Strategies for the selection of uncontaminatedEucalyptusexplants for shoot multiplication in a temporary immersion system (RITA®) in a commercial laboratory

Date palm micropropagation is commonly performed on gelled media. However, it’s typically a labour-intensive system and consequently plantlets production cost is very high. Therefore, it is necessary to develop cost effective alternatives without compromising the quality of produced plant material. New technologies based on liquid media in bioreactors have been developed to reduce the handling time, while increasing the multiplication rates and plant quality. The present research focuses on the comparison between Temporary Immersion System (TIS) and gelled media (GM) culture systems of two Moroccan date palm varieties ‘Mejhool’ and ‘Boufeggous’. Obtained results indicated that shoot and root lengths as well as shoot fresh and dry weights were significantly (P < 0.05) higher in TIS compared to GM. Moreover, the vigour of obtained shoots was better in TIS compared to GM. Therefore, TIS-derived plantlets have shown an acclimatization rate of 95% while this rate for GM-derived plantlets was 82%. Hence, bioreactors, as a growing system based on TIS, can be a valid alternative to conventional systems for in vitro culture, resulting in a reduction of cost, shelving area requirements, labour and time for the mass propagation of date palm cultivars.

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Shoot multiplication and growth rates of Aquilaria malaccensis Lamk. shoot cultures in temporary immersion system (TIS)-RITA® and bubble column bioreactors

Pakistan Journal of Botany ◽

10.30848/pjb2019-4(36) ◽

2019 ◽

Vol 51 (4) ◽

Author(s):

Rizkita Rachmi Esyanti ◽

Mochammad Fadholi ◽

Rifki Muhammad Rizki ◽

Ahmad Faizal

Keyword(s):

Growth Rates ◽

Bubble Column ◽

Shoot Multiplication ◽

Shoot Cultures ◽

Temporary Immersion System ◽

Temporary Immersion ◽

Aquilaria Malaccensis

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In Vitro Culture of Rosmarinus officinalis L. in a Temporary Immersion System: Influence of Two Phytohormones on Plant Growth and Carnosol Production

Pharmaceuticals ◽

10.3390/ph14080747 ◽

2021 ◽

Vol 14 (8) ◽

pp. 747

Author(s):

Eder Villegas Sánchez ◽

Mariana Macías-Alonso ◽

Soraya Osegueda Robles ◽

Lisset Herrera-Isidrón ◽

Hector Nuñez-Palenius ◽

...

Keyword(s):

High Performance ◽

Emerging Infectious Diseases ◽

Rosmarinus Officinalis ◽

Wild Plant ◽

Array Detector ◽

Phase System ◽

Naphthaleneacetic Acid ◽

Temporary Immersion System ◽

Temporary Immersion

Emerging infectious diseases have become a major global problem with public health and economic consequences. It is an urgent need to develop new anti-infective therapies. The natural diterpene carnosol exhibit a wide variety of interesting antibacterial and antiviral properties, and it is considered a theoretical inhibitor of COVID-19 Mpro. However, this compound is present in the family Lamiaceae in low quantities. To obtain carnosol in concentrations high enough to develop pharmacological studies, we evaluated the efficiency of a micropropagation protocol of Rosmarinus officinalis using a solid medium and a temporary immersion system (TIS), as well as the effect of 6-benzylaminopurine (6-BAP) and α-naphthaleneacetic acid (NAA) on the growth of shoots. Moreover, we developed and validated an analytical method to quantify carnosol using the H-point standard additions method in the high-performance liquid chromatography diode array detector (HPLC-DAD). After 30 days of culture, TIS produced the maximum number of shoots per explant (24.33 ± 1.15) on a liquid medium supplemented with 6-BAP at 5.0 mg L−1. Next, we also evaluated the effect of immersion time and frequency for TIS. After 72 days of culture, the best results were obtained with an immersion cycle of 1 min every 12 h, yielding 170.33 ± 29.40 shoots. The quantification of carnosol on the samples was performed at a flow rate of 1.2 mL min−1 using binary isocratic mobile phase system 60:40 (v/v) 10 mM formic acid (pH 3.0) (A) and acetonitrile (B) on a reverse-phase column. The content of carnosol in the in vitro cultures was around 8-fold higher than in the wild plant. The present study represents an efficient alternative method to obtain carnosol for its pre-clinical and clinical development.

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