Liposome assisted drug delivery-A review

Liposomes, sphere-formed vesicles consisting of one or greater phospholipid bilayers, had been first described within the mid-60s. Among numerous gifted new drug delivery systems, liposomes signify an advanced generation to supply active molecules to the site of action, and right now, numerous formulations are in clinical use. The application of liposomes to help drug shipping has already had a chief impact on many biomedical regions. They have been proven to be beneficial for stabilizing pharmaceuticals, overcoming boundaries to cellular and tissue uptake, and improving biodistribution of compounds to goal sites In vivo. This permits powerful delivery of encapsulated compounds to goal sites even as minimizing systemic toxicity. Liposomes present as an attractive transport gadget due to their wide physicochemical and biophysical properties which allow smooth manipulation to cope with exclusive shipping concerns. In this review, we will talk the advances in liposome assisted drug shipping, biological challenges, and present day medical and experimental use of liposomes for biomedical applications. The translational limitations of liposomal technology may also be provided.

On the Prospects of Using the Handwriting Analysis of a Structured Text in Law Enforcement Work

The authors examine some problematic aspects of the practical application of the developed forensic methods and methodologies in law enforcement work. They discuss the possibilities of studying handwriting not only for identification, but also diagnostic purposes, for example, to determine the significance of information in certain parts of the handwritten text for the writer. In order to prove that such possibilities exist, the authors present a brief description and examples of the experimental use of the methodology that makes it possible to determine, relatively quickly, the attitude of the person to relevant circumstances and facts by analyzing experimental samples of handwriting obtained through copying, by hand, a specially prepared structured text.

USING OF INFORMATION AND METHODOLOGICAL PROVISIONS BY STUDENTS OF VOCATIONAL EDUCATION INSTITUTIONS

The purpose of the article is to substantiate the system of information and methodological support for students of vocational education institutions. The authors analyse the ways of training junior bachelors of speciality 121 “Software Engineering”, taking into account the development of information technologies and changes in science, technology, technologies in the legal framework of economics and education.The author found out that the educational process is inadequately provided with educational and methodical materials aimed at the formation of design skills in the field of software development. The content of information activities of junior bachelors on the use of information and methodological support is developed. It consists of the following: development of algorithms that implement the content, and technological components of the content of information and methodological support; development of the code and adjustment of the applied program of information and methodical maintenance; the experimental use of information and methodological support in the educational process; adjusting the information and methodological support based on the results of experimental use; the introduction of information and methodological support in the educational process of the institution of professional higher education; the development of methodological documentation for users of information and methodological support; the development of methodological documentation for a training seminar for potential users of the developed information and methodological support; the design of the results of interdisciplinary design. Keywords: information and methodological support; institutions of professional higher education; professional education; educational process; information and educational environment; software engineering; digital competence; information system.

Delay in assessing vitamin D supplements and live vaccines for tuberculosis, polio, and measles to protect people from Covid-19

Introduction: In March 2020, less than three months after China reported a cluster of pneumonia cases in Wuhan, the United States (US) government budgeted money to support development of Covid-19 vaccines. By mid-December 2020, two had been developed, tested, and received the US government’s experimental use authorization. Given evidence that vitamin D supplements and live vaccines for tuberculosis, polio, and measles reduce risks for acute respiratory infection, many experts hypothesized they might reduce risks for Covid-19 infection. Expedited randomized controlled trials, as done for Covid-19 vaccines, could have assessed their protection against C19 no later than end-July 2020. Methods: On 21 April 2021, I searched trial registries maintained by the US National Institutes of Medicine and the World Health Organization for trials with ≥400 participants to assess vitamin D or live vaccines to prevent Covid-19 infections (all or symptomatic). On 10-13 November 2021, I searched PubMed and medRxiv for results reported from these trials.Results: In April 2021, I found 32 trials (9 for vitamin D and 23 for live vaccines) proposing to assess the impact of these interventions on rates of new Covid-19 infections (all or symptomatic). Only 10 trials proposed to begin by June 2020, and only one to end in 2020. My search on 10-13 November 2021, almost 11 months after the US approved the first two Covid-19 vaccines, found results reported from only one of the 32 trials (live measles vaccine significantly reduced new symptomatic infections). Conclusions: If health experts had demonstrated similar urgency in assessing vitamin D supplements and live vaccines for tuberculosis, polio, and measles as in developing Covid-19 vaccines, trials could have reported by end-July 2020. Depending on what trials reported, these interventions could have prevented a large percentage of more than 600,000 Covid-19 deaths reported in the US from August 2020 through November 2021. Delay in assessing vitamin D has racial implications as well, since vitamin D deficiency and Covid-19 deaths in the US have been far more common among Blacks and Hispanics compared to Whites. Going forward, depending on what trials report, these interventions could help people live with Covid-19 as an endemic virus.

Boy with Machine

In this paper, I provide an analysis of the anime series Neon Genesis Evangelion and the feature film The End of Evangelion through the theory of French philosophers Gilles Deleuze and Félix Guattari as outlined in their seminal work Capitalism and Schizophrenia. I tackle the authors’ concepts of Oedipus and absolute deterritorialization in order to provide a philosophical consideration of the series’ central plot points and developments. My aim is to employ Charles J. Stivale’s concept of academic “animation” to critique Evangelion’s emphasis on the nuclear family structure and its influence on subject-formation, as well as to demonstrate that a Deleuzoguattarian framework is uniquely suited for this task. I conclude that Evangelion, through its experimental use of animation as a medium, produces a compelling depiction of absolute deterritorialization in the form of the Human Instrumentality Project. However, the series ultimately remains loyal to its prioritisation (rooted in psychoanalysis and the Oedipus complex) of the family unit, with the protagonist Ikari Shinji rejecting Instrumentality and preferring, instead, to live as a unified subject defined by familial relations.

A Review of High-Intensity Focused Ultrasound in Urology

This review provides an introduction to high-intensity focused ultrasound (HIFU) and reviews its historical and current use in urological surgery. Current and historical literature (1927–2020), including that describing trials and review articles in the medical and ultrasonic literature, has been reviewed, using Pub Med and Cochrane search engines. HIFU is currently one of a number of treatments for prostate cancer, both as a primary treatment that can be repeated, and as a salvage treatment post-radiotherapy. HIFU is not yet sufficiently mature to be a standard treatment for renal cancer or other urological diseases, although there has been some success in early clinical trials. As the technology improves, this situation is likely to change. HIFU has been understood as a concept for a century, and has been applied in experimental use for half that time. It is now an accepted treatment with low morbidity in many diseases outside the scope of this review. In urological surgery, prostate HIFU is accepted as a localised treatment in selected cases, with potentially fewer side effects than other localised therapies. Currently the treatment for renal cancer is hindered by the perinephric fat and the position of the kidneys behind the ribs; however, as the technology improves with image fusion, faster treatments, and the ability with phased array transducers and motion compensation to overcome the problems caused by the ribs and breathing, successful treatment of kidney tumours will become more of a reality. In due course, there will be a new generation of machines for treating prostate cancer. These devices will further minimise the side effects of radical treatment of prostate cancer.

Stability of extemporaneously prepared preservative-free methylphenidate 5 mg/mL intravenous solution

Disclaimer In an effort to expedite the publication of articles , AJHP is posting manuscripts online as soon as possible after acceptance. Accepted manuscripts have been peer-reviewed and copyedited, but are posted online before technical formatting and author proofing. These manuscripts are not the final version of record and will be replaced with the final article (formatted per AJHP style and proofed by the authors) at a later time. Purpose To advance the implementation of consciousness-promoting therapies in patients with acute disorders of consciousness, the availability of potential therapeutic agents in formulations suitable for administration in hospitalized patients in the presence of complex comorbid conditions is paramount. The purpose of this study is to evaluate the long-term stability of extemporaneously prepared preservative-free methylphenidate hydrochloride (HCl) 5 mg/mL intravenous solution for experimental use. Methods A methylphenidate 5 mg/mL solution was prepared under proper aseptic techniques with Methylphenidate Hydrochloride, USP, powder mixed in sterile water for solution. Methylphenidate HCl 5 mg/mL solution was sterilized by filtration technique under USP <797>–compliant conditions. Samples were stored refrigerated (2-8°C) and analyzed at approximately days 1, 30, 60, 90, 180, and 365. At each time point, chemical and physical stability were evaluated by visual inspection, pH measurement, membrane filtration procedure, turbidometric or photometric technique, and high-performance liquid chromatography analysis. Results Over the 1-year study period, the samples retained 96.76% to 102.04% of the initial methylphenidate concentration. There was no significant change in the visual appearance, pH level, or particulate matter during the study period. The sterility of samples was maintained and endotoxin levels were undetectable throughout the 1-year stability period. Conclusion Extemporaneously prepared preservative-free methylphenidate 5 mg/mL intravenous solution was physically and chemically stable at 32, 61, 95, 186, and 365 days when stored in amber glass vials at refrigerated temperatures (2-8°C).

Functional analysis of Middle Palaeolithic flint tools through experimental use wear analysis: the case of Prado Vargas (Cornejo) and Fuente Mudarra (Atapuerca), northern Spain

There are functional differences related to the peculiarities of each settlement. The material used to manufacture tools is one of the key factors in the analysis of use-wear traces in traceological studies. An experiment was conducted to test the development of these functional traces in two types of flints found at two Middle Palaeolithic settlements: Neogene flint, from the Fuente Mudarra open-air site Sierra de Atapuerca, and Cretaceous flint, from the Prado Vargas cave site, Ojo Guareña. After reviewing the characteristic of each type of flint, they were compared to previous archaeological studies in order to check the reliability of the analysis of these settlements.

Activation of GPER limits adverse changes to Ca2+ signalling and arrhythmogenic activity in cardiomyocytes of ovariectomised guinea pigs

Background Post-menopausal women have an enhanced risk of developing cardiovascular disease and disturbances of cardiac rhythm, generally attributed to declining oestrogen levels during menopause. In an animal model that mimics menopause, the long-term withdrawal of oestrogen dysregulated Ca2+ signalling and increased the formation of a pro-arrhythmic substrate. Selective targeting of the membrane bound G-protein coupled oestrogen receptor 1 (GPER) eliminated such arrhythmogenic activity associated with the loss of oestrogen. Purpose We aim to assess the “cardioprotective” role of GPER in response to oestrogen withdrawal. Methods Ovariectomy (OVx) or sham surgeries were conducted on female guinea pigs. Left ventricular cardiomyocytes were isolated 150-days post-operatively for experimental use. GPER expression was quantified by western blot. Myocytes were incubated in solutions containing GPER agonist G-1 for >2h before recording some electrophysiological and Ca2+ regulatory parameters. Results GPER expression was 32% higher in OVx. OVx cardiomyocytes had prolonged action potential duration (APD) compared with sham and in the presence of G-1, APD90 shortened by 12% and 22% in sham and OVx, respectively. G-1 reduced early after depolarisation (EAD) formation by >99% in OVx. OVx cells had larger sarcoplasmic reticulum (SR) Ca2+ content (by 13%), compared with sham. While G-1 had little effect on SR content, it reduced Ca2+ transient amplitude (by 40%), SR fractional release (by 11%) and sarcomere shortening (by 29%) in OVx cells. The frequency of occurrence of spontaneous Ca2+ waves evoked by periods of rapid stimulation reduced by 40% and wave-free survival time prolonged in OVx cells incubated with G-1. Conclusions In the hearts of an animal species whose electrophysiology and intracellular Ca2+ regulation is akin to humans, we show that following oestrogen deficiency, GPER expression increased and its activation induces negative inotropic responses in cardiomyocytes. It limits the adverse changes to Ca2+ signalling and induces anti-arrhythmogenic behaviours in OVx. FUNDunding Acknowledgement Type of funding sources: Private grant(s) and/or Sponsorship. Main funding source(s): British Heart Foundation