scholarly journals Evaluation of Two Housekeeping Gene in Diagnosis of Helicobacter Pylori and Compare It to another Routine Test

2021 ◽  
Author(s):  
Saja A. Al-Jumaili ◽  
Amina N. Althwani ◽  
Nawal M. Al-Khalidi
Keyword(s):  
Helicobacter Pylori ◽  
Housekeeping Gene ◽  
Routine Test

scholarly journals Detection of Helicobacter pylori Microevolution and Multiple Infection from Gastric Biopsies by Housekeeping Gene Amplicon Sequencing

Pathogens ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 97
Author(s):  
Montserrat Palau ◽  
Núria Piqué ◽  
André M. Comeau ◽  
Gavin M. Douglas ◽  
M. José Ramírez-Lázaro ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Pcr Amplification ◽  
Housekeeping Genes ◽  
Housekeeping Gene ◽  
Amplicon Sequencing ◽  
Multiple Infection ◽  
Multiple Infections ◽  
Single Strain ◽  
Gastric Biopsies ◽  
H Pylori

Despite the great efforts devoted to research on Helicobacter pylori, the prevalence of single-strain infection or H. pylori mixed infection and its implications in the mode of transmission of this bacterium are still controversial. In this study, we explored the usefulness of housekeeping gene amplicon sequencing in the detection of H. pylori microevolution and multiple infections. DNA was extracted from five gastric biopsies from four patients infected with distinct histopathological diagnoses. PCR amplification of six H. pylori-specific housekeeping genes was then assessed on each sample. Optimal results were obtained for the cgt and luxS genes, which were selected for amplicon sequencing. A total of 11,833 cgt and 403 luxS amplicon sequences were obtained, 2042 and 112 of which were unique sequences, respectively. All cgt and luxS sequences were clustered at 97% to 9 and 13 operational taxonomic units (OTUs), respectively. For each sample from a different patient, a single OTU comprised the majority of sequences in both genes, but more than one OTU was detected in all samples. These results suggest that multiple infections with a predominant strain together with other minority strains are the main way by which H. pylori colonizes the human stomach.

The rod-to-coccoid body transformation in cultures of Helicobacter pylori

1990 ◽  
Vol 48 (3) ◽  
pp. 626-627
Author(s):  
A. R. Crooker ◽  
W. G. Kraft ◽  
T. L. Beard ◽  
M. C. Myers
Keyword(s):  
Helicobacter Pylori ◽  
Growth Cycle ◽  
Negative Bacterium ◽  
Body Formation ◽  
Coccoid Form ◽  
Spiral Form ◽  
Prolonged Culture ◽  
H Pylori ◽  
Upper Gastrointestinal

Helicobacter pylori is a microaerophilic, gram-negative bacterium found in the upper gastrointestinal tract of humans. There is strong evidence that H. pylori is important in the etiology of gastritis; the bacterium may also be a major predisposing cause of peptic ulceration. On the gastric mucosa, the organism exists as a spiral form with one to seven sheathed flagella at one (usually) or both poles. Short spirals were seen in the first successful culture of the organism in 1983. In 1984, Marshall and Warren reported a coccoid form in older cultures. Since that time, other workers have observed rod and coccal forms in vitro; coccoid forms predominate in cultures 3-7 days old. We sought to examine the growth cycle of H. pylori in prolonged culture and the mode of coccoid body formation.

DYSPEPSIA SYNDROME AND HELICOBACTER PYLORI INFECTION IN END STAGE RENAL FAILURE

2000 ◽  
Vol 15 (12) ◽  
pp. H2-H2
Author(s):  
IS Mertasudira ◽  
JR Saketi ◽  
A. Djumhana ◽  
J. Widjojo ◽  
SA Abdurachman
Keyword(s):  
Renal Failure ◽  
Helicobacter Pylori ◽  
Helicobacter Pylori Infection ◽  
End Stage Renal Failure ◽  
End Stage

Mouse model of gastric infection with cytotoxin-expressing strain of Helicobacter pylori in studying of pathogenesis of chronic gastric ulcer

1998 ◽  
Vol 13 (11-s4) ◽  
pp. S178-S184 ◽  
Author(s):  
PETER KONTUREK ◽  
TOMASZ BRZOZOWSKI ◽  
STANISLAW KONTUREK ◽  
ELZBIETA KARCZEWSKA ◽  
ROBERT PAJDO ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Ulcer ◽  
Mouse Model ◽  
Chronic Gastric Ulcer

HEMATEMESIS AND MELENA IN BABY WITH HELICOBACTER PYLORI INFECTION

2000 ◽  
Vol 15 (12) ◽  
pp. H10-H11
Author(s):  
Budi Purnomo ◽  
Eva J. Soeleman ◽  
Hadjat S. Digdowirogo ◽  
Hartati N. Soehardjo
Keyword(s):  
Helicobacter Pylori ◽  
Helicobacter Pylori Infection
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