scholarly journals An Optimized Protocol for Agrobacterium rhizogenes-Mediated Genetic Transformation of Citrullus colocynthis

2019 ◽  
Vol 6 (3) ◽  
pp. 113-117 ◽  
Author(s):  
Mina Beigmohammadi ◽  
Ali Sharafi ◽  
Samineh Jafari
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Citrullus Colocynthis ◽  
Optimized Protocol

Improvement of Ornamental Characters and Fragrance Production in Lemon-scented Geranium Through Genetic Transformation by Agrobacterium rhizogenes

1994 ◽  
Vol 12 (1) ◽  
pp. 64-68 ◽  
Author(s):  
Alessandro Pellegrineschi ◽  
Jain-Pierre Damon ◽  
Nathalie Valtorta ◽  
Nicole Paillard ◽  
David Tapfer
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes

An efficient protocol for genetic transformation of watercress (Nasturtium officinale) using Agrobacterium rhizogenes

2010 ◽  
Vol 38 (8) ◽  
pp. 4947-4953 ◽  
Author(s):  
Nam Il Park ◽  
Jae Kwang Kim ◽  
Woo Tae Park ◽  
Jin Woong Cho ◽  
Yong Pyo Lim ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Nasturtium Officinale

Induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes

2013 ◽  
Vol 8 (5) ◽  
pp. 470-479 ◽  
Author(s):  
Mariya Petrova ◽  
Ely Zayova ◽  
Mariana Vlahova
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Hairy Roots ◽  
Culture Media ◽  
Pcr Analysis ◽  
Transformed Roots ◽  
Fresh Weight ◽  
Transformation Techniques ◽  
Nutrient Media ◽  
Arnica Montana

AbstractThe induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes mediated system was established. The frequency of genetic transformation varied from 4.8 to 12% depended on method of infection. The cefotaxime at concentration of 200 mg/l proved to suppress effectively the growth of A. rhizogenes after co-cultivation. Among the three tested nutrient media: Murashige and Skoog (MS), Gamborg’s (B5) and Schenk and Hildebrandt (SH), MS medium was superior for growth and high biomass production of transformed roots compared to other culture media. After culturing for 40 days the fresh weight of clone T4 increased 7.6 fold over the non-transformed roots. The transfer of rol A, rol B and rol C genes into Arnica genome was confirmed by PCR analysis. Established genetic transformation techniques in A. montana efficiently provided and generated a large number of transformed roots — an excellent system for studying gene function and could be used for the production of secondary metabolites synthesized in roots.

scholarly journals SHOOT POSITION, IBA AND AGROBACTERIUM RHIZOGENES INFLUENCE PROPAGATION OF HAZELNUT, CORYLUS AVELLANA

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1150g-1150
Author(s):  
William M. Proebsting ◽  
Nahla V. Bassil ◽  
David A. Lightfoot
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Corylus Avellana ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Influence Propagation ◽  
Rooting Of Cuttings ◽  
Shoot Position

Propagation of Corylus avellana stem cuttings may be limited by either root initiation or bud abscission. We divided juvenile shoots of 3 varieties growing in layering beds in mid-July into 4 or 5 3-node cuttings with leaves at the upper two nodes, except that terminal cuttings had one expanded leaf. Cuttings were treated with 5 mM IBA in 50% EtOH, a mixture of A. rhizogenes strains A7 + 22 or left untreated. IBA and bacteria stimulated rooting of cuttings from all shoot positions. Rooting of the terminal cuttings (<50%) was less than that of the sub-terminal cuttings (>80%). Bud retention was <50% on terminal cuttings, nearly 100% on sub-terminal cuttings. Using juvenile stock plants of various varieties, sub-terminal cuttings treated with Agrobacterium or 5 mM IBA may yield 70-90% cuttings with both roots and buds, Agravitropic roots, characteristic of genetic transformation, were observed on Agrobacterium-treated cuttings. Dot blots probed for TL-DNA were negative, however.

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