scholarly journals A Methodical Approach to Interpreting the White Blood Cell Parameters of the Complete Blood Count

2017 ◽  
Vol 30 (3) ◽  
pp. 186-193
Author(s):  
Kathy Doig ◽  
Leslie A. Thompson
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Blood Count ◽  
Complete Blood Count ◽  
Cell Parameters ◽  
Methodical Approach

scholarly journals A Novel Automated Slide-Based Technology for Visualization, Counting, and Characterization of the Formed Elements of Blood: A Proof of Concept Study

2017 ◽  
Vol 141 (8) ◽  
pp. 1107-1112 ◽  
Author(s):  
James W. Winkelman ◽  
Milenko J. Tanasijevic ◽  
David J. Zahniser
Keyword(s):  
Image Analysis ◽  
Blood Cell ◽  
White Blood Cell ◽  
Blood Count ◽  
Complete Blood Count ◽  
Differential Count ◽  
Preparation Technique ◽  
Proof Of Concept ◽  
New Instrument ◽  
Prototype Instrument

Context.— A novel automated slide-based approach to the complete blood count and white blood cell differential count is introduced. Objective.— To present proof of concept for an image-based approach to complete blood count, based on a new slide preparation technique. A preliminary data comparison with the current flow-based technology is shown. Design.— A prototype instrument uses a proprietary method and technology to deposit a precise volume of undiluted peripheral whole blood in a monolayer onto a glass microscope slide so that every cell can be distinguished, counted, and imaged. The slide is stained, and then multispectral image analysis is used to measure the complete blood count parameters. Images from a 600-cell white blood cell differential count, as well as 5000 red blood cells and a variable number of platelets, that are present in 600 high-power fields are made available for a technologist to view on a computer screen. An initial comparison of the basic complete blood count parameters was performed, comparing 1857 specimens on both the new instrument and a flow-based hematology analyzer. Results.— Excellent correlations were obtained between the prototype instrument and a flow-based system. The primary parameters of white blood cell, red blood cell, and platelet counts resulted in correlation coefficients (r) of 0.99, 0.99, and 0.98, respectively. Other indices included hemoglobin (r = 0.99), hematocrit (r = 0.99), mean cellular volume (r = 0.90), mean corpuscular hemoglobin (r = 0.97), and mean platelet volume (r = 0.87). For the automated white blood cell differential counts, r values were calculated for neutrophils (r = 0.98), lymphocytes (r = 0.97), monocytes (r = 0.76), eosinophils (r = 0.96), and basophils (r = 0.63). Conclusions.— Quantitative results for components of the complete blood count and automated white blood cell differential count can be developed by image analysis of a monolayer preparation of a known volume of peripheral blood.

scholarly journals Evaluation Of Complete Blood Count Parameters To Predict Ovarian Torsion In Women With Adnexal Mass

2017 ◽  
pp. 1 ◽  
Author(s):  
Ahter T. Tayyar ◽  
Enis Özkaya ◽  
Çiğdem Abide Yayla ◽  
Mehmet Baki Şentürk ◽  
Selçuk Selçuk ◽  
...  
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Neutrophil Count ◽  
Ovarian Cyst ◽  
Blood Count ◽  
Complete Blood Count ◽  
Adnexal Mass ◽  
Ovarian Torsion ◽  
Adnexal Torsion ◽  
Neutrophil Lymphocyte Ratio

<p><strong>Objective:</strong> The aim of this study was to evaluate complete blood count parameters to predict ovarian torsion in cases presented with ovarian mass.</p><p><strong>Study Design:</strong> Pre-operative demographic data and complete blood count parameters of 72 patients, who were operated on preliminary adnexal torsion and diagnosed as adnexal torsion with a benign ovarian cyst (Study group) were retrospectively compared with those of 77 patients who were operated with an indication of persistent benign ovarian cysts without torsion (control group) at Zeynep Kamil Women and Children’s Health Training and Research Hospital and Department of Obstetrics &amp; Gynecology at Erciyes University Medical Faculty between 2011 and 2015. Complete blood count parameters were utilized to predict ovarian torsion cases.</p><p><strong>Result:</strong> Neutrophil (AUC=792, P=&lt;0.001), white blood cell (AUC=787, P=&lt;0.001) counts and neutrophil/lymphocyte ratio (AUC=770, P=&lt;0.001) were significant predictors for adnexal torsion. Optimal cut off value for white blood cell, neutrophil count and neutrophil/lymphocyte ratio were 8.3x103 (72% sensitivity, 73% specificity), 5.5x103 (73% sensitivity, 76% specificity), 2.9 (73% sensitivity, 79% specificity) respectively.</p><p><strong>Conclusion:</strong> Among all the parameters white blood cell count, neutrophil/lymphocyte and neutrophil count were the most powerful predictors for real adnexal torsion cases. Simple blood count parameters detailed evaluation may help clinicians to confirm or rule out adnexal torsion in cases presented with ovarian cyst and adnexal mass.</p>

scholarly journals 296 Effects of corticotropin-releasing hormone (CRH) and vasopressin (VP) challenge duration on complete blood count and serum metabolites of growing beef cattle

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 136-137
Author(s):  
Kendall Samuelson ◽  
Meagan D Wright ◽  
John T Richeson ◽  
Jennifer A Hernandez Gifford ◽  
Nicole C Sanchez
Keyword(s):  
Blood Cell ◽  
Beef Cattle ◽  
White Blood Cell ◽  
Rectal Temperature ◽  
Blood Count ◽  
Complete Blood Count ◽  
Block Design ◽  
Serum Glucose ◽  
Serum Metabolites ◽  
And Control

Abstract The objective was to evaluate stress challenge and duration on immunologic and metabolic variables of growing beef cattle. Thirty crossbred steers were used in a randomized complete block design. Treatments were i.v. injection of: 1) Control (3 mL saline for 4 consecutive days: n = 10), 2) Acute (0.3 µg/kg BW CRH and 1.0 µg/kg BW VP for 1 day, saline for 3 consecutive days; n = 10), or 3) Chronic (0.3 µg/kg BW CRH and 1.0 µg/kg BW VP for 4 consecutive days; n = 10) at hour 0, 24, 48, and 72. Rectal temperature and serum were collected at hour 0, 1, 2, 24, 25, 26, 48, 49, 50, 72, 73, 74, 144, and 336 for analysis of cortisol and serum metabolites. Whole blood was collected at hour 0, 24, 48, 72, 144, and 336 for analysis of complete blood count. Data were analyzed using the MIXED procedure of SAS. Rectal temperature tended to be greater for stress challenged cattle (stress vs. no; P = 0.06). Cortisol was greater for cattle receiving acute and chronic than control at hour 1 and greater for chronic than both acute and control at hour 25, 26, 49, 50, 73, and 74 (P ≤ 0.01). Similarly, serum glucose concentration was increased at hour 1 for acute and chronic and chronic had greater glucose at hour 25, 49, 50, 73, and 74 (P ≤ 0.04). Potassium was less for acute and chronic at hour 1 and 2 and chronic was less than acute and control at hour 24, 25, 26, 49, 50, 73, and 74 (P ≤ 0.02). Chronic had greater (P = 0.04) white blood cell count than control at 72 h. Monocyte concentration was least at hour 144 for chronic (P &lt; 0.01). Results demonstrate that stress influences cortisol, glucose, electrolyte, and white blood cell concentrations.

scholarly journals Averrhoa bilimbi Extract as an Alternative Anticoagulant for Manual Complete Blood Count

2019 ◽  
Vol 5 (1) ◽  
pp. 6
Author(s):  
Arvyl Jan L. Andaya ◽  
Prince Duncan C. Maylas ◽  
Ma. Estrella H. Sales
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Red Blood Cell ◽  
Blood Count ◽  
Clinical Laboratory ◽  
Complete Blood Count ◽  
Ethylenediaminetetraacetic Acid ◽  
Wbc Count ◽  
Edta Anticoagulated Blood ◽  
Rbc Count

This study was designed to determine whether Averroha bilimbi extract can be used as an alternative anticoagulant for manual complete blood count (CBC) in the hematology clinical laboratory instead of Ethylenediaminetetraacetic acid (EDTA), the recommended anticoagulant for CBC. Blood from 15 volunteers was extracted and placed in EDTA-anticoagulated tubes and tubes with Averrhoa bilimbi extract. Samples from both tubes were tested for CBC. Using independent t-test the study revealed that there is no difference in the red blood cell (RBC) count, white blood cell (WBC) count, hemoglobin, hematocrit, and a 3-part differential of EDTA anticoagulated blood and blood with Averrhoa bilimbi extract as anticoagulant. The morphology of lymphocytes and monocytes were not affected, however, the granulocytes showed cytoplasmic distortion and vacuolation in the Averrhoa bilimbi extract.

scholarly journals Surgical Treatment of Sialolithiasis Leads to Improvement in the Complete Blood Count

Biology ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 414
Author(s):  
Gal Avishai ◽  
Idan Rabinovich ◽  
Hanna Gilat ◽  
Gavriel Chaushu ◽  
Liat Chaushu
Keyword(s):  
Surgical Treatment ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Count ◽  
Complete Blood Count ◽  
Blood Cell Count ◽  
Blood Profile ◽  
Post Procedure ◽  
Red Blood Cell Count ◽  
Anemia Of Inflammation

Sialolithiasis is a chronic disease in which a sialolith (salivary stone) causes recurrent inflammation of the affected salivary gland. Anemia of inflammation is a well-described pathology in which a chronic inflammatory disease leads to a reduction in the red blood cell count, hemoglobin and hematocrit values. In this retrospective cohort study, we aim to find whether removal of the sialolith and alleviation of the inflammation affect the complete blood count results. We examined data regarding forty-nine patients who underwent surgery for the removal of a submandibular gland sialolith using the duct-stretching technique. Complete blood counts two years before and after the surgical procedure were collected. The average pre-procedure and post-procedure values were calculated for each patient to establish the average blood profile. The pre- and post-procedure values were compared to evaluate the effect of the surgical treatment on the blood profile. We found that the average blood count values for patients with sialolithiasis were towards the lower end of the normal range. Post-surgery, a significant increase in hematocrit, hemoglobin and red blood cell count was observed, which was more pronounced in the older age group and in patients with co-morbidities. We conclude that sialolith removal surgery is associated with significant improvement in the complete blood count values, especially in the elderly and in patients and with co-morbidities. The speculated pathogenesis is relative anemia of inflammation.

Sign in / Sign up

Export Citation Format

Share Document