however, remarkable similar when compared at preferred body temperatures, suggesting an appropriate evolutionary adaptation of red blood cell anion exchange function to the different thermal niches occupied by animals.

scholarly journals Time course of red blood cell intracellular pH recovery following short-circuiting in relation to venous transit times in rainbow trout, Oncorhynchus mykiss

2018 ◽  
Vol 315 (2) ◽  
pp. R397-R407 ◽  
Author(s):  
Till S. Harter ◽  
Alexandra G. May ◽  
William J. Federspiel ◽  
Claudiu T. Supuran ◽  
Colin J. Brauner
Keyword(s):  
Rainbow Trout ◽  
Blood Cell ◽  
Intracellular Ph ◽  
Red Blood Cell ◽  
Time Course ◽  
Ph Sensitive ◽  
Adrenergic Stimulation ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Athletic Ability

Accumulating evidence is highlighting the importance of a system of enhanced hemoglobin-oxygen (Hb-O2) unloading for cardiovascular O2 transport in teleosts. Adrenergically stimulated sodium-proton exchangers (β-NHE) create H+ gradients across the red blood cell (RBC) membrane that are short-circuited in the presence of plasma-accessible carbonic anhydrase (paCA) at the tissues; the result is a large arterial-venous pH shift that greatly enhances O2 unloading from pH-sensitive Hb. However, RBC intracellular pH (pHi) must recover during venous transit (31–90 s) to enable O2 loading at the gills. The halftimes ( t1/2) and magnitudes of RBC β-adrenergic stimulation, short-circuiting with paCA and recovery of RBC pHi, were assessed in vitro, on rainbow trout whole blood, and using changes in closed-system partial pressure of O2 as a sensitive indicator for changes in RBC pHi. In addition, the recovery rate of RBC pHi was assessed in a continuous-flow apparatus that more closely mimics RBC transit through the circulation. Results indicate that: 1) the t1/2 of β-NHE short-circuiting is likely within the residence time of blood in the capillaries, 2) the t1/2 of RBC pHi recovery is 17 s and within the time of RBC venous transit, and 3) after short-circuiting, RBCs reestablish the initial H+ gradient across the membrane and can potentially undergo repeated cycles of short-circuiting and recovery. Thus, teleosts have evolved a system that greatly enhances O2 unloading from pH-sensitive Hb at the tissues, while protecting O2 loading at the gills; the resulting increase in O2 transport per unit of blood flow may enable the tremendous athletic ability of salmonids.

scholarly journals Effects of heat stress on Hsp90a2b and Hsp10 mRNA expression and blood cell apoptosis in rainbow trout Oncorhynchus mykiss (Walbaum, 1972)

2019 ◽  
Vol 66 (4) ◽  
Author(s):  
Zhicheng Luo ◽  
Yanjing Zhou ◽  
Zhe Liu ◽  
Binpeng Xia ◽  
Yongjie Wang
Keyword(s):  
Heat Stress ◽  
Rainbow Trout ◽  
Heat Shock ◽  
Blood Cell ◽  
Oncorhynchus Mykiss ◽  
Blood Cells ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Shock Response ◽  
Heat Shock Protein Genes ◽  
Increasing Temperature

To study the response to heat stress in rainbow trout Oncorhynchus mykiss (Walbaum, 1972), the expression of heat shock protein genes Hsp90a2b and Hsp10 in selected tissues and apoptosis of blood cells were investigated at water temperatures of 18, 21, 23, 24, 25 and 26°C. Expressions of Hsp90a2b in the gill, mid-kidney, brain, heart and muscle were generally upregulated with increasing temperature. Hsp90a2b expression in the liver increased rapidly at 21°C and then showed a significant decrease and finally a significant increase at 25°C and 26°C (p<0.05). Hsp10 expression showed an irregular but overall increasing tendency in gill, spleen and mid-kidney and a wave-like increasing pattern in liver, heart and muscle. Levels of Hsp90a2b at 25°C and Hsp10 at 26°C in all tissues were always significantly higher compared to 18°C (p<0.05). The apoptosis rate at 21°C was significantly higher than that at 18°C, followed by a fall and was the highest at 25°C. All these data indicated that the heat shock response already occurred at 21°C which induced expressions of Hsp90a2b and Hsp10 as well as blood cells apoptosis and 25°C may be a key temperature for stress response in rainbow trout.

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