scholarly journals Vitis sp. response to Xylella fastidiosa strain CoDiRO

EFSA Journal ◽  
2015 ◽  
Vol 13 (11) ◽  
Author(s):  
Keyword(s):  
Xylella Fastidiosa ◽  
Fastidiosa Strain ◽  
Vitis Sp

scholarly journals Draft Genome Sequence of Xylella fastidiosa subsp. fastidiosa Strain Stag’s Leap

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
J. Chen ◽  
F. Wu ◽  
Z. Zheng ◽  
X. Deng ◽  
L. P. Burbank ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Genome Sequence ◽  
Gene Function ◽  
Xylella Fastidiosa ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Pierce's Disease ◽  
Pierce’S Disease ◽  
Knockout Mutants ◽  
Fastidiosa Strain

Xylella fastidiosa subsp. fastidiosa causes Pierce’s disease of grapevine. Presented here is the draft genome sequence of the Stag’s Leap strain, previously used in pathogenicity/virulence assays to evaluate grapevine germplasm bearing Pierce’s disease resistance and a phenotypic assessment of knockout mutants to determine gene function.

Plasmids from Xylella fastidiosa strains

1992 ◽  
Vol 38 (9) ◽  
pp. 993-995 ◽  
Author(s):  
J. Chen ◽  
C. J. Chang ◽  
R. L. Jarret
Keyword(s):  
Key Words ◽  
Ethidium Bromide ◽  
Restriction Site ◽  
Xylella Fastidiosa ◽  
Wilt Disease ◽  
Restriction Map ◽  
Mulberry Leaf ◽  
Fastidiosa Strain ◽  
Ethidium Bromide Staining ◽  
High Degree

Plasmids were observed from three strains of Xylella fastidiosa. A restriction map of a plasmid, pXFPW1 of 1.3 kb, isolated from Xyl. fastidiosa strain PWT-22, causing periwinkle wilt disease, was constructed using EcoRI, HaeIII, HinfI, MspI, StyI, and TaqI fragments. No restriction site for BamHI, BglI, DraI, EcoRV, HindIII, PstI, SalI, NcoI, or XbaI was evident. Studies based on the intensity of ethidium bromide staining indicated that there are at least 60 copies of pXFPW1 per genome. pXFPW1 shares a high degree homology with two other plasmids from strains PD82-21 and mul1, the pathogens of Pierce's disease of grapevine and mulberry leaf scorch disease, respectively. Key words: Xylella fastidiosa, plasmids.

scholarly journals Infection of Blueberry Cultivar ‘Emerald’ with a California Pierce’s Disease Strain of Xylella fastidiosa and Acquisition by Glassy-Winged Sharpshooter

Plant Disease ◽  
2020 ◽  
Vol 104 (1) ◽  
pp. 154-160
Author(s):  
Lindsey P. Burbank ◽  
Mark S. Sisterson ◽  
Michael L. O’Leary
Keyword(s):  
United States ◽  
Xylella Fastidiosa ◽  
Southeastern United States ◽  
San Joaquin Valley ◽  
Pierce's Disease ◽  
Highbush Blueberry ◽  
Homalodisca Vitripennis ◽  
Pierce’S Disease ◽  
Fastidiosa Strain ◽  
Southern Highbush

Bacterial leaf scorch disease caused by Xylella fastidiosa occurs in southern highbush blueberry varieties in the southeastern United States. Susceptibility to X. fastidiosa varies by blueberry cultivar, and these interactions are often strain-specific. Xylella fastidiosa subsp. fastidiosa is the causal agent of Pierce’s disease in grapevines, and it has been problematic in the San Joaquin Valley of California since the introduction of the glassy-winged sharpshooter (Homalodisca vitripennis). The glassy-winged sharpshooter is known to feed on blueberry, a crop that is expanding in the San Joaquin Valley. Currently, little is known about the potential for the spread of X. fastidiosa between grape and blueberry in this region. The ability of a Pierce’s disease strain of X. fastidiosa from the San Joaquin Valley to cause disease in southern highbush blueberry and the potential for the glassy-winged sharpshooter to transmit X. fastidiosa between blueberry and grapevine were investigated. Experimental inoculations showed that the X. fastidiosa subsp. fastidiosa strain Bakersfield-1 can cause disease in blueberry cv. Emerald, and that the glassy-winged sharpshooter can acquire X. fastidiosa from artificially inoculated blueberry plants under laboratory conditions. Understanding the possibility for X. fastidiosa strains from the San Joaquin Valley to infect multiple crops grown in proximity is important for area-wide pest and disease management.

Biological Control of Citrus Huanglongbing with EB92-1, a Benign Strain of Xylella Fastidiosa

Plant Disease ◽  
2021 ◽  
Author(s):  
Donald Hopkins ◽  
Kelly Wall
Keyword(s):  
Biological Control ◽  
Xylella Fastidiosa ◽  
Good Control ◽  
Candidatus Liberibacter Asiaticus ◽  
Mature Trees ◽  
Fastidiosa Strain ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus ◽  
Old Trees

Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus, is currently the most destructive disease of citrus and threatens production in all affected areas. There is no cure. Benign Xylella fastidiosa strain EB92-1 provided good control of Pierce’s disease of grapevine by inducing host resistance; therefore, we evaluated the strain for the biological control of HLB in citrus. Treatment was by injection into the trunk of the trees. Strain EB92-1 was shown to colonize citrus and provided control of HLB by reducing both the incidence of symptomatic trees and the percentage of trees with severe symptoms in three separate trials. All trees were positive by qPCR for Candidatus Liberibacter asiaticus at the start of the trials and treatment did not eliminate the bacterium. In a trial of asymptomatic mature trees, a single treatment with EB92-1 reduced the incidence of trees with symptoms through 18 months after treatment and reduced the incidence of trees with severe symptoms through 3 years. In mature trees that had 60% incidence of mild HLB symptoms at trial initiation, percentage of trees with symptoms and trees with severe symptoms continued to develop in both the untreated and in the EB92-1 treated trees for 12 months. However, re-treatment at 9 months and 20 months prevented the development of additional severe symptoms in the EB92-1 treated trees throughout the remainder of the 5-year trial. In 2-year-old trees, incidence of trees with HLB symptoms was higher in the untreated trees than in the treated trees throughout the 6 years of the trial, reaching 90% in the untreated versus 50% in the treated. After 6 years, severity of symptoms was much lower in the EB92-1 treated trees, only 3% of the treated trees had become unproductive compared to 18% of the untreated. In 2020, the reduction in HLB severity resulted in approximately twice as much yield in the treated trees as in these untreated 8-year-old trees. Xylella fastidiosa strain EB92-1 proved to be efficacious for the control of HLB symptoms in both mature trees and newly planted young trees, but trees may need to be re-treated yearly for at least the first 2-3 years.

scholarly journals Host Specificity of Pecan Strains of Xylella fastidiosa subsp. multiplex

Plant Disease ◽  
2017 ◽  
Vol 101 (5) ◽  
pp. 744-750 ◽  
Author(s):  
R. S. Sanderlin
Keyword(s):  
Management Practices ◽  
Clonal Propagation ◽  
Xylella Fastidiosa ◽  
Host Specialization ◽  
Broad Host Range ◽  
Red Maple ◽  
Fastidiosa Strain ◽  
Hardwood Tree ◽  
Management Recommendations ◽  
Greenhouse Tests

Pecan (Carya illinoinensis) bacterial leaf scorch disease, caused by Xylella fastidiosa subsp. multiplex, causes defoliation and reduces terminal growth and nut yield. The pathogen is transmitted to pecan by xylem-feeding spittlebugs and leafhoppers and through graft transmission in the clonal propagation of cultivars. Xylella fastidiosa subsp. multiplex has a broad host range, infecting numerous hardwood tree species and some herbaceous species. There is evidence of additional host specialization within subsp. multiplex. Data presented here support the existence of host specialization with X. fastidiosa that infect pecan. In this study, mechanical inoculation was used to inoculate several plant species that are naturally infected by subsp. multiplex, including sycamore, red maple, purple-leafed plum, and blueberry with strains of X. fastidiosa from pecan. Hosts of three other subspecies were also inoculated with the pecan strains: grapevine (subsp. fastidiosa); oleander (subsp. sandyi); and mulberry (subsp. morus). Pecan was also inoculated with a strain of the pathogen from sycamore (subsp. multiplex) and a strain from grapevine (subsp. fastidiosa). In greenhouse tests, inoculum prepared from X. fastidiosa obtained from naturally infected pecan almost exclusively infected pecan. In addition, the subsp. multiplex strain from sycamore generally did not infect pecan, and the subsp. fastidiosa strain from grapevine did not infect pecan. The inability of the pecan strain to readily infect other hosts commonly located in the vicinity of pecan orchards affects the management recommendations for the disease in commercial pecan production by allowing management practices to focus on pecan orchards and insect vectors.

scholarly journals The Importance of Multilocus Sequence Typing: Cautionary Tales from the Bacterium Xylella fastidiosa

2012 ◽  
Vol 102 (5) ◽  
pp. 456-460 ◽  
Author(s):  
L. Nunney ◽  
S. Elfekih ◽  
R. Stouthamer
Keyword(s):  
Multilocus Sequence Typing ◽  
Evolutionary Dynamics ◽  
Xylella Fastidiosa ◽  
Bacterial Species ◽  
Culture Collection ◽  
Genotypic Differences ◽  
Culture Collections ◽  
Microbial Identification ◽  
Fastidiosa Strain ◽  
Cautionary Tales

Microbial identification methods have evolved rapidly over the last few decades. One such method is multilocus sequence typing (MLST). MLST is a powerful tool for understanding the evolutionary dynamics of pathogens and to gain insight into their genetic diversity. We illustrate the importance of accurate typing by reporting on three problems that have arisen in the study of a single bacterial species, the plant pathogen Xylella fastidiosa. Two of these were particularly serious since they concerned contamination of important research material that has had detrimental consequences for Xylella research: the contamination of DNA used in the sequencing of an X. fastidiosa genome (Ann-1) with DNA from another X. fastidiosa strain, and the unrecognized mislabeling of a strain (Temecula1) distributed from a culture collection (ATCC). We advocate the routine use of MLST to define strains maintained in culture collections and emphasize the importance of confirming the purity of DNA submitted for sequencing. We also present a third example that illustrates the value of MLST in guiding the choice of taxonomic types. Beyond these situations, there is a strong case for MLST whenever an isolate is used experimentally, especially where genotypic differences are suspected to influence the outcome.

scholarly journals Draft Genome Sequence of Xylella fastidiosa subsp. fastidiosa Strain IVIA5235, Isolated from Prunus avium in Mallorca Island, Spain

2018 ◽  
Vol 7 (14) ◽  
Author(s):  
Blanca B. Landa ◽  
María P. Velasco-Amo ◽  
Ester Marco-Noales ◽  
Diego Olmo ◽  
María M. López ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Prunus Avium ◽  
Xylella Fastidiosa ◽  
Draft Genome ◽  
Pathogenic Bacterium ◽  
Draft Genome Sequence ◽  
Plant Pathogenic Bacterium ◽  
Content Type ◽  
Fastidiosa Strain ◽  
Mallorca Island

We report the complete annotated genome sequence of the plant-pathogenic bacterium Xylella fastidiosa subsp. fastidiosa strain IVIA5235.

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