Enhanced production of extracellular lipase by ethyl methane sulfonate from soil fungal strain in submerged and solid-state fermentation condition

Enhancement in the production of enzyme by utilizing different strains of microbe is one of the main prospects in biotechnology. In the present work, ethyl methane sulfonate (EMF) was selected as the chemical mutagen for inducing mutagenesis in fungi. It is a cheap method to induce random mutation as compared to other methods of recombinant technologies. Strain improvement was done by incubating the fungal spore suspension at variable concentrations of EMS i.e. 4% (v/v) and 10% (v/v) for the time period of 60, 90, and 120 min respectively. The set of control was treated with distilled water only. The fungal colonies were found to be maximum in control plate as compared to the EMF exposed plates. The number of fungal colonies was reduced as we raised the exposure time of EMF. Specific activity and the lipase activity of wild strain and hyperproducer were evaluated under the submerged (SmF) and solid-state fermentation (SSF). The wild strain denoted the 3.2 U/ml/min of enzymatic activity under SmF and 15.87 U/g/min of activity under SSF. In contrast, the best enzymatic activity was represented by S2St1 at 10% of EMS after the time period of 60 min i.e. 11.7 U/ml/min under SmF and 99.35 U/g/min under SSF after the time period of 72 hrs. Statistical analysis by using one-way ANOVA determined that the value of F calculated was lower than the F tabulated. So, there was a significant relation between the EMS percentage and time of exposure among the mutated strains. In conclusion, this soil fungal strain can be utilized to produce lipase enzyme for numerous industrial applications.

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Strain Improvement of Streptomyces venezuelae for Enhanced Fibrinolytic Enzyme Production

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.584.440 ◽

2012 ◽

Vol 584 ◽

pp. 440-444 ◽

Cited By ~ 2

Author(s):

Balasubramanian Bhavani ◽

Balakrishnan Naveena ◽

Nagarajan Partha

Keyword(s):

Fibrinolytic Activity ◽

Wild Strain ◽

Strain Improvement ◽

Fibrinolytic Enzyme ◽

Future Application ◽

Ethyl Methane Sulfonate ◽

Streptomyces Venezuelae ◽

Methane Sulfonate ◽

Fibrinolytic Enzymes ◽

Ethyl Methane

The fibrinolytic enzymes can be used as a potential drug to cure thrombosis diseases. These enzymes can effectively catalyze the degradation of fibrin in blood clot. To develop safe and cheaper fibrinolytic agents, fibrinolytic enzyme was isolated from Streptomyces venezuelae. Strain improvement was employed to increase the production of fibrinolytic enzymes using random mutagenesis (UV and Ethyl methane sulfonate). The mutants obtained were screened based on their fibrinolytic activity and best mutant was selected for further studies. Mutant obtained by Ethyl Methane Sulfonate was able to yield the fibrinolytic activity of 13 FU/mL in growth medium which was higher than wild strain (6 FU/mL). The results indicated that EMS was effective mutagenic agents for strain improvement of Streptomyces venezuelae for enhanced production of fibrinolytic enzyme. The mutant showed improved growth compared to wild strain. The optimal temperature and pH value of this fibrinolytic enzyme were found to be 40 °C and 8.0, respectively. The strain improvement also improves the stability of Streptomyces venezuelae which showed resistance to temperature and pH at higher values. Invitro assays revealed that fibrinolytic enzyme produced by Streptomyces venezuelae could degrade fibrin suggesting that its future application in pharmaceutical industry as thrombolytic agent is highly promising.

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Enhancement of lipase production by ethyl methane sulfonate mutagenesis of soil fungal isolate

Plant Science Today ◽

10.14719/pst.2019.6.sp1.674 ◽

2019 ◽

Vol 6 (sp1) ◽

pp. 600-606 ◽

Cited By ~ 1

Author(s):

` Shreya ◽

Arun Kumar Sharma ◽

` Ritika ◽

Nikita Bhati

Keyword(s):

Wild Strain ◽

Random Mutagenesis ◽

Strain Improvement ◽

Lipase Production ◽

Maximum Activity ◽

Spore Suspension ◽

Ethyl Methane Sulfonate ◽

Water Control ◽

Methane Sulfonate ◽

Ethyl Methane

Strain improvement through random mutagenesis is an extremely developed practice and it plays an important role in the economic growth of microbial agitation processes. The present study comprises of genetic improvement of fungus isolated from petrol pump soil by ethyl methane sulfonate (EMS) mutagenesis for increased production of extracellular lipase. Random mutagenesis was performed by incubating the spore suspension of fungus with EMS at a concentration of 5% (v/v) and 8% (v/v) for 30, 60 and 90 min, respectively. Control set was prepared by incubating the spore suspension with sterile distilled water. Control plate showed maximum number of fungal colonies whereas number of colonies was decreased as we increased exposure time of EMS from 30 to 90 min. The lipase activity of six mutagenic strains and wild strain was determined under submerged fermentation and solid state fermentation. Treated culture named as EMS5%-60min (obtained after 60 min exposure with 5% EMS) exhibited maximum activity (32.09 ± 1.84 IU/ml/min) in SmF as compared to wild strain (8.77 ± 3.52 IU/ml/min) and another treated strain named as EMS8%-90min (obtained after 90 min exposure with 8% EMS) exhibited maximum activity (7.99 ± 0.12 IU/g/min) in SSF as compared to wild strain (1.77 ± 0.71 IU/g/min). The activity of mutagenic strain i.e. EMS5%-60min was increased to 365.90% as compared to 100% activity of wild strain in SmF whereas activity of another mutagenic strain i.e. EMS8%-90min was increased to 451.41% as compared to 100% activity of wild strain in SSF.

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PERLAKUAN ETHYL METHANE SULFONATE (EMS) PADA ENTEROBACTER AEROGENES AY-2 DARI LIMBAH METAN FERMENTASI UNTUK PENINGKATAN PRODUKSI GAS HIDROGEN

Jurnal Rekayasa Lingkungan ◽

10.29122/jrl.v5i1.1875 ◽

2018 ◽

Vol 5 (1) ◽

Author(s):

Mahyudin Abdul Rachman

Keyword(s):

Metabolic Pathway ◽

Acid Production ◽

Double Mutant ◽

Enterobacter Aerogenes ◽

H2 Production ◽

Hydrogen Gas ◽

Survival Ratio ◽

Ethyl Methane Sulfonate ◽

Methane Sulfonate ◽

Ethyl Methane

Enterobacter aerogenes AY-2 mutant is known for hydrogen gas producer which ws obtained from the sludge of methane fermentation and the yield is 1.5 fold higher than wildtype. Hydrogen gas production can be gain via NADH oxidation in anaerobic metabolic pathway by blocking organic acid production. Metabolic pathway can be changed by mutagenesis. Enterobacter aerogenes AY-2 mutated with ethyl methane sulfonate in logarithmic phase with consentration 10, 11, 12, 13, 14 and 15 μl/ml cell suspention during 120 minute. Mutation that result lowest survival ratio (0,01%) was 14 μl EMS/ml cell suspention is repeated with variation incubation time, 30, 60, 90 and 120 minute. 166 double mutant colony has been collected and choosen randomly. The choosen 43 colony was fermented in glycerol complex medium for determining ten double mutant with the highest H2 production. Double mutant AD-H43 is a highest H2 producer that increase 20% H2 production from AY-2 and has a decrease lactid acid production, 31% less from AY-2. Increasing H2 production in double mutant AD-H43 is caused by lactate dehydrogenase deffi cient.Keywords: Enterobacter aerogenes AY-2, ethyl methane sulfonate (EMS), H2 and methane sludge

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Effectiveness and efficiency of electron beam in comparison with gamma rays and ethyl methane sulfonate mutagens in cowpea

Applied Radiation and Isotopes ◽

10.1016/j.apradiso.2021.109640 ◽

2021 ◽

Vol 171 ◽

pp. 109640

Author(s):

Vijayakumar Eswaramoorthy ◽

Thangaraj Kandasamy ◽

Kalaimagal Thiyagarajan ◽

Chockalingam Vanniarajan ◽

Souframanien Jegadeesan

Keyword(s):

Electron Beam ◽

Gamma Rays ◽

Ethyl Methane Sulfonate ◽

Methane Sulfonate ◽

Ethyl Methane ◽

Effectiveness And Efficiency

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EXTRACHROMOSOMAL ELEMENT DELTA IN DROSOPHILA MELANOGASTER. IX. INDUCTION OF DELTA-RETAINING CHROMOSOME LINES BY MUTATION AND GENE MAPPING

Genetics ◽

10.1093/genetics/74.3.477 ◽

1973 ◽

Vol 74 (3) ◽

pp. 477-487

Author(s):

Sumio Minamori ◽

Kinue Sugimoto

Keyword(s):

Drosophila Melanogaster ◽

Crossing Over ◽

Chromosomal Gene ◽

Ethyl Methane Sulfonate ◽

Methane Sulfonate ◽

Multiple Alleles ◽

Ethyl Methane ◽

Extrachromosomal Element ◽

Polygenic System ◽

Killing Action

ABSTRACT [Delta b], symbolized as [δb], is retained by Sb chromosome lines and transmitted through the females to their progeny. Transmission through the males is not directly demonstrable (Minamori 1969a). [delta r], symbolized as [δr], is retained by Sr chromosome lines and transmitted biparentally (Minamori 1971). The multiplication of delta is suppressed at low temperature. All descendant lines derived from Sb-carrying or Sr-carrying flies in which the presence of delta cannot be demonstrated gradually accumulate their specific delta factors over many generations (Minamori 1969b, 1972). The delta factors and the sensitive chromosomes are inseparably associated. This observation led to the assumption that delta may be a copy of a chromosomal gene or a certain agent integrated into the chromosome (Minamori 1972). This assumption was examined in the present study by experiments designed to induce delta-retaining sensitive chromosomes, and to map the gene(s) responsible for delta-retention and/or for sensitivity to the killing action of delta factor. One sensitive chromosome which retained [δb] (Sb chromosome) was obtained in the presence of [δb] out of 2492 insensitive chromosomes which retained no delta; in addition one Sb chromosome was obtained in the presence of [δr] out of 2131 insensitives. The latter finding suggests that Sb might be induced by a mutation caused by [δb] or [δr], but not by integration of either delta into the chromosome. Four Sb chromosomes and one sensitive chromosome which retained [δr] (Sr chromosome) were obtained out of 1970 insensitives when males carrying the chromosome were fed an alkylating mutagen, ethyl methane sulfonate (EMS). The location of delta-retaining genes was examined by crossing-over experiments employing eight Sb and five Sr chromosomes. The genes on these chromosomes were found to be located in the same region or near one another. The gene for [δb], symbolized as Dab, and the gene for [δr], symbolized as Dar, are assumed to be multiple alleles of a locus at 2-24.9. The sensitivity of the chromosomes was modified appreciably by recombination; hence, the genes controlling this trait are assumed to be a polygenic system. The findings obtained in this study lead to the hypothesis that delta may be produced by a chromosomal gene (Da) and transmitted extrachromosomally.

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Phenotypic variation analysis of mutant population of ethyl methane sulfonate in peppers

Pakistan Journal of Botany ◽

10.30848/pjb2022-1(9) ◽

2021 ◽

Vol 54 (1) ◽

Author(s):

Huang Dongfu ◽

He Jianwen ◽

Fu Wenting ◽

Hu Mingwen ◽

Yang Hong

Keyword(s):

Phenotypic Variation ◽

Ethyl Methane Sulfonate ◽

Variation Analysis ◽

Methane Sulfonate ◽

Ethyl Methane ◽

Mutant Population

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Ethyl Methane Sulfonate Enhances Genetic Variability in Capsicum annuum

Asian Journal of Plant Sciences ◽

10.3923/ajps.2002.425.428 ◽

2002 ◽

Vol 1 (4) ◽

pp. 425-428 ◽

Cited By ~ 7

Author(s):

Nyla Jabeen ◽

Bushra Mirza .

Keyword(s):

Genetic Variability ◽

Capsicum Annuum ◽

Ethyl Methane Sulfonate ◽

Methane Sulfonate ◽

Ethyl Methane

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Curly Stem – an Induced Mutation in Flax (Linum usitatissimum L.)

Czech Journal of Genetics and Plant Breeding ◽

10.17221/6246-cjgpb ◽

2012 ◽

Vol 38 (No. 3-4) ◽

pp. 125-128 ◽

Cited By ~ 5

Author(s):

E. Tejklová

Keyword(s):

Linum Usitatissimum ◽

Wild Type Allele ◽

Ethyl Methane Sulfonate ◽

Wild Type ◽

Methane Sulfonate ◽

Ethyl Methane ◽

Straight Stem ◽

Linum Usitatissimum L ◽

Factors Influencing ◽

Stem Shape

After ethyl methane sulfonate (EMS) treatment of two flax lines, curly stem mutations appeared in both, besides of other mutations. Genetic analysis of one CS mutant line confirmed a monogenic inheritance of the changed stem shape. The curly stem allele is partially dominant over the wild type allele for straight stem. Homozygotic mutants have a curly stem, heterozygotic plants have a flexuous stem, while the stem of homozygotic recessive plants is straight. The expression of the curly stem character is affected by factors influencing plant growth. The utilisation of this mutation for ornamental and other purposes is considered.

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