Khảo sát điều kiện trích ly và tinh sạch lectin từ đậu ma (Pueraria phaseoloides) bằng sắc ký ái lực

Nghiên cứu được thực hiện nhằm xác định điều kiện trích ly và tinh sạch lectin từ đậu ma Pueraria phaseoloides. Lectin đậu ma được trích ly cùng với dung dịch NaCl 0,9% ở các tỷ lệ (w/v), thời gian và nhiệt độ ủ khác nhau. Dịch chiết thô được tinh sạch bằng phương pháp tủa phân đoạn với muối ammonium sulfate, tiếp theo là sắc ký ái lực trên gel Sepharose D-galactose để cải thiện độ tinh sạch. Kết quả cho thấy lectin đậu ma đạt hiệu quả trích ly tối ưu với hoạt tính đặc hiệu đạt là 1.579 HAA (Hemagglutination assay)/mg ở tỉ lệ với dung môi trích ly là 1:4 (w/v), tại 50oC, trong 10 phút. Dịch trích lectin đậu ma sau khi tủa phân đoạn ở nồng độ muối 40% - 50% cho hiệu suất thu hồi 35,4% với độ tinh sạch tăng 6,38 lần so với dịch trích thô; trong khi phân đoạn F1 từ sắc ký ái lực cho hiệu suất thu hồi 9,85% với độ tinh sạch tăng 16,2 lần. Kết quả điện di SDS-PAGE xuất hiện hai băng protein có khối lượng phân tử 66,0 kDa và 56,0 kDa.

Nódulos presentes en leguminosas asociadas a pasturas de Brachiaria sp en la Amazonia Colombiana

Se estudiaron 23 pasturas de Brachiaria sp dedicadas a la producción bovina doble propósito, para identificar especies de leguminosas presentes y obtener muestras de raíces para extracción y recuento de nódulos. Toda el área correspondió a un total de 149,14 ha, donde solo 84,26 ha contaban con la presencia de leguminosas Desmodium sp, Pueraria phaseoloides, Galactia striata, Arachis pintoi, Stylosanthes sp, Mimosa púdica y Centrosema sp, cuya cobertura fue del 2,89% de dicha área. La especie con mayor porcentaje de cobertura promedio fue A.pintoi con 5,36% seguida de Stylosanthes sp con 5%, G.striata con 3,89% y P.phaseoloides con 2,62%. Las restantes se encontraron en un rango inferior a 2% del área de pastura. Se halló en el estudio un mínimo de 7 nódulos en raíces de Centrosema sp y G.striata, así como un número máximo de 194 nódulos en Desmodium sp. Las cantidades superiores a 100 nódulos por planta solo correspondieron a Desmodium sp, el cual reveló una inoculación satisfactoria que se asoció con su potencial de fijación de nitrógeno mediada por simbiosis. La especie con menor número de nódulos fue M. púdica, hallada en el 4,3% de las pasturas evaluadas, siendo la única de las leguminosas muestreadas que no es consumida directamente por los bovinos y se desarrolla como especie no deseada, indicadora de estados de degradación de pasturas.

Effects of incorporation levels of Pueraria phaseoloides leaf flour on carcass characteristics and chemical composition of meat from local rabbit (Oryctolagus cuniculus) in South-East Gabon

The objective of this study was to contribute in improving knowledge on rabbit feeding in Gabon. This work was conducted with the aim of studying carcass characteristics and chemical composition of meat from rabbit induced by feeds containing P. phaseoloides. To that effect, 20 young female rabbits from local breed, weighing averagely 611 ± 33.20 g and aged about 6 weeks were randomly distributed in 4 groups of 5 animals each. The groups R0, R15, R20 and R25 were fed rations containing respectively 0%, 15%, 20% and 25% of P. phaseoloides. At the end of the trial, animals were slaughtered then eviscerated in order to evaluate carcass characteristics. Muscles were taken, ground and mixed for chemical analysis. No matter the ration, carcass characteristics showed no significant difference (p > 0.05). Highest protein contents in meat were recorded in animals from R15 (23.90% DM) and R20 (22.46% DM). The lowest fat content (5.41% DM) was that of animals from R25, followed by that from R20 (6.67% DM). Based on these results obtained, the ration containing 20% Pueraria phaseolides (R20) could be recommended.

Contribution of Pueraria phaseoloides L. in the Cycling of Macronutrients in oil Palm Plantations

The use of cover crops has become a common practice in oil palm plantations, ensuring greater sustainability to agroecosystems, increasing nutrient cycling and providing greater savings in the use of mineral fertilizers. In this sense, the objective of this study was to evaluate the accumulation of macronutrients (N, P, K, Ca, Mg and S) in tropical kudzu plants (Pueraria phaseoloides L.) in oil palm plantations. The experiment was carried out in the municipality of Tailândia, Pará State, using a completely randomized experimental design, four replications and seven treatments (ages of oil palm). The accumulation of macronutrients was obtained through the product of nutrient concentrations and the dry matter of the aerial part of the living, dead and total cover of tropical kudzu. The order of total accumulation of cycled nutrients by legumes was Ca, N, K, Mg, P and S. Tropical kudzu cultivation, as a cover plant, contributes to nutrient cycling in oil palm plantations, but this benefit decreased with the age of palm oil trees.

Pueraria phaseoloides (tropical kudzu).

P. phaseoloides is a vigorous fast-growing vine included in the Global Compendium of Weeds (Randall, 2012) and listed as one of the most aggressive weeds invading moist habitats in tropical and subtropical regions (USDA-ARS, 2012). It spreads by seeds and by runners (i.e., stolons) which are structures that enable plants to multiply rapidly and colonize entire forests very fast. This species has been extensively introduced in tropical and subtropical region of the world to be used as forage for livestock, to control soil erosion, and as a soil improvement species (Skerman et al., 1991; Cook et al., 2005). P. phaseoloides has the potential to degrade other plants by smothering them under a solid blanket of leaves, by girdling woody stems and tree trunks, and by breaking branches or uprooting entire trees and shrubs by the strength of its weight. Currently, this species is classified as a "noxious weed" in the United States (USDA-NRCS,2012) and as an invasive species in Costa Rica, Ecuador, Puerto Rico and Pacific Islands including Hawaii, Fiji, French Polynesia, Niue and New Caledonia (Soria et al., 2002; Acevedo-Rodríguez and Strong, 2012; Chacón and Saborio, 2012; PIER, 2012).

Performance and Carcass Analysis of Growing Snails Fed Calopogonium mucunoides (Calopo) And Pueraria phaseoloides (Kudzupuero)

An experiment was carried out to determine the effect of feeding growing snails with two different leguminous plants, Calopogonium muculoides, Pueraria phaseoloides, using pawpaw (Carica papaya) leaf as control. A total of 72 growing snails (Archachatina marginata) of mean weight 74.73/3.5g were used for the feeding trial. The snails were randomly alloted into 3 different groups and each group was replicated 4 times with 6 snails per replicate in a completely randomized design. Snails in T1 were fed pawpaw leaf (control), while snails in T2 were fed Calopogolium muculoides. Parameters measured were feed intake, weight gain, shell length and width and feed conversion ratio. The feeding trial lasted for 12 weeks. The results on growth performances reveal that the highest feed intake was recorded in snails fed pawpaw leaf (PL) which was similar to the those fed Pueraria phaseoloids (PP), while the lowest feed intake was recorded in snail fed with Calopognium muculoids (CM). The highest weight gain was also recorded in snails fed with pawpaw leaf and Peuraria phaseoloids (PP) than those fed Calopognium muculoides (CM). In conclusion, Pueraria phaseoloids could be used as substitute for pawpaw leaf.

Maize grain and straw yields over 14 consecutive years in burned and mulched Mucuna pruriens var. utilis and Pueraria phaseoloides relay cropping systems

The cover crops Mucuna pruriens var. utilis and Pueraria phaseoloides were introduced to African farmers to improve crop production on degraded soils, yet they appear not to be adopted at scale. In the humid forest zone of West and Central Africa, the dominant Acrisols and Nitisols are inherently poor even when not degraded through agriculture. In this zone, sole maize cropping and vegetable production systems are gaining importance, yet both suffer from nutrient deficiencies. Cover crops were often introduced along with a system change, requiring biomass retention, mainly for nutrient retention reasons. Farmers in the zone commonly use slash and burn systems due to added weed control and ease of operations on clean fields. This study evaluated mucuna and pueraria with and without burning the fallow biomass in an annual sole maize crop relay system against the burned and retained natural fallow. Over 14 consecutive years, biomass burning did not cause lower maize grain yields in any of the fallow types, on the contrary, the economically important marketable cob yields were higher when biomass was burned (mulched 2.10 cobs m−2 vs. 2.26 cobs m−2 when burned, p < 0.07). After cover crop fallow, maize grain yields were significantly higher than after natural fallow (1.92 Mg ha−1) over the 14 years, with maize yields in the pueraria treatment (2.63 Mg ha−1) out yielding those in the mucuna treatment (2.28 Mg ha−1). Maize produced 1.92 cobs m−2 in natural fallow, significantly less than in the mucuna (2.23 m−2, p < 0.013) and the pueraria (2.39 m−2, p < 0.001) fallow. Introducing mucuna or pueraria cover crops into slash and burn systems appears as a suitable measure to increase yields without changing the land preparation approach.

Determinación de la digestibilidad y energía digestible de la harina de kudzu (Pueraria phaseoloides) en el cuy (Cavia porcellus)

El experimento fue conducido para determinar los coeficientes de digestibilidad y la energía digestible de la harina de kudzu (Pueraria phaseoloides) en cuyes. Se utilizaron 10 cuyes machos de 12 semanas y 828 g de peso promedio, colocados en jaulas metabólicas. Cinco animales fueron alimentados con la dieta basal (subproducto de trigo) y los otros cinco con la dieta experimental (60% subproducto de trigo y 40% harina de kudzu). Para estas determinaciones se utilizó el método indirecto, teniendo como alimento basal al subproducto de trigo, cuyos coeficientes de digestibilidad se determinaron por el método directo. Se tuvo una fase pre-experimental de 14 días para la adaptación de los cuyes a las instalaciones y la nueva dieta. La fase experimental fue de seis días, en la cual se realizó la colección de heces. En ambas fases se midió diariamente el consumo de alimento. Los coeficientes de digestibilidad aparente obtenidos fueron: 42.90% de materia seca, 40.57% de materia orgánica, 81.07% de proteína cruda, 68.86% de extracto etéreo, 30.77% de fibra cruda, 30.55% de extracto libre de nitrógeno y 74.18% de ceniza. El valor de la energía digestible determinado por diferencia fue de 1.98 Mcal/kg de materia seca.

Leguminous species effect in agroforestry systems in the Portuguesa state. Venezuela

Six leguminous species Frijol bayo (Vigna unguiculata), Frijol white Var. Orituco, Quinchoncho dwarf (Cajanus cajan (L.) Millsp., cv. Aroita), Kudzú tropical (Pueraria phaseoloides), Crotalaria (Crotalaria juncea), Sesbania (Sesbania grandiflora) were established in an agroforest systems to assess his effect on the chemical property of a Alfisol soil at municipality Ospino Portuguese state, at the farm La Yaguara planted with E. urograndis. In a design in random blocks with 7 processings and 4 repetitions, employing STATIXTIS 9.0 to carry out the ANDEVA and for variables where differences are presented the test was employed of Tukey (5%); obtaining as results that in the soil of the farm The Yaguara alone significant differences were found (P<0,05) for the variables relation C/N, CO, Zn, highly significant and differences (P<0,01)for N; being the Sesbania and quinchoncho the best treatment in relation C/N; for CO the frijol blanco and the treatment control; for Zn the quinchoncho and treatment control, frijol blanco and kudzú tropical; in the N quinchoncho and Sesbania. To confirm the generated profit to the agroforestry system analysis were completed foliate to the eucalyptus, finding highly significant differences for P with Frijol blanco and Kudzú and significant for Zn where is quinchoncho and treatment control.