scholarly journals El papel de los procariotas en la degradación del cianuro

Herreriana ◽  
2022 ◽  
Vol 3 (2) ◽  
pp. 33-36
Author(s):  
Sylvia Martinez ◽  
Andrea Aguilar Hernández
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Fluorescens ◽  
Bacillus Sp

El cianuro es una sustancia tóxica, potencialmente letal, cuya concentración ha incrementado debido a las actividades antropogénicas, lo que genera un impacto negativo en el ecosistema y en la salud de los organismos expuestos. Desde hace un par de décadas se ha utilizado un proceso biotecnológico llamado “biorremediación”, que consiste en aprovechar las capacidades metabólicas de los microorganismos como bacterias y arqueas que degradan o atenúan contaminantes como el cianuro y los compuestos que forma. El presente trabajo tiene como objetivo identificar a las principales especies de bacterias y arqueas con capacidad para degradar el cianuro en sus distintas formas mediante un análisis bibliográfico. Dentro de los microorganismos utilizados para la degradación de cianuro se encuentran Bacillus sp., Escherichia coli, Pseudomonas fluorescens, entre otras especies. Con base en el número de especies descritas y sus eficientes tasas de remoción, se determinó que el proceso de biorremediación del cianuro es una alternativa prometedora, además existe una mayor degradación en el proceso aeróbico comparado con el proceso anaeróbico.

scholarly journals A novel chitosanase from Bacillus sp. Strain K17: Gene cloning and expression in Escherichia coli

2002 ◽  
Vol 2 (1) ◽  
pp. 227-228 ◽  
Author(s):  
R. Yatsunami ◽  
Y. Sakihama ◽  
M. Suzuki ◽  
T. Fukazawa ◽  
S. Shimizu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Cloning ◽  
Cloning And Expression ◽  
Gene Cloning And Expression ◽  
Bacillus Sp ◽  
Expression In Escherichia Coli

scholarly journals ISOLASI BAKTERI DARI TANAH SEBAGAI PENGHASIL SENYAWA ANTIMIKROB

Biospecies ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 46-51
Author(s):  
Sukmawati Sukmawati ◽  
FEBRIANTI ROSALINA
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Sp ◽  
E Coli

Antibiotik adalah bahan obat yang sangat memegang peranan penting dalam menanggulangi penyakit infeksi. Senyawa antimikroba dapat diperoleh dari tumbuh-tumbuhan, dan mikroba. Senyawa antimikrob yang dihasilkan oleh mikroba memiliki keunggulan dibandingkan dengan antibiotik sintetik karena memiliki sifat yang lebih efektif, sebab targetnya spesifik serta toksisitasnya rendah. Tujuan dari penelitian yang telah dilakukan ialah untuk mengisolasi bakteri dari tanah yang mampu menghasilkan senyawa antimikrob, serta untuk menguji aktivitas penghambtannya terhadap  pertumbuhan Escherichia coli dan Staphylococcus aureus. Metode yang telah digunakan dalam penelitian ini ialah isolasi, purifikasi, dan seleksi bakteri dari sampel tanah dengan metode uji penghambatan sedangkan bakteri Bacillus sp. Merupakan bakteri pembanding. Berdasarkan hasil penelitian yang diperoleh terdapat 2 isolat yang berpotensi memiliki aktivitas antimikroba yaitu isolat 1 dan isolate 4. Isolat 1 lebih berpotensi menghambat E. coli dengan indeks hambat 4.0 mm dibandingkan dengan penghambatan S. aureus dengan indeks hambat 3.1 mm. Sedangkan isolat 4 lebih berpotensi menghambat S. aureus dengan indeks hambat 2.8 mm dibandingkan dengan penghambatan terhadap E. coli dengan indeks hambat 1.4 mm.

scholarly journals Enhanced citric acid biosynthesis in Pseudomonas fluorescens ATCC 13525 by overexpression of the Escherichia coli citrate synthase gene

Microbiology ◽  
2009 ◽  
Vol 155 (8) ◽  
pp. 2620-2629 ◽  
Author(s):  
Aditi D. Buch ◽  
G. Archana ◽  
G. Naresh Kumar
Keyword(s):  
Escherichia Coli ◽  
Citric Acid ◽  
Acid Secretion ◽  
Pseudomonas Fluorescens ◽  
Phosphoenolpyruvate Carboxylase ◽  
Citrate Synthase ◽  
Acid Biosynthesis ◽  
Acid Yield ◽  
Glucose Catabolism ◽  
Citric Acid Biosynthesis

Citric acid secretion by fluorescent pseudomonads has a distinct significance in microbial phosphate solubilization. The role of citrate synthase in citric acid biosynthesis and glucose catabolism in pseudomonads was investigated by overexpressing the Escherichia coli citrate synthase (gltA) gene in Pseudomonas fluorescens ATCC 13525. The resultant ∼2-fold increase in citrate synthase activity in the gltA-overexpressing strain Pf(pAB7) enhanced the intracellular and extracellular citric acid yields during the stationary phase, by about 2- and 26-fold, respectively, as compared to the control, without affecting the growth rate, glucose depletion rate or biomass yield. Decreased glucose consumption was paralleled by increased gluconic acid production due to an increase in glucose dehydrogenase activity. While the extracellular acetic acid yield increased in Pf(pAB7), pyruvic acid secretion decreased, correlating with an increase in pyruvate carboxylase activity and suggesting an increased demand for the anabolic precursor oxaloacetate. Activities of two other key enzymes, glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase, remained unaltered, and the contribution of phosphoenolpyruvate carboxylase and isocitrate lyase to glucose catabolism was negligible. Strain Pf(pAB7) demonstrated an enhanced phosphate-solubilizing ability compared to the control. Co-expression of the Synechococcus elongatus PCC 6301 phosphoenolpyruvate carboxylase and E. coli gltA genes in P. fluorescens ATCC 13525, so as to supplement oxaloacetate for citrate biosynthesis, neither significantly affected citrate biosynthesis nor caused any change in the other physiological and biochemical parameters measured, despite approximately 1.3- and 5-fold increases in citrate synthase and phosphoenolpyruvate carboxylase activities, respectively. Thus, our results demonstrate that citrate synthase is rate-limiting in enhancing citrate biosynthesis in P. fluorescens ATCC 13525. Significantly low extracellular citrate levels as compared to the intracellular levels in Pf(pAB7) suggested a probable limitation of efficient citrate transport.

The N-Terminal Signal Sequence and the Last 98 Amino Acids Are Not Essential for the Secretion of Bacillus sp. TS-23 α-Amylase in Escherichia coli

2001 ◽  
Vol 43 (3) ◽  
pp. 170-175 ◽  
Author(s):  
Huei-Fen Lo ◽  
Long-Liu Lin ◽  
Chien-Cheng Li ◽  
Wen-Hwei Hsu ◽  
Chen-Tien Chang
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Signal Sequence ◽  
Bacillus Sp
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